A self-amplifying mRNA vaccine expressing PRV gD induces robust immunity against virulent mutants

Autores
Tong, Ling; Zhang, Xin; Li, Huan-huan; Zheng, Ya-ting; Liu, Ya-mei; Guo, Rong-li; Damiani, Armando Mario; Chen, Sai-sai; Zhang, Chuan-jian; Duan. Rui; Mao, Shu-yu; Yu, Jia-li; Zhang, Qian-qian; Tao, Ruo-nan; Osterrieder, Nikolaus; Xia, Shu-hua; Wang, Ji-chun
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Pseudorabies virus (PRV) causes an acute febrile infectious disease of pigs. Since 2011, PRV variants have appeared and spread nationwide in China. mRNA vaccines present a safe alternative and can stimulate humoral and cellular immunity. We constructed a self-amplifying mRNA (saRNA) vaccine containing gD gene of a highly virulent PRV variant. In mice, the PRV saRNA gD vaccine induced high levels of neutralizing antibodies. In piglets, vaccination with the PRV saRNA gD vaccine at a dose of as little as 5 μg induced very strong humoral immunity and cellular immunity, providing full protection against challenge infection with very virulent PRV variants. Protection following vaccination with saRNA gD induced significantly stronger protection than attenuated live vaccines and inactivated vaccines. In addition, our results confirm that a PRV saRNA vaccine expressing only one antigen can induce excellent protection in weaned piglets. Hence, the PRV saRNA gD vaccine might be a very promising substitute for inactivated and live vaccines. Finally, we provide proof-of-concept for the development of self-amplifying mRNA vaccines for members of the Alphaherpesvirinae, including herpes simplex virus 1 and 2 as well as varicella zoster virus.
Fil: Tong, Ling. Jiangsu Academy of Agricultural Sciences; China. Jiangsu Coinnovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses; China. Center of Technology Innovation for Veterinary Biology; China
Fil: Zhang, Xin. Jiangsu Academy of Agricultural Sciences; China. Nanjing Agricultural University; China
Fil: Li, Huan-huan. Jiangsu Academy of Agricultural Sciences; China. Guangxi University; China
Fil: Zheng, Ya-ting. Jiangsu Academy of Agricultural Sciences; China. Jiangsu Coinnovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses; China. Center of Technology Innovation for Veterinary Biology; China
Fil: Liu, Ya-mei. Jiangsu Academy of Agricultural Sciences; China. Jiangsu Coinnovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses; China. Center of Technology Innovation for Veterinary Biology; China
Fil: Guo, Rong-li. Jiangsu Academy of Agricultural Sciences; China
Fil: Damiani, Armando Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de Cs.medicas. Area Quimica Biologica; Argentina
Fil: Chen, Sai-sai. Jiangsu Academy of Agricultural Sciences; China. Jiangsu Coinnovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses; China. Center of Technology Innovation for Veterinary Biology; China
Fil: Zhang, Chuan-jian. Jiangsu Academy of Agricultural Sciences; China. Jiangsu Coinnovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses; China. Center of Technology Innovation for Veterinary Biology; China
Fil: Duan. Rui. Jinfa Pharmaceutical; China
Fil: Mao, Shu-yu. Jinfa Pharmaceutical; China
Fil: Yu, Jia-li. Jinfa Pharmaceutical; China
Fil: Zhang, Qian-qian. Jinfa Pharmaceutical; China
Fil: Tao, Ruo-nan. Jinfa Pharmaceutical; China
Fil: Osterrieder, Nikolaus. Tierärztliche Hochschule Hannover; Alemania
Fil: Xia, Shu-hua. Jinfa Pharmaceutical; China
Fil: Wang, Ji-chun. Jiangsu Academy of Agricultural Sciences; China. Jiangsu Coinnovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses; China. Center of Technology Innovation for Veterinary Biology; China
Materia
PRV
mRNA
VACCINE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/291785
