Signs of a phyllospheric lifestyle in the genome of the stress-tolerant strain Azospirillum brasilense Az19

Autores
Garcia, Julia Elena; Labarthe, María Mercedes; Pagnussat, Luciana Anabella; Amenta, Melina Beatriz; Creus, Cecilia Mónica; Maroniche, Guillermo Andrés
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Azospirillum brasilense Az19 is a plant-beneficial bacterium capable of protecting plants from the negative effects of drought. The objective of this study was to determine and analyze the genomic sequence of strain Az19 as a means of identifying putative stress-adaptation mechanisms. A high-quality draft genome of ca. 7 Mb with a predicted coding potential of 6710 genes was obtained. Phylogenomic analyses confirmed that Az19 belongs to the brasilense clade and is closely related to strains Az39 and REC3. Functional genomics revealed that the denitrification pathway of Az19 is incomplete, which was in agreement with a reduced growth on nitrate under low O2 concentrations. Putative genes of the general stress response and oxidative stress-tolerance, as well as synthesis of exopolysaccharides, carotenoids, polyamines and several osmolytes, were detected. An additional poly-beta-hydroxybutyrate (PHB) synthase coding gene was found in Az19 genome, but the accumulation of PHB did not increase under salinity. The detection of exclusive genes related to DNA repair led to discover that strain Az19 also has improved UV-tolerance, both in vitro and in planta. Finally, the analysis revealed the presence of multiple kaiC-like genes, which could be involved in stress-tolerance and, possibly, light responsiveness. Although A. brasilense has been a model for the study of beneficial plant-associated rhizobacteria, the evidence collected in this current study suggests, for the first time in this bacterial group, an unexpected possibility of adaptation to the phyllosphere.
Instituto de Microbiología y Zoología Agrícola (IMYZA)
Fil: Garcia, Julia Elena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Labarthe, María Mercedes. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Pagnussat, Luciana Anabella. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Pagnussat, Luciana Anabella. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Amenta, Melina Beatriz. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Creus, Cecilia Mónica. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Maroniche, Guillermo Andrés. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Maroniche, Guillermo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Systematic and applied microbiology 43 (6) : 126130. (Noviembre 2020)
Materia
Phyllosphere
Azospirillum Brasilense
Ultraviolet Radiation
Phylogenetic Analysis
Strains
Radiación Ultravioleta
Filosfera
Análisis Filogenético
Cepas
Phylogenomics
Incomplete Denitrification
PHB
UV-tolerance
kaiC
Strain Az19
Cepa Az19
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/13916
Acceder
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oai_identifier_str oai:localhost:20.500.12123/13916
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Signs of a phyllospheric lifestyle in the genome of the stress-tolerant strain Azospirillum brasilense Az19Garcia, Julia ElenaLabarthe, María MercedesPagnussat, Luciana AnabellaAmenta, Melina BeatrizCreus, Cecilia MónicaMaroniche, Guillermo AndrésPhyllosphereAzospirillum BrasilenseUltraviolet RadiationPhylogenetic AnalysisStrainsRadiación UltravioletaFilosferaAnálisis FilogenéticoCepasPhylogenomicsIncomplete DenitrificationPHBUV-tolerancekaiCStrain Az19Cepa Az19Azospirillum brasilense Az19 is a plant-beneficial bacterium capable of protecting plants from the negative effects of drought. The objective of this study was to determine and analyze the genomic sequence of strain Az19 as a means of identifying putative stress-adaptation mechanisms. A high-quality draft genome of ca. 7 Mb with a predicted coding potential of 6710 genes was obtained. Phylogenomic analyses confirmed that Az19 belongs to the brasilense clade and is closely related to strains Az39 and REC3. Functional genomics revealed that the denitrification pathway of Az19 is incomplete, which was in agreement with a reduced growth on nitrate under low O2 concentrations. Putative genes of the general stress response and oxidative stress-tolerance, as well as synthesis of exopolysaccharides, carotenoids, polyamines and several osmolytes, were detected. An additional poly-beta-hydroxybutyrate (PHB) synthase coding gene was found in Az19 genome, but the accumulation of PHB did not increase under salinity. The detection of exclusive genes related to DNA repair led to discover that strain Az19 also has improved UV-tolerance, both in vitro and in planta. Finally, the analysis revealed the presence of multiple kaiC-like genes, which could be involved in stress-tolerance and, possibly, light responsiveness. Although A. brasilense has been a model for the study of beneficial plant-associated rhizobacteria, the evidence collected in this current study suggests, for the first time in this bacterial group, an unexpected possibility of adaptation to the phyllosphere.Instituto de Microbiología y Zoología Agrícola (IMYZA)Fil: Garcia, Julia Elena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; ArgentinaFil: Labarthe, María Mercedes. