RAPID ALKALINIZATION FACTOR 22 is a key modulator of the root hair growth responses to fungal ethylene emissions in Arabidopsis

Autores
Morcillo, Rafael Jorge León; Leal López, Jesús; Férez Gómez, Alberto; López Serrano, Lidia; Baroja Fernández, Edurne; Gámez Arcas, Samuel; Tortosa, Germán; Lopez, Leonel Emanuel; Estevez, Jose Manuel; Doblas, Verónica G.; Frías España, Laura; Garcia Pedrajas, Maria Dolores; Sarmiento Villamil, Jorge Luis; Pozueta Romero, Javier
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In Arabidopsis (Arabidopsis thaliana (L.) Heynh), exposure to volatile compounds (VCs) emitted by Penicillium aurantiogriseum promotes root hair (RH) proliferation and hyper-elongation through mechanisms involving ethylene, auxin, and photosynthesis signaling. In addition, this treatment enhances the levels of the small signaling peptide RAPID ALKALINIZATION FACTOR 22 (RALF22). Here, we used genetics to address the role of RALF22 in fungal VC-promoted RH growth and to identify the bioactive fungal VC. We found that RHs of ralf22 and feronia (fer-4) plants impaired in the expression of RALF22 and its receptor FERONIA, respectively, responded weakly to fungal VCs. Unlike in wild-type roots, fungal VC exposure did not enhance RALF22 transcript levels in roots of fer-4 and ethylene- and auxin-insensitive mutants. In ralf22 and fer-4 roots, this treatment did not enhance the levels of ERS2 transcripts encoding one member of the ethylene receptor family and those of some RH-related genes. RHs of ers2-1 and the rsl2rsl4 double mutants impaired in the expression of ERS2 and the ethylene- and auxin-responsive ROOT HAIR DEFECTIVE 6-LIKE 2 and 4 transcription factors, respectively, weakly responded to fungal VCs. Moreover, roots of plants defective in photosynthetic responsiveness to VCs exhibited weak RALF22 expression and RH growth responses to fungal VCs. VCs of ΔefeA strains of P. aurantiogriseum cultures impaired in ethylene synthesis weakly promoted RH proliferation and elongation in exposed plants. We conclude that RALF22 simultaneously functions as a transcriptionally regulated signaling molecule that participates in the ethylene, auxin, and photosynthesis signaling-mediated RH growth response to fungal ethylene emissions and regulation of ethylene perception in RHs.
Fil: Morcillo, Rafael Jorge León. Consejo Superior de Investigaciones Científicas; España
Fil: Leal López, Jesús. Consejo Superior de Investigaciones Científicas; España
Fil: Férez Gómez, Alberto. Consejo Superior de Investigaciones Científicas; España
Fil: López Serrano, Lidia. Consejo Superior de Investigaciones Científicas; España
Fil: Baroja Fernández, Edurne. Consejo Superior de Investigaciones Científicas; España
Fil: Gámez Arcas, Samuel. Consejo Superior de Investigaciones Científicas; España
Fil: Tortosa, Germán. Consejo Superior de Investigaciones Científicas; España
Fil: Lopez, Leonel Emanuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Estevez, Jose Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Doblas, Verónica G.. Consejo Superior de Investigaciones Científicas; España
Fil: Frías España, Laura. Consejo Superior de Investigaciones Científicas; España
Fil: Garcia Pedrajas, Maria Dolores. Consejo Superior de Investigaciones Científicas; España
Fil: Sarmiento Villamil, Jorge Luis. Consejo Superior de Investigaciones Científicas; España
Fil: Pozueta Romero, Javier. Consejo Superior de Investigaciones Científicas; España
Materia
Arabidopsis
RALF
etileno
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/291132
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/291132
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling RAPID ALKALINIZATION FACTOR 22 is a key modulator of the root hair growth responses to fungal ethylene emissions in ArabidopsisMorcillo, Rafael Jorge LeónLeal López, JesúsFérez Gómez, AlbertoLópez Serrano, LidiaBaroja Fernández, EdurneGámez Arcas, SamuelTortosa, GermánLopez, Leonel EmanuelEstevez, Jose ManuelDoblas, Verónica G.Frías España, LauraGarcia Pedrajas, Maria DoloresSarmiento Villamil, Jorge LuisPozueta Romero, JavierArabidopsisRALFetilenohttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In Arabidopsis (Arabidopsis thaliana (L.) Heynh), exposure to volatile compounds (VCs) emitted by Penicillium aurantiogriseum promotes root hair (RH) proliferation and hyper-elongation through mechanisms involving ethylene, auxin, and photosynthesis signaling. In addition, this treatment enhances the levels of the small signaling peptide RAPID ALKALINIZATION FACTOR 22 (RALF22). Here, we used genetics to address the role of RALF22 in fungal VC-promoted RH growth and to identify the bioactive