Performance of a crude enzymatic cocktail produced by Aspergillus niger LBM 321 in the recovery of bound phenolic compounds

Autores
Díaz, Gabriela Verónica; Coniglio, Romina Olga; Rodríguez, María Daniela; Bordaquievich, Mayra Florencia; Zapata, Pedro Dario; Fonseca, Maria Isabel
Año de publicación
2026
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Jabuticaba (Plinia cauliflora) processing generates substantial epicarp waste rich in phenolic compounds, which have antioxidant and health-promoting potential. To valorize the jabuticaba epicarps via a green biotechnological approach, we developed and optimized an enzyme extraction of phenolic compounds using a fungal cocktail produced by Aspergillus niger LBM 321. A central composite design was employed to evaluate the effects of temperature, pH, and time of extraction. The optimal conditions were 38.5 C, pH 5.34, and 10 h yielded 72.94 mg GAE 100 mL⁻¹. Under these conditions, enzymatic extraction outperformed alkaline hydrolysis (1 N NaOH, pH 9.5) and commercial enzyme extraction (Viscozyme L). UHPLC-MS/MS analysis revealed that ρ-coumaric and ferulic acids were the predominant phenolics in the fungal enzyme extracts, suggesting preferential liberation of ester-linked hydroxycinnamic acids from the cell wall matrix. FTIR spectroscopy presented enhanced C?O stretching bands after enzymatic treatment, indicating selective polysaccharide hydrolysis and release of phenolic compounds. This study demonstrates that A. niger LBM 321 enzymes offer an eco-friendly and cost-effective strategy for recovering high-value phenolics from jabuticaba epicarps. By converting waste into functional ingredients, this approach supports a circular-economy model and aligns with consumer demands for natural, sustainable food additives.
Fil: Díaz, Gabriela Verónica. Universidad Nacional de Misiones. Facultad de Cs.exactas Químicas y Naturales. Departamento de Bioquímica Clinica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Coniglio, Romina Olga. Universidad Nacional de Misiones. Facultad de Cs.exactas Químicas y Naturales. Departamento de Bioquímica Clinica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Rodríguez, María Daniela. Universidad Nacional de Misiones. Facultad de Cs.exactas Químicas y Naturales. Departamento de Bioquímica Clinica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Bordaquievich, Mayra Florencia. Universidad Nacional de Misiones. Facultad de Cs.exactas Químicas y Naturales. Departamento de Bioquímica Clinica; Argentina
Fil: Zapata, Pedro Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Cs.exactas Químicas y Naturales. Departamento de Bioquímica Clinica; Argentina
Fil: Fonseca, Maria Isabel. Universidad Nacional de Misiones. Facultad de Cs.exactas Químicas y Naturales. Departamento de Bioquímica Clinica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Materia
BIOACTIVE COMPOUNDS
JABUTICABA EPICARPS
ENZYME-ASSISTED EXTRACTION
ASPERGILLUS NIGER LBM 321
PHENOLIC COMPOUNDS
RESPONSE SURFACE METHODOLOGY
p-COUMARIC ACID
CIRCULAR ECONOMY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/290000
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/290000
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Performance of a crude enzymatic cocktail produced by Aspergillus niger LBM 321 in the recovery of bound phenolic compoundsDíaz, Gabriela VerónicaConiglio, Romina OlgaRodríguez, María DanielaBordaquievich, Mayra FlorenciaZapata, Pedro DarioFonseca, Maria IsabelBIOACTIVE COMPOUNDSJABUTICABA EPICARPSENZYME-ASSISTED EXTRACTIONASPERGILLUS NIGER LBM 321PHENOLIC COMPOUNDSRESPONSE SURFACE METHODOLOGYp-COUMARIC ACIDCIRCULAR ECONOMYhttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3Jabuticaba (Plinia cauliflora) processing generates substantial epicarp waste rich in phenolic compounds, which have antioxidant and health-promoting potential. To valorize the jabuticaba epicarps via a green biotechnological approach, we developed and optimized an enzyme extraction of phenolic compounds using a fungal cocktail produced by Aspergillus niger LBM 321. A central composite design was employed to evaluate the effects of temperature, pH, and time of extraction. The optimal conditions were 38.5 C, pH 5.34, and 10 h yielded 72.94 mg GAE 100 mL⁻¹. Under these conditions, enzymatic extraction outperformed alkaline hydrolysis (1 N NaOH, pH 9.5) and commercial enzyme extraction (Viscozyme L). UHPLC-MS/MS analysis revealed that ρ-coumaric and ferulic acids were the predominant phenolics in the fungal enzyme extracts, suggesting preferential liberation of ester-linked hydroxycinnamic acids from the cell wall matrix. FTIR spectroscopy presented enhanced C?O stretching bands after enzymatic treatment, indicating selective polysaccharide hydrolysis and release of phenolic compounds. This study demonstrates that A. niger LBM 321 enzymes offer an eco-friendly and cost-effective strategy for recovering high-value phenolics from jabuticaba epicarps. By converting waste into functional ingredients, this approach supports a circular-economy model and aligns with consumer demands for natural, sustainable food additives.Fil: Díaz, Gabriela Verónica. Universidad Nacional de Misiones. Facultad de Cs.exactas Químicas y Naturales. Departamento de Bioquímica Clinica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaFil: Coniglio, Romina