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/291785
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A self-amplifying mRNA vaccine expressing PRV gD induces robust immunity against virulent mutantsTong, LingZhang, XinLi, Huan-huanZheng, Ya-tingLiu, Ya-meiGuo, Rong-liDamiani, Armando MarioChen, Sai-saiZhang, Chuan-jianDuan. RuiMao, Shu-yuYu, Jia-liZhang, Qian-qianTao, Ruo-nanOsterrieder, NikolausXia, Shu-huaWang, Ji-chunPRVmRNAVACCINEhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Pseudorabies virus (PRV) causes an acute febrile infectious disease of pigs. Since 2011, PRV variants have appeared and spread nationwide in China. mRNA vaccines present a safe alternative and can stimulate humoral and cellular immunity. We constructed a self-amplifying mRNA (saRNA) vaccine containing gD gene of a highly virulent PRV variant. In mice, the PRV saRNA gD vaccine induced high levels of neutralizing antibodies. In piglets, vaccination with the PRV saRNA gD vaccine at a dose of as little as 5 μg induced very strong humoral immunity and cellular immunity, providing full protection against challenge infection with very virulent PRV variants. Protection following vaccination with saRNA gD induced significantly stronger protection than attenuated live vaccines and inactivated vaccines. In addition, our results confirm that a PRV saRNA vaccine expressing only one antigen can induce excellent protection in weaned piglets. Hence, the PRV saRNA gD vaccine might be a very promising substitute for inactivated and live vaccines. Finally, we provide proof-of-concept for the development of self-amplifying mRNA vaccines for members of the Alphaherpesvirinae, including herpes simplex virus 1 and 2 as well as varicella zoster virus.Fil: Tong, Ling. Jiangsu Academy of Agricultural Sciences; China. Jiangsu Coinnovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses; China. Center of Technology Innovation for Veterinary Biology; ChinaFil: Zhang, Xin. Jiangsu Academy of Agricultural Sciences; China. Nanjing Agricultural University; ChinaFil: Li, Huan-huan. Jiangsu Academy of Agricultural Sciences; China. Guangxi University; ChinaFil: Zheng, Ya-ting. Jiangsu Academy of Agricultural Sciences; China. Jiangsu Coinnovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses; China. Center of Technology Innovation for Veterinary Biology; ChinaFil: Liu, Ya-mei. Jiangsu Academy of Agricultural Sciences; China. Jiangsu Coinnovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses; China. Center of Technology Innovation for Veterinary Biology; ChinaFil: Guo, Rong-li. Jiangsu Academy of Agricultural Sciences; ChinaFil: Damiani, Armando Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de Cs.medicas. Area Quimica Biologica; ArgentinaFil: Chen, Sai-sai. Jiangsu Academy of Agricultural Sciences; China. Jiangsu Coinnovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses; China. Center of Technology Innovation for Veterinary Biology; ChinaFil: Zhang, Chuan-jian. Jiangsu Academy of Agricultural Sciences; China. Jiangsu Coinnovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses; China. Center of Technology Innovation for Veterinary Biology; ChinaFil: Duan. Rui. Jinfa Pharmaceutical; ChinaFil: Mao, Shu-yu. Jinfa Pharmaceutical; ChinaFil: Yu, Jia-li. Jinfa Pharmaceutical; ChinaFil: Zhang, Qian-qian. Jinfa Pharmaceutical; ChinaFil: Tao, Ruo-nan. Jinfa Pharmaceutical; ChinaFil: Osterrieder, Nikolaus. Tierärztliche Hochschule Hannover; AlemaniaFil: Xia, Shu-hua. Jinfa Pharmaceutical; ChinaFil: Wang, Ji-chun. Jiangsu Academy of Agricultural Sciences; China. Jiangsu Coinnovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses; China. Center of Technology Innovation for Veterinary Biology; ChinaNature Publishing