fungal VC. We found that RHs of ralf22 and feronia (fer-4) plants impaired in the expression of RALF22 and its receptor FERONIA, respectively, responded weakly to fungal VCs. Unlike in wild-type roots, fungal VC exposure did not enhance RALF22 transcript levels in roots of fer-4 and ethylene- and auxin-insensitive mutants. In ralf22 and fer-4 roots, this treatment did not enhance the levels of ERS2 transcripts encoding one member of the ethylene receptor family and those of some RH-related genes. RHs of ers2-1 and the rsl2rsl4 double mutants impaired in the expression of ERS2 and the ethylene- and auxin-responsive ROOT HAIR DEFECTIVE 6-LIKE 2 and 4 transcription factors, respectively, weakly responded to fungal VCs. Moreover, roots of plants defective in photosynthetic responsiveness to VCs exhibited weak RALF22 expression and RH growth responses to fungal VCs. VCs of ΔefeA strains of P. aurantiogriseum cultures impaired in ethylene synthesis weakly promoted RH proliferation and elongation in exposed plants. We conclude that RALF22 simultaneously functions as a transcriptionally regulated signaling molecule that participates in the ethylene, auxin, and photosynthesis signaling-mediated RH growth response to fungal ethylene emissions and regulation of ethylene perception in RHs.Fil: Morcillo, Rafael Jorge León. Consejo Superior de Investigaciones Científicas; EspañaFil: Leal López, Jesús. Consejo Superior de Investigaciones Científicas; EspañaFil: Férez Gómez, Alberto. Consejo Superior de Investigaciones Científicas; EspañaFil: López Serrano, Lidia. Consejo Superior de Investigaciones Científicas; EspañaFil: Baroja Fernández, Edurne. Consejo Superior de Investigaciones Científicas; EspañaFil: Gámez Arcas, Samuel. Consejo Superior de Investigaciones Científicas; EspañaFil: Tortosa, Germán. Consejo Superior de Investigaciones Científicas; EspañaFil: Lopez, Leonel Emanuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Estevez, Jose Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Doblas, Verónica G.. Consejo Superior de Investigaciones Científicas; EspañaFil: Frías España, Laura. Consejo Superior de Investigaciones Científicas; EspañaFil: Garcia Pedrajas, Maria Dolores. Consejo Superior de Investigaciones Científicas; EspañaFil: Sarmiento Villamil, Jorge Luis. Consejo Superior de Investigaciones Científicas; EspañaFil: Pozueta Romero, Javier. Consejo Superior de Investigaciones Científicas; EspañaAmerican Society of Plant Biologist2024-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/291132Morcillo, Rafael Jorge León; Leal López, Jesús; Férez Gómez, Alberto; López Serrano, Lidia; Baroja Fernández, Edurne; et al.; RAPID ALKALINIZATION FACTOR 22 is a key modulator of the root hair growth responses to fungal ethylene emissions in Arabidopsis; American Society of Plant Biologist; Plant Physiology; 196; 4; 12-2024; 2890-29040032-0889CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/plphys/article/196/4/2890/7758768info:eu-repo/semantics/altIdentifier/doi/10.1093/plphys/kiae484info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-08-25T15:32:12Zoai:ri.conicet.gov.ar:11336/291132instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-08-25 15:32:13.289CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv RAPID ALKALINIZATION FACTOR 22 is a key modulator of the root hair growth responses to fungal ethylene emissions in Arabidopsis
title RAPID ALKALINIZATION FACTOR 22 is a key modulator of the root hair growth responses to fungal ethylene emissions in Arabidopsis
spellingShingle RAPID ALKALINIZATION FACTOR 22 is a key modulator of the root hair growth responses to fungal ethylene emissions in Arabidopsis
Morcillo, Rafael Jorge León
Arabidopsis
RALF
etileno
title_short RAPID ALKALINIZATION FACTOR 22 is a key modulator of the root hair growth responses to fungal ethylene emissions in Arabidopsis
title_full RAPID ALKALINIZATION FACTOR 22 is a key modulator of the root hair growth responses to fungal ethylene emissions in Arabidopsis
title_fullStr RAPID ALKALINIZATION FACTOR 22 is a key modulator of the root hair growth responses to fungal ethylene emissions in Arabidopsis
title_full_unstemmed RAPID ALKALINIZATION FACTOR 22 is a key modulator of the root hair growth responses to fungal ethylene emissions in Arabidopsis
title_sort RAPID ALKALINIZATION FACTOR 22 is a key modulator of the root hair growth responses to fungal ethylene emissions in Arabidopsis
dc.creator.none.fl_str_mv Morcillo, Rafael Jorge León
Leal López, Jesús
Férez Gómez, Alberto
López Serrano, Lidia
Baroja Fernández, Edurne
Gámez Arcas, Samuel
Tortosa, Germán
Lopez, Leonel Emanuel
Estevez, Jose Manuel
Doblas, Verónica G.