Olga. Universidad Nacional de Misiones. Facultad de Cs.exactas Químicas y Naturales. Departamento de Bioquímica Clinica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaFil: Rodríguez, María Daniela. Universidad Nacional de Misiones. Facultad de Cs.exactas Químicas y Naturales. Departamento de Bioquímica Clinica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaFil: Bordaquievich, Mayra Florencia. Universidad Nacional de Misiones. Facultad de Cs.exactas Químicas y Naturales. Departamento de Bioquímica Clinica; ArgentinaFil: Zapata, Pedro Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Cs.exactas Químicas y Naturales. Departamento de Bioquímica Clinica; ArgentinaFil: Fonseca, Maria Isabel. Universidad Nacional de Misiones. Facultad de Cs.exactas Químicas y Naturales. Departamento de Bioquímica Clinica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaElsevier2026-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/290000Díaz, Gabriela Verónica; Coniglio, Romina Olga; Rodríguez, María Daniela; Bordaquievich, Mayra Florencia; Zapata, Pedro Dario; et al.; Performance of a crude enzymatic cocktail produced by Aspergillus niger LBM 321 in the recovery of bound phenolic compounds; Elsevier; Food Chemistry Advances; 10; 1-2026; 1-82772-753XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S2772753X26000043?ref=pdf_download&fr=RR-2&rr=9ccc964ceef6ba6binfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.focha.2026.101221info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-08-25T14:52:46Zoai:ri.conicet.gov.ar:11336/290000instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-08-25 14:52:47.378CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Performance of a crude enzymatic cocktail produced by Aspergillus niger LBM 321 in the recovery of bound phenolic compounds
title Performance of a crude enzymatic cocktail produced by Aspergillus niger LBM 321 in the recovery of bound phenolic compounds
spellingShingle Performance of a crude enzymatic cocktail produced by Aspergillus niger LBM 321 in the recovery of bound phenolic compounds
Díaz, Gabriela Verónica
BIOACTIVE COMPOUNDS
JABUTICABA EPICARPS
ENZYME-ASSISTED EXTRACTION
ASPERGILLUS NIGER LBM 321
PHENOLIC COMPOUNDS
RESPONSE SURFACE METHODOLOGY
p-COUMARIC ACID
CIRCULAR ECONOMY
title_short Performance of a crude enzymatic cocktail produced by Aspergillus niger LBM 321 in the recovery of bound phenolic compounds
title_full Performance of a crude enzymatic cocktail produced by Aspergillus niger LBM 321 in the recovery of bound phenolic compounds
title_fullStr Performance of a crude enzymatic cocktail produced by Aspergillus niger LBM 321 in the recovery of bound phenolic compounds
title_full_unstemmed Performance of a crude enzymatic cocktail produced by Aspergillus niger LBM 321 in the recovery of bound phenolic compounds
title_sort Performance of a crude enzymatic cocktail produced by Aspergillus niger LBM 321 in the recovery of bound phenolic compounds
dc.creator.none.fl_str_mv Díaz, Gabriela Verónica
Coniglio, Romina Olga
Rodríguez, María Daniela
Bordaquievich, Mayra Florencia
Zapata, Pedro Dario
Fonseca, Maria Isabel
author Díaz, Gabriela Verónica
author_facet Díaz, Gabriela Verónica
Coniglio, Romina Olga
Rodríguez, María Daniela
Bordaquievich, Mayra Florencia
Zapata, Pedro Dario
Fonseca, Maria Isabel
author_role author
author2 Coniglio, Romina Olga
Rodríguez, María Daniela
Bordaquievich, Mayra Florencia
Zapata, Pedro Dario
Fonseca, Maria Isabel
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv BIOACTIVE COMPOUNDS
JABUTICABA EPICARPS
ENZYME-ASSISTED EXTRACTION
ASPERGILLUS NIGER LBM 321
PHENOLIC COMPOUNDS
RESPONSE SURFACE METHODOLOGY
p-COUMARIC ACID
CIRCULAR ECONOMY
topic BIOACTIVE COMPOUNDS
JABUTICABA EPICARPS
ENZYME-ASSISTED EXTRACTION
ASPERGILLUS NIGER LBM 321
PHENOLIC COMPOUNDS
RESPONSE SURFACE METHODOLOGY
p-COUMARIC ACID
CIRCULAR ECONOMY
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Jabuticaba (Plinia cauliflora) processing generates substantial epicarp waste rich in phenolic compounds, which have antioxidant and health-promoting potential. To valorize the jabuticaba epicarps via a green biotechnological approach, we developed and optimized an enzyme extraction of phenolic compounds using a fungal cocktail produced by Aspergillus niger LBM 321. A central composite design was employed to evaluate the effects of temperature, pH, and time of extraction. The optimal conditions were 38.5 C, pH 5.34, and 10 h yielded 72.94 mg GAE 100 mL⁻¹. Under these conditions, enzymatic extraction outperformed alkaline hydrolysis (1 N NaOH, pH 9.5) and commercial enzyme extraction (Viscozyme L). UHPLC-MS/MS analysis revealed that ρ-coumaric and ferulic acids were the predominant phenolics in the fungal enzyme extracts, suggesting preferential liberation of ester-linked hydroxycinnamic acids from the cell wall matrix. FTIR spectroscopy presented enhanced C?O stretching bands after enzymatic treatment, indicating selective polysaccharide hydrolysis and release of phenolic compounds. This study demonstrates that A. niger LBM 321 enzymes offer an eco-friendly and cost-effective strategy for recovering high-value phenolics from jabuticaba epicarps. By converting waste into functional ingredients, this approach supports a circular-economy model and aligns with consumer demands for natural, sustainable food additives.
