Feeding increasing amounts of Asparagopsis armata decreases enteric methane yield without compromising production in lactating dairy cows

Autores
Armand, Ezequiel; Salloum, María Soraya; Gimenez, Ruben Alejandro; Di Lorenzo, Nicolas; De León, Marcelo; Zenobi, Marcos Gonzalo
Año de publicación
2026
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Enteric methane (CH4) is the primary greenhouse gas emitted by ruminants. The red seaweed Asparagopsis armata (AA) effectively mitigates CH4, though optimal inclusion rates remain uncertain. The objective was to evaluate the dose–response effects of increasing AA inclusion levels on CH4 emissions, animal performance, feeding behavior, and bromoform (CHBr3) transfer to milk. Four multiparous Holstein cows (684 ± 56 kg BW, 35 ± 2 kg/d milk yield, and 146 ± 12 d in milk (DIM) were randomly assigned to a 4 × 4 Latin square design. Cows received 1 of 4 treatments consisting of dietary inclusion of Asparagopsis armata at 0, 0.15, 0.30, and 0.45% of diet DM, corresponding to CTRL, AA15, AA30, and AA45, respectively. The AA used had a concentration of 3 mg of bromoform/g of DM. Each 21-d period included 14 d of adaptation and 7 d of measurement, during which dry matter intake (DMI), milk yield and composition, CHBr3 transfer, methane emissions, and feeding behavior were measured. Enteric CH4 was measured using a Laser Methane Detector (Crowcon Laser Methane Mini, Tokyo Gas Engineering Co. Ltd., Tokyo, Japan). Cows were fed total mixed ration (TMR) ad libitum (5–10% refusals). Data were analyzed with the MIXED procedure of SAS. Feeding AA reduced CH4 production, yield, and intensity linearly by up to ~25%. Dry matter intake, milk yield, ECM, and BW were unaffected. Milk protein remained unchanged, but fat declined linearly with AA inclusion. Meal duration decreased linearly, and no CHBr3 was detected in milk. Asparagopsis armata shows strong potential as a feed additive to mitigate enteric CH4 without compromising production. Further studies should assess long-term effects on milk fat concentration and feeding behavior.
EEA Manfredi
Fil: Armand, Ezequiel. Universidad Nacional de Córdoba (UNC). Facultad de Ciencias Agropecuarias; Argentina
Fil: Salloum, María Soraya. Universidad Nacional de Córdoba (UNC). Facultad de Ciencias Agropecuarias; Argentina
Fil: Gimenez, Ruben Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi; Argentina
Fil: Gimenez, Ruben Alejandro. Universidad Nacional de Córdoba (UNC). Facultad de Ciencias Agropecuarias; Argentina
Fil: Di Lorenzo, Nicolas. University of Florida. North Florida Research and Education Center; Estados Unidos
Fil: De León, Marcelo. Universidad Nacional de Córdoba (UNC). Facultad de Ciencias Agropecuarias; Argentina
Fil: Zenobi, Marcos Gonzalo. Universidad Nacional de Córdoba (UNC). Facultad de Ciencias Agropecuarias; Argentina
Fuente
JDS Communications : 1-5 (Available online 23 April 2026)
Materia
Alimentación de los Animales
Asparagopsis armata
Metano Entérico
Producción Lechera
Vacas Lecheras
Animal Feeding
Enteric Methane
Milk Production
Dairy Cows
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/26640
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oai_identifier_str oai:localhost:20.500.12123/26640
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spelling Feeding increasing amounts of Asparagopsis armata decreases enteric methane yield without compromising production in lactating dairy cowsArmand, EzequielSalloum, María SorayaGimenez, Ruben AlejandroDi Lorenzo, NicolasDe León, MarceloZenobi, Marcos GonzaloAlimentación de los AnimalesAsparagopsis armataMetano EntéricoProducción LecheraVacas LecherasAnimal FeedingEnteric MethaneMilk ProductionDairy CowsEnteric methane (CH4) is the primary greenhouse gas emitted by ruminants. The red seaweed Asparagopsis armata (AA) effectively mitigates CH4, though optimal inclusion rates remain uncertain. The objective was to evaluate the dose–response effects of increasing AA inclusion levels on CH4 emissions, animal performance, feeding behavior, and bromoform (CHBr3) transfer to milk. Four multiparous Holstein cows (684 ± 56 kg BW, 35 ± 2 kg/d milk yield, and 146 ± 12 d in milk (DIM) were randomly assigned to a 4 × 4 Latin square design. Cows received 1 of 4 treatments consisting of dietary inclusion of Asparagopsis armata at 0, 0.15, 0.30, and 0.45% of diet DM, corresponding to CTRL, AA15, AA30, and AA45, respectively. The AA used had a concentration of 3 mg of bromoform/g of DM. Each 21-d period included 14 d of adaptation and 7 d of measurement, during which dry matter intake (DMI), milk yield and composition, CHBr3 transfer, methane emissions, and feeding behavior were measured. Enteric CH4 was measured using a Laser Methane Detector (Crowcon Laser Methane Mini, Tokyo Gas Engineering Co. Ltd., Tokyo, Japan). Cows were fed total mixed ration (TMR) ad libitum (5–10% refusals). Data were analyzed with the MIXED procedure of SAS. Feeding AA reduced CH4 production, yield, and intensity linearly by up to ~25%. Dry matter intake, milk yield, ECM, and BW were unaffected. Milk protein remained unchanged, but fat declined linearly with AA inclusion. Meal duration decreased linearly, and no CHBr3 was detected in milk. Asparagopsis armata shows strong potential as a feed additive to mitigate enteric CH4 without compromising production. Further studies should assess long-term effects on milk fat concentration and feeding behavior.EEA ManfrediFil: Armand, Ezequiel. Universidad Nacional de Córdoba (UNC). Facultad de Ciencias Agropecuarias; ArgentinaFil: Salloum, María Soraya. Universidad Nacional de Córdoba (UNC). Facultad de Ciencias Agropecuarias; ArgentinaFil: Gimenez, Ruben Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi; ArgentinaFil: Gimenez, Ruben Alejandro. Universidad Nacional de Córdoba (UNC). Facultad de Ciencias Agropecuarias; ArgentinaFil: Di Lorenzo, Nicolas. University of Florida. North Florida Research and Education Center; Estados UnidosFil: De León, Marcelo. Universidad Nacional de Córdoba (UNC). Facultad de Ciencias Agropecuarias; ArgentinaFil: Zenobi, Marcos Gonzalo. Universidad Nacional de Córdoba (UNC). Facultad de Ciencias Agropecuarias; ArgentinaElsevier2026-06-16T14:22:14Z2026-06-16T14:22:14Z2026-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/26640https://www.sciencedirect.com/