Signs of a phyllospheric lifestyle in the genome of the stress-tolerantstrain Azospirillum brasilense Az19

Autores
Garcia, Julia Elena; Labarthe, Maria Mercedes; Pagnussat, Luciana Anabella; Amenta, Melina Beatriz; Creus, Ceciclia Monica; Maroniche, Guillermo Andres
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Azospirillum brasilense Az19 is a plant-beneficial bacterium capable of protecting plants from the negativeeffects of drought. The objective of this study was to determine and analyze the genomic sequence of strainAz19 as a means of identifying putative stress-adaptation mechanisms. A high-quality draft genome of ca.7 Mb with a predicted coding potential of 6710 genes was obtained. Phylogenomic analyses confirmedthat Az19 belongs to the brasilense clade and is closely related to strains Az39 and REC3. Functionalgenomics revealed that the denitrification pathway of Az19 is incomplete, which was in agreement witha reduced growth on nitrate under low O2concentrations. Putative genes of the general stress responseand oxidative stress-tolerance, as well as synthesis of exopolysaccharides, carotenoids, polyamines andseveral osmolytes, were detected. An additional poly-beta-hydroxybutyrate (PHB) synthase coding genewas found in Az19 genome, but the accumulation of PHB did not increase under salinity. The detection ofexclusive genes related to DNA repair led to discover that strain Az19 also has improved UV-tolerance,both in vitro and in planta. Finally, the analysis revealed the presence of multiple kaiC-like genes, whichcould be involved in stress-tolerance and, possibly, light responsiveness. Although A. brasilense has beena model for the study of beneficial plant-associated rhizobacteria, the evidence collected in this currentstudy suggests, for the first time in this bacterial group, an unexpected possibility of adaptation to thephyllosphere.
Instituto de Microbiología y Zoología Agrícola (IMYZA)
Fil: Garcia, Julia Elena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina
Fil: Labarthe, María Mercedes. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; Argentina
Fil: Pagnussat, Luciana Anabella. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; Argentina
Fil: Pagnussat, Luciana Anabella. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Amenta, Melina Beatriz. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; Argentina
Fil: Creus, Cecilia Mónica. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; Argentina
Fil: Maroniche, Guillermo Andrés. Universidad de Buenos Aires (UBA). Facultad de Farmacia y Bioquímica; Argentina
Fil: Maroniche, Guillermo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fuente
Systematic And Applied Microbiology 43 (6) : 126130 (2020)
Materia
Azospirillum
Azospirillum brasilense
Resistencia a la Sequía
Análisis Filogenético
Drought Resistance
Phylogenetic Analysis
Secuencia Genómica
Bacterias Benéficas
Genome Sequence
Beneficial Bacteria
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/27294
Acceder
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oai_identifier_str oai:localhost:20.500.12123/27294
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Signs of a phyllospheric lifestyle in the genome of the stress-tolerantstrain Azospirillum brasilense Az19Garcia, Julia ElenaLabarthe, Maria MercedesPagnussat, Luciana AnabellaAmenta, Melina BeatrizCreus, Ceciclia MonicaMaroniche, Guillermo AndresAzospirillumAzospirillum brasilenseResistencia a la SequíaAnálisis FilogenéticoDrought ResistancePhylogenetic AnalysisSecuencia GenómicaBacterias BenéficasGenome SequenceBeneficial BacteriaAzospirillum brasilense Az19 is a plant-beneficial bacterium capable of protecting plants from the negativeeffects of drought. The objective of this study was to determine and analyze the genomic sequence of strainAz19 as a means of identifying putative stress-adaptation mechanisms. A high-quality draft genome of ca.7 Mb with a predicted coding potential of 6710 genes was obtained. Phylogenomic analyses confirmedthat Az19 belongs to the brasilense clade and is closely related to strains Az39 and REC3. Functionalgenomics revealed that the denitrification pathway of Az19 is incomplete, which was in agreement witha reduced growth on nitrate under low O2concentrations. Putative genes of the general stress responseand oxidative stress-tolerance, as well as synthesis of exopolysaccharides, carotenoids, polyamines