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; ArgentinaFil: Pagnussat, Luciana Anabella. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; ArgentinaFil: Pagnussat, Luciana Anabella. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Amenta, Melina Beatriz. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; ArgentinaFil: Creus, Cecilia Mónica. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; ArgentinaFil: Maroniche, Guillermo Andrés. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; ArgentinaFil: Maroniche, Guillermo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2023-01-17T16:21:29Z2023-01-17T16:21:29Z2020-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/13916https://www.sciencedirect.com/science/article/abs/pii/S0723202020300850Systematic and applied microbiology 43 (6) : 126130. (Noviembre 2020)0723-20201618-0984https://doi.org/10.1016/j.syapm.2020.126130Systematic and applied microbiology 43 (6) : 126130. (Noviembre 2020)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2019-PD-E4-I069-001/2019-PD-E4-I069-001/AR./Bioprospección y caracterización de microorganismos benéficos para la protección y producción vegetalinfo:eu-repograntAgreement/INTA/2019-PD-E2-I037-002/2019-PD-E2-I037-002/AR./Biodiversidad edáfica: componente clave para una gestión integral y sustentable del recurso sueloinfo:eu-repo/semantics/restrictedAccess2026-09-24T11:38:23Zoai:localhost:20.500.12123/13916instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-09-24 11:38:25.464INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Signs of a phyllospheric lifestyle in the genome of the stress-tolerant strain Azospirillum brasilense Az19
title Signs of a phyllospheric lifestyle in the genome of the stress-tolerant strain Azospirillum brasilense Az19
spellingShingle Signs of a phyllospheric lifestyle in the genome of the stress-tolerant strain Azospirillum brasilense Az19
Garcia, Julia Elena
Phyllosphere
Azospirillum Brasilense
Ultraviolet Radiation
Phylogenetic Analysis
Strains
Radiación Ultravioleta
Filosfera
Análisis Filogenético
Cepas
Phylogenomics
Incomplete Denitrification
PHB
UV-tolerance
kaiC
Strain Az19
Cepa Az19
title_short Signs of a phyllospheric lifestyle in the genome of the stress-tolerant strain Azospirillum brasilense Az19
title_full Signs of a phyllospheric lifestyle in the genome of the stress-tolerant strain Azospirillum brasilense Az19
title_fullStr Signs of a phyllospheric lifestyle in the genome of the stress-tolerant strain Azospirillum brasilense Az19
title_full_unstemmed Signs of a phyllospheric lifestyle in the genome of the stress-tolerant strain Azospirillum brasilense Az19
title_sort Signs of a phyllospheric lifestyle in the genome of the stress-tolerant strain Azospirillum brasilense Az19
dc.creator.none.fl_str_mv Garcia, Julia Elena
Labarthe, María Mercedes
Pagnussat, Luciana Anabella
Amenta, Melina Beatriz
Creus, Cecilia Mónica
Maroniche, Guillermo Andrés
author Garcia, Julia Elena
author_facet Garcia, Julia Elena
Labarthe, María Mercedes
Pagnussat, Luciana Anabella
Amenta, Melina Beatriz
Creus, Cecilia Mónica
Maroniche, Guillermo Andrés
author_role author
author2 Labarthe, María Mercedes
Pagnussat, Luciana Anabella
Amenta, Melina Beatriz
Creus, Cecilia Mónica
Maroniche, Guillermo Andrés
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Phyllosphere
Azospirillum Brasilense
Ultraviolet Radiation
Phylogenetic Analysis
Strains
Radiación Ultravioleta
Filosfera
Análisis Filogenético
Cepas
Phylogenomics
Incomplete Denitrification
PHB
UV-tolerance
kaiC
Strain Az19
Cepa Az19
topic Phyllosphere
Azospirillum Brasilense
Ultraviolet Radiation
Phylogenetic Analysis
Strains
Radiación Ultravioleta
Filosfera
Análisis Filogenético
Cepas
Phylogenomics
Incomplete Denitrification
PHB
UV-tolerance
kaiC
Strain Az19
Cepa Az19