Fil: Díaz, Gabriela Verónica. Universidad Nacional de Misiones. Facultad de Cs.exactas Químicas y Naturales. Departamento de Bioquímica Clinica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Coniglio, Romina Olga. Universidad Nacional de Misiones. Facultad de Cs.exactas Químicas y Naturales. Departamento de Bioquímica Clinica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Rodríguez, María Daniela. Universidad Nacional de Misiones. Facultad de Cs.exactas Químicas y Naturales. Departamento de Bioquímica Clinica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Bordaquievich, Mayra Florencia. Universidad Nacional de Misiones. Facultad de Cs.exactas Químicas y Naturales. Departamento de Bioquímica Clinica; Argentina
Fil: Zapata, Pedro Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Cs.exactas Químicas y Naturales. Departamento de Bioquímica Clinica; Argentina
Fil: Fonseca, Maria Isabel. Universidad Nacional de Misiones. Facultad de Cs.exactas Químicas y Naturales. Departamento de Bioquímica Clinica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
description Jabuticaba (Plinia cauliflora) processing generates substantial epicarp waste rich in phenolic compounds, which have antioxidant and health-promoting potential. To valorize the jabuticaba epicarps via a green biotechnological approach, we developed and optimized an enzyme extraction of phenolic compounds using a fungal cocktail produced by Aspergillus niger LBM 321. A central composite design was employed to evaluate the effects of temperature, pH, and time of extraction. The optimal conditions were 38.5 C, pH 5.34, and 10 h yielded 72.94 mg GAE 100 mL⁻¹. Under these conditions, enzymatic extraction outperformed alkaline hydrolysis (1 N NaOH, pH 9.5) and commercial enzyme extraction (Viscozyme L). UHPLC-MS/MS analysis revealed that ρ-coumaric and ferulic acids were the predominant phenolics in the fungal enzyme extracts, suggesting preferential liberation of ester-linked hydroxycinnamic acids from the cell wall matrix. FTIR spectroscopy presented enhanced C?O stretching bands after enzymatic treatment, indicating selective polysaccharide hydrolysis and release of phenolic compounds. This study demonstrates that A. niger LBM 321 enzymes offer an eco-friendly and cost-effective strategy for recovering high-value phenolics from jabuticaba epicarps. By converting waste into functional ingredients, this approach supports a circular-economy model and aligns with consumer demands for natural, sustainable food additives.
publishDate 2026
dc.date.none.fl_str_mv 2026-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/290000
Díaz, Gabriela Verónica; Coniglio, Romina Olga; Rodríguez, María Daniela; Bordaquievich, Mayra Florencia; Zapata, Pedro Dario; et al.; Performance of a crude enzymatic cocktail produced by Aspergillus niger LBM 321 in the recovery of bound phenolic compounds; Elsevier; Food Chemistry Advances; 10; 1-2026; 1-8
2772-753X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/290000
identifier_str_mv Díaz, Gabriela Verónica; Coniglio, Romina Olga; Rodríguez, María Daniela; Bordaquievich, Mayra Florencia; Zapata, Pedro Dario; et al.; Performance of a crude enzymatic cocktail produced by Aspergillus niger LBM 321 in the recovery of bound phenolic compounds; Elsevier; Food Chemistry Advances; 10; 1-2026; 1-8
2772-753X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S2772753X26000043?ref=pdf_download&fr=RR-2&rr=9ccc964ceef6ba6b
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.focha.2026.101221
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.265058

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