Group2025-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/291785Tong, Ling; Zhang, Xin; Li, Huan-huan; Zheng, Ya-ting; Liu, Ya-mei; et al.; A self-amplifying mRNA vaccine expressing PRV gD induces robust immunity against virulent mutants; Nature Publishing Group; npj Vaccines; 10; 1; 8-2025; 1-122059-0105CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41541-025-01251-5info:eu-repo/semantics/altIdentifier/doi/10.1038/s41541-025-01251-5info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-08-25T15:31:45Zoai:ri.conicet.gov.ar:11336/291785instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-08-25 15:31:45.566CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A self-amplifying mRNA vaccine expressing PRV gD induces robust immunity against virulent mutants
title A self-amplifying mRNA vaccine expressing PRV gD induces robust immunity against virulent mutants
spellingShingle A self-amplifying mRNA vaccine expressing PRV gD induces robust immunity against virulent mutants
Tong, Ling
PRV
mRNA
VACCINE
title_short A self-amplifying mRNA vaccine expressing PRV gD induces robust immunity against virulent mutants
title_full A self-amplifying mRNA vaccine expressing PRV gD induces robust immunity against virulent mutants
title_fullStr A self-amplifying mRNA vaccine expressing PRV gD induces robust immunity against virulent mutants
title_full_unstemmed A self-amplifying mRNA vaccine expressing PRV gD induces robust immunity against virulent mutants
title_sort A self-amplifying mRNA vaccine expressing PRV gD induces robust immunity against virulent mutants
dc.creator.none.fl_str_mv Tong, Ling
Zhang, Xin
Li, Huan-huan
Zheng, Ya-ting
Liu, Ya-mei
Guo, Rong-li
Damiani, Armando Mario
Chen, Sai-sai
Zhang, Chuan-jian
Duan. Rui
Mao, Shu-yu
Yu, Jia-li
Zhang, Qian-qian
Tao, Ruo-nan
Osterrieder, Nikolaus
Xia, Shu-hua
Wang, Ji-chun
author Tong, Ling
author_facet Tong, Ling
Zhang, Xin
Li, Huan-huan
Zheng, Ya-ting
Liu, Ya-mei
Guo, Rong-li
Damiani, Armando Mario
Chen, Sai-sai
Zhang, Chuan-jian
Duan. Rui
Mao, Shu-yu
Yu, Jia-li
Zhang, Qian-qian
Tao, Ruo-nan
Osterrieder, Nikolaus
Xia, Shu-hua
Wang, Ji-chun
author_role author
author2 Zhang, Xin
Li, Huan-huan
Zheng, Ya-ting
Liu, Ya-mei
Guo, Rong-li
Damiani, Armando Mario
Chen, Sai-sai
Zhang, Chuan-jian
Duan. Rui
Mao, Shu-yu
Yu, Jia-li
Zhang, Qian-qian
Tao, Ruo-nan
Osterrieder, Nikolaus
Xia, Shu-hua
Wang, Ji-chun
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv PRV
mRNA
VACCINE
topic PRV
mRNA
VACCINE
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Pseudorabies virus (PRV) causes an acute febrile infectious disease of pigs. Since 2011, PRV variants have appeared and spread nationwide in China. mRNA vaccines present a safe alternative and can stimulate humoral and cellular immunity. We constructed a self-amplifying mRNA (saRNA) vaccine containing gD gene of a highly virulent PRV variant. In mice, the PRV saRNA gD vaccine induced high levels of neutralizing antibodies. In piglets, vaccination with the PRV saRNA gD vaccine at a dose of as little as 5 μg induced very strong humoral immunity and cellular immunity, providing full protection against challenge infection with very virulent PRV variants. Protection following vaccination with saRNA gD induced significantly stronger protection than attenuated live vaccines and inactivated vaccines. In addition, our results confirm that a PRV saRNA vaccine expressing only one antigen can induce excellent protection in weaned piglets. Hence, the PRV saRNA gD vaccine might be a very promising substitute for inactivated and live vaccines. Finally, we provide proof-of-concept for the development of self-amplifying mRNA vaccines for members of the Alphaherpesvirinae, including herpes simplex virus 1 and 2 as well as varicella zoster virus.