Frías España, Laura
Garcia Pedrajas, Maria Dolores
Sarmiento Villamil, Jorge Luis
Pozueta Romero, Javier
author Morcillo, Rafael Jorge León
author_facet Morcillo, Rafael Jorge León
Leal López, Jesús
Férez Gómez, Alberto
López Serrano, Lidia
Baroja Fernández, Edurne
Gámez Arcas, Samuel
Tortosa, Germán
Lopez, Leonel Emanuel
Estevez, Jose Manuel
Doblas, Verónica G.
Frías España, Laura
Garcia Pedrajas, Maria Dolores
Sarmiento Villamil, Jorge Luis
Pozueta Romero, Javier
author_role author
author2 Leal López, Jesús
Férez Gómez, Alberto
López Serrano, Lidia
Baroja Fernández, Edurne
Gámez Arcas, Samuel
Tortosa, Germán
Lopez, Leonel Emanuel
Estevez, Jose Manuel
Doblas, Verónica G.
Frías España, Laura
Garcia Pedrajas, Maria Dolores
Sarmiento Villamil, Jorge Luis
Pozueta Romero, Javier
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Arabidopsis
RALF
etileno
topic Arabidopsis
RALF
etileno
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In Arabidopsis (Arabidopsis thaliana (L.) Heynh), exposure to volatile compounds (VCs) emitted by Penicillium aurantiogriseum promotes root hair (RH) proliferation and hyper-elongation through mechanisms involving ethylene, auxin, and photosynthesis signaling. In addition, this treatment enhances the levels of the small signaling peptide RAPID ALKALINIZATION FACTOR 22 (RALF22). Here, we used genetics to address the role of RALF22 in fungal VC-promoted RH growth and to identify the bioactive fungal VC. We found that RHs of ralf22 and feronia (fer-4) plants impaired in the expression of RALF22 and its receptor FERONIA, respectively, responded weakly to fungal VCs. Unlike in wild-type roots, fungal VC exposure did not enhance RALF22 transcript levels in roots of fer-4 and ethylene- and auxin-insensitive mutants. In ralf22 and fer-4 roots, this treatment did not enhance the levels of ERS2 transcripts encoding one member of the ethylene receptor family and those of some RH-related genes. RHs of ers2-1 and the rsl2rsl4 double mutants impaired in the expression of ERS2 and the ethylene- and auxin-responsive ROOT HAIR DEFECTIVE 6-LIKE 2 and 4 transcription factors, respectively, weakly responded to fungal VCs. Moreover, roots of plants defective in photosynthetic responsiveness to VCs exhibited weak RALF22 expression and RH growth responses to fungal VCs. VCs of ΔefeA strains of P. aurantiogriseum cultures impaired in ethylene synthesis weakly promoted RH proliferation and elongation in exposed plants. We conclude that RALF22 simultaneously functions as a transcriptionally regulated signaling molecule that participates in the ethylene, auxin, and photosynthesis signaling-mediated RH growth response to fungal ethylene emissions and regulation of ethylene perception in RHs.