dc.description.none.fl_txt_mv Azospirillum brasilense Az19 is a plant-beneficial bacterium capable of protecting plants from the negative effects of drought. The objective of this study was to determine and analyze the genomic sequence of strain Az19 as a means of identifying putative stress-adaptation mechanisms. A high-quality draft genome of ca. 7 Mb with a predicted coding potential of 6710 genes was obtained. Phylogenomic analyses confirmed that Az19 belongs to the brasilense clade and is closely related to strains Az39 and REC3. Functional genomics revealed that the denitrification pathway of Az19 is incomplete, which was in agreement with a reduced growth on nitrate under low O2 concentrations. Putative genes of the general stress response and oxidative stress-tolerance, as well as synthesis of exopolysaccharides, carotenoids, polyamines and several osmolytes, were detected. An additional poly-beta-hydroxybutyrate (PHB) synthase coding gene was found in Az19 genome, but the accumulation of PHB did not increase under salinity. The detection of exclusive genes related to DNA repair led to discover that strain Az19 also has improved UV-tolerance, both in vitro and in planta. Finally, the analysis revealed the presence of multiple kaiC-like genes, which could be involved in stress-tolerance and, possibly, light responsiveness. Although A. brasilense has been a model for the study of beneficial plant-associated rhizobacteria, the evidence collected in this current study suggests, for the first time in this bacterial group, an unexpected possibility of adaptation to the phyllosphere.
Instituto de Microbiología y Zoología Agrícola (IMYZA)
Fil: Garcia, Julia Elena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Labarthe, María Mercedes. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Pagnussat, Luciana Anabella. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Pagnussat, Luciana Anabella. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Amenta, Melina Beatriz. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Creus, Cecilia Mónica. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Maroniche, Guillermo Andrés. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Maroniche, Guillermo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Azospirillum brasilense Az19 is a plant-beneficial bacterium capable of protecting plants from the negative effects of drought. The objective of this study was to determine and analyze the genomic sequence of strain Az19 as a means of identifying putative stress-adaptation mechanisms. A high-quality draft genome of ca. 7 Mb with a predicted coding potential of 6710 genes was obtained. Phylogenomic analyses confirmed that Az19 belongs to the brasilense clade and is closely related to strains Az39 and REC3. Functional genomics revealed that the denitrification pathway of Az19 is incomplete, which was in agreement with a reduced growth on nitrate under low O2 concentrations. Putative genes of the general stress response and oxidative stress-tolerance, as well as synthesis of exopolysaccharides, carotenoids, polyamines and several osmolytes, were detected. An additional poly-beta-hydroxybutyrate (PHB) synthase coding gene was found in Az19 genome, but the accumulation of PHB did not increase under salinity. The detection of exclusive genes related to DNA repair led to discover that strain Az19 also has improved UV-tolerance, both in vitro and in planta. Finally, the analysis revealed the presence of multiple kaiC-like genes, which could be involved in stress-tolerance and, possibly, light responsiveness. Although A. brasilense has been a model for the study of beneficial plant-associated rhizobacteria, the evidence collected in this current study suggests, for the first time in this bacterial group, an unexpected possibility of adaptation to the phyllosphere.
publishDate 2020
dc.date.none.fl_str_mv 2020-11
2023-01-17T16:21:29Z
2023-01-17T16:21:29Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/13916
https://www.sciencedirect.com/science/article/abs/pii/S0723202020300850
Systematic and applied microbiology 43 (6) : 126130. (Noviembre 2020)
0723-2020
1618-0984
https://doi.org/10.1016/j.syapm.2020.126130
url http://hdl.handle.net/20.500.12123/13916
https://www.sciencedirect.com/science/article/abs/pii/S0723202020300850
https://doi.org/10.1016/j.syapm.2020.126130
identifier_str_mv Systematic and applied microbiology 43 (6) : 126130. (Noviembre 2020)
0723-2020
1618-0984
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2019-PD-E4-I069-001/2019-PD-E4-I069-001/AR./Bioprospección y caracterización de microorganismos benéficos para la protección y producción vegetal
info:eu-repograntAgreement/INTA/2019-PD-E2-I037-002/2019-PD-E2-I037-002/AR./Biodiversidad edáfica: componente clave para una gestión integral y sustentable del recurso suelo
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Systematic and applied microbiology 43 (6) : 126130. (Noviembre 2020)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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