andseveral osmolytes, were detected. An additional poly-beta-hydroxybutyrate (PHB) synthase coding genewas found in Az19 genome, but the accumulation of PHB did not increase under salinity. The detection ofexclusive genes related to DNA repair led to discover that strain Az19 also has improved UV-tolerance,both in vitro and in planta. Finally, the analysis revealed the presence of multiple kaiC-like genes, whichcould be involved in stress-tolerance and, possibly, light responsiveness. Although A. brasilense has beena model for the study of beneficial plant-associated rhizobacteria, the evidence collected in this currentstudy suggests, for the first time in this bacterial group, an unexpected possibility of adaptation to thephyllosphere.Instituto de Microbiología y Zoología Agrícola (IMYZA)Fil: Garcia, Julia Elena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); ArgentinaFil: Labarthe, María Mercedes. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; ArgentinaFil: Pagnussat, Luciana Anabella. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; ArgentinaFil: Pagnussat, Luciana Anabella. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); ArgentinaFil: Amenta, Melina Beatriz. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; ArgentinaFil: Creus, Cecilia Mónica. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; ArgentinaFil: Maroniche, Guillermo Andrés. Universidad de Buenos Aires (UBA). Facultad de Farmacia y Bioquímica; ArgentinaFil: Maroniche, Guillermo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); ArgentinaElsevier2026-08-05T12:20:13Z2026-08-05T12:20:13Z2020-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/27294https://www.sciencedirect.com/science/article/pii/S07232020203008500723-2020https://doi.org/10.1016/j.syapm.2020.126130Systematic And Applied Microbiology 43 (6) : 126130 (2020)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-09-24T11:43:39Zoai:localhost:20.500.12123/27294instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-09-24 11:43:40.285INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Signs of a phyllospheric lifestyle in the genome of the stress-tolerantstrain Azospirillum brasilense Az19
title Signs of a phyllospheric lifestyle in the genome of the stress-tolerantstrain Azospirillum brasilense Az19
spellingShingle Signs of a phyllospheric lifestyle in the genome of the stress-tolerantstrain Azospirillum brasilense Az19
Garcia, Julia Elena
Azospirillum
Azospirillum brasilense
Resistencia a la Sequía
Análisis Filogenético
Drought Resistance
Phylogenetic Analysis
Secuencia Genómica
Bacterias Benéficas
Genome Sequence
Beneficial Bacteria
title_short Signs of a phyllospheric lifestyle in the genome of the stress-tolerantstrain Azospirillum brasilense Az19
title_full Signs of a phyllospheric lifestyle in the genome of the stress-tolerantstrain Azospirillum brasilense Az19
title_fullStr Signs of a phyllospheric lifestyle in the genome of the stress-tolerantstrain Azospirillum brasilense Az19
title_full_unstemmed Signs of a phyllospheric lifestyle in the genome of the stress-tolerantstrain Azospirillum brasilense Az19
title_sort Signs of a phyllospheric lifestyle in the genome of the stress-tolerantstrain Azospirillum brasilense Az19
dc.creator.none.fl_str_mv Garcia, Julia Elena
Labarthe, Maria Mercedes
Pagnussat, Luciana Anabella
Amenta, Melina Beatriz
Creus, Ceciclia Monica
Maroniche, Guillermo Andres
author Garcia, Julia Elena
author_facet Garcia, Julia Elena
Labarthe, Maria Mercedes
Pagnussat, Luciana Anabella
Amenta, Melina Beatriz
Creus, Ceciclia Monica
Maroniche, Guillermo Andres
author_role author
author2 Labarthe, Maria Mercedes
Pagnussat, Luciana Anabella
Amenta, Melina Beatriz
Creus, Ceciclia Monica
Maroniche, Guillermo Andres
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Azospirillum
Azospirillum brasilense
Resistencia a la Sequía
Análisis Filogenético
Drought Resistance
Phylogenetic Analysis
Secuencia Genómica
Bacterias Benéficas
Genome Sequence
Beneficial Bacteria
topic Azospirillum
Azospirillum brasilense
Resistencia a la Sequía
Análisis Filogenético
Drought Resistance
Phylogenetic Analysis
Secuencia Genómica
Bacterias Benéficas
Genome Sequence
Beneficial Bacteria