Fil: Morcillo, Rafael Jorge León. Consejo Superior de Investigaciones Científicas; España
Fil: Leal López, Jesús. Consejo Superior de Investigaciones Científicas; España
Fil: Férez Gómez, Alberto. Consejo Superior de Investigaciones Científicas; España
Fil: López Serrano, Lidia. Consejo Superior de Investigaciones Científicas; España
Fil: Baroja Fernández, Edurne. Consejo Superior de Investigaciones Científicas; España
Fil: Gámez Arcas, Samuel. Consejo Superior de Investigaciones Científicas; España
Fil: Tortosa, Germán. Consejo Superior de Investigaciones Científicas; España
Fil: Lopez, Leonel Emanuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Estevez, Jose Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Doblas, Verónica G.. Consejo Superior de Investigaciones Científicas; España
Fil: Frías España, Laura. Consejo Superior de Investigaciones Científicas; España
Fil: Garcia Pedrajas, Maria Dolores. Consejo Superior de Investigaciones Científicas; España
Fil: Sarmiento Villamil, Jorge Luis. Consejo Superior de Investigaciones Científicas; España
Fil: Pozueta Romero, Javier. Consejo Superior de Investigaciones Científicas; España
description In Arabidopsis (Arabidopsis thaliana (L.) Heynh), exposure to volatile compounds (VCs) emitted by Penicillium aurantiogriseum promotes root hair (RH) proliferation and hyper-elongation through mechanisms involving ethylene, auxin, and photosynthesis signaling. In addition, this treatment enhances the levels of the small signaling peptide RAPID ALKALINIZATION FACTOR 22 (RALF22). Here, we used genetics to address the role of RALF22 in fungal VC-promoted RH growth and to identify the bioactive fungal VC. We found that RHs of ralf22 and feronia (fer-4) plants impaired in the expression of RALF22 and its receptor FERONIA, respectively, responded weakly to fungal VCs. Unlike in wild-type roots, fungal VC exposure did not enhance RALF22 transcript levels in roots of fer-4 and ethylene- and auxin-insensitive mutants. In ralf22 and fer-4 roots, this treatment did not enhance the levels of ERS2 transcripts encoding one member of the ethylene receptor family and those of some RH-related genes. RHs of ers2-1 and the rsl2rsl4 double mutants impaired in the expression of ERS2 and the ethylene- and auxin-responsive ROOT HAIR DEFECTIVE 6-LIKE 2 and 4 transcription factors, respectively, weakly responded to fungal VCs. Moreover, roots of plants defective in photosynthetic responsiveness to VCs exhibited weak RALF22 expression and RH growth responses to fungal VCs. VCs of ΔefeA strains of P. aurantiogriseum cultures impaired in ethylene synthesis weakly promoted RH proliferation and elongation in exposed plants. We conclude that RALF22 simultaneously functions as a transcriptionally regulated signaling molecule that participates in the ethylene, auxin, and photosynthesis signaling-mediated RH growth response to fungal ethylene emissions and regulation of ethylene perception in RHs.
publishDate 2024
dc.date.none.fl_str_mv 2024-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/291132
Morcillo, Rafael Jorge León; Leal López, Jesús; Férez Gómez, Alberto; López Serrano, Lidia; Baroja Fernández, Edurne; et al.; RAPID ALKALINIZATION FACTOR 22 is a key modulator of the root hair growth responses to fungal ethylene emissions in Arabidopsis; American Society of Plant Biologist; Plant Physiology; 196; 4; 12-2024; 2890-2904
0032-0889
CONICET Digital
CONICET
url http://hdl.handle.net/11336/291132
identifier_str_mv Morcillo, Rafael Jorge León; Leal López, Jesús; Férez Gómez, Alberto; López Serrano, Lidia; Baroja Fernández, Edurne; et al.; RAPID ALKALINIZATION FACTOR 22 is a key modulator of the root hair growth responses to fungal ethylene emissions in Arabidopsis; American Society of Plant Biologist; Plant Physiology; 196; 4; 12-2024; 2890-2904
0032-0889
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/plphys/article/196/4/2890/7758768
info:eu-repo/semantics/altIdentifier/doi/10.1093/plphys/kiae484
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society of Plant Biologist
publisher.none.fl_str_mv American Society of Plant Biologist
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.265058

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