Fil: Tong, Ling. Jiangsu Academy of Agricultural Sciences; China. Jiangsu Coinnovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses; China. Center of Technology Innovation for Veterinary Biology; China
Fil: Zhang, Xin. Jiangsu Academy of Agricultural Sciences; China. Nanjing Agricultural University; China
Fil: Li, Huan-huan. Jiangsu Academy of Agricultural Sciences; China. Guangxi University; China
Fil: Zheng, Ya-ting. Jiangsu Academy of Agricultural Sciences; China. Jiangsu Coinnovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses; China. Center of Technology Innovation for Veterinary Biology; China
Fil: Liu, Ya-mei. Jiangsu Academy of Agricultural Sciences; China. Jiangsu Coinnovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses; China. Center of Technology Innovation for Veterinary Biology; China
Fil: Guo, Rong-li. Jiangsu Academy of Agricultural Sciences; China
Fil: Damiani, Armando Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de Cs.medicas. Area Quimica Biologica; Argentina
Fil: Chen, Sai-sai. Jiangsu Academy of Agricultural Sciences; China. Jiangsu Coinnovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses; China. Center of Technology Innovation for Veterinary Biology; China
Fil: Zhang, Chuan-jian. Jiangsu Academy of Agricultural Sciences; China. Jiangsu Coinnovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses; China. Center of Technology Innovation for Veterinary Biology; China
Fil: Duan. Rui. Jinfa Pharmaceutical; China
Fil: Mao, Shu-yu. Jinfa Pharmaceutical; China
Fil: Yu, Jia-li. Jinfa Pharmaceutical; China
Fil: Zhang, Qian-qian. Jinfa Pharmaceutical; China
Fil: Tao, Ruo-nan. Jinfa Pharmaceutical; China
Fil: Osterrieder, Nikolaus. Tierärztliche Hochschule Hannover; Alemania
Fil: Xia, Shu-hua. Jinfa Pharmaceutical; China
Fil: Wang, Ji-chun. Jiangsu Academy of Agricultural Sciences; China. Jiangsu Coinnovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses; China. Center of Technology Innovation for Veterinary Biology; China
description Pseudorabies virus (PRV) causes an acute febrile infectious disease of pigs. Since 2011, PRV variants have appeared and spread nationwide in China. mRNA vaccines present a safe alternative and can stimulate humoral and cellular immunity. We constructed a self-amplifying mRNA (saRNA) vaccine containing gD gene of a highly virulent PRV variant. In mice, the PRV saRNA gD vaccine induced high levels of neutralizing antibodies. In piglets, vaccination with the PRV saRNA gD vaccine at a dose of as little as 5 μg induced very strong humoral immunity and cellular immunity, providing full protection against challenge infection with very virulent PRV variants. Protection following vaccination with saRNA gD induced significantly stronger protection than attenuated live vaccines and inactivated vaccines. In addition, our results confirm that a PRV saRNA vaccine expressing only one antigen can induce excellent protection in weaned piglets. Hence, the PRV saRNA gD vaccine might be a very promising substitute for inactivated and live vaccines. Finally, we provide proof-of-concept for the development of self-amplifying mRNA vaccines for members of the Alphaherpesvirinae, including herpes simplex virus 1 and 2 as well as varicella zoster virus.
publishDate 2025
dc.date.none.fl_str_mv 2025-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/291785
Tong, Ling; Zhang, Xin; Li, Huan-huan; Zheng, Ya-ting; Liu, Ya-mei; et al.; A self-amplifying mRNA vaccine expressing PRV gD induces robust immunity against virulent mutants; Nature Publishing Group; npj Vaccines; 10; 1; 8-2025; 1-12
2059-0105
CONICET Digital
CONICET
url http://hdl.handle.net/11336/291785
identifier_str_mv Tong, Ling; Zhang, Xin; Li, Huan-huan; Zheng, Ya-ting; Liu, Ya-mei; et al.; A self-amplifying mRNA vaccine expressing PRV gD induces robust immunity against virulent mutants; Nature Publishing Group; npj Vaccines; 10; 1; 8-2025; 1-12
2059-0105
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41541-025-01251-5
info:eu-repo/semantics/altIdentifier/doi/10.1038/s41541-025-01251-5
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.24418

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