dc.description.none.fl_txt_mv Azospirillum brasilense Az19 is a plant-beneficial bacterium capable of protecting plants from the negativeeffects of drought. The objective of this study was to determine and analyze the genomic sequence of strainAz19 as a means of identifying putative stress-adaptation mechanisms. A high-quality draft genome of ca.7 Mb with a predicted coding potential of 6710 genes was obtained. Phylogenomic analyses confirmedthat Az19 belongs to the brasilense clade and is closely related to strains Az39 and REC3. Functionalgenomics revealed that the denitrification pathway of Az19 is incomplete, which was in agreement witha reduced growth on nitrate under low O2concentrations. Putative genes of the general stress responseand oxidative stress-tolerance, as well as synthesis of exopolysaccharides, carotenoids, polyamines andseveral osmolytes, were detected. An additional poly-beta-hydroxybutyrate (PHB) synthase coding genewas found in Az19 genome, but the accumulation of PHB did not increase under salinity. The detection ofexclusive genes related to DNA repair led to discover that strain Az19 also has improved UV-tolerance,both in vitro and in planta. Finally, the analysis revealed the presence of multiple kaiC-like genes, whichcould be involved in stress-tolerance and, possibly, light responsiveness. Although A. brasilense has beena model for the study of beneficial plant-associated rhizobacteria, the evidence collected in this currentstudy suggests, for the first time in this bacterial group, an unexpected possibility of adaptation to thephyllosphere.
Instituto de Microbiología y Zoología Agrícola (IMYZA)
Fil: Garcia, Julia Elena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina
Fil: Labarthe, María Mercedes. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; Argentina
Fil: Pagnussat, Luciana Anabella. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; Argentina
Fil: Pagnussat, Luciana Anabella. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Amenta, Melina Beatriz. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; Argentina
Fil: Creus, Cecilia Mónica. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; Argentina
Fil: Maroniche, Guillermo Andrés. Universidad de Buenos Aires (UBA). Facultad de Farmacia y Bioquímica; Argentina
Fil: Maroniche, Guillermo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
description Azospirillum brasilense Az19 is a plant-beneficial bacterium capable of protecting plants from the negativeeffects of drought. The objective of this study was to determine and analyze the genomic sequence of strainAz19 as a means of identifying putative stress-adaptation mechanisms. A high-quality draft genome of ca.7 Mb with a predicted coding potential of 6710 genes was obtained. Phylogenomic analyses confirmedthat Az19 belongs to the brasilense clade and is closely related to strains Az39 and REC3. Functionalgenomics revealed that the denitrification pathway of Az19 is incomplete, which was in agreement witha reduced growth on nitrate under low O2concentrations. Putative genes of the general stress responseand oxidative stress-tolerance, as well as synthesis of exopolysaccharides, carotenoids, polyamines andseveral osmolytes, were detected. An additional poly-beta-hydroxybutyrate (PHB) synthase coding genewas found in Az19 genome, but the accumulation of PHB did not increase under salinity. The detection ofexclusive genes related to DNA repair led to discover that strain Az19 also has improved UV-tolerance,both in vitro and in planta. Finally, the analysis revealed the presence of multiple kaiC-like genes, whichcould be involved in stress-tolerance and, possibly, light responsiveness. Although A. brasilense has beena model for the study of beneficial plant-associated rhizobacteria, the evidence collected in this currentstudy suggests, for the first time in this bacterial group, an unexpected possibility of adaptation to thephyllosphere.
publishDate 2020
dc.date.none.fl_str_mv 2020-07
2026-08-05T12:20:13Z
2026-08-05T12:20:13Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/27294
https://www.sciencedirect.com/science/article/pii/S0723202020300850
0723-2020
https://doi.org/10.1016/j.syapm.2020.126130
url http://hdl.handle.net/20.500.12123/27294
https://www.sciencedirect.com/science/article/pii/S0723202020300850
https://doi.org/10.1016/j.syapm.2020.126130
identifier_str_mv 0723-2020
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Systematic And Applied Microbiology 43 (6) : 126130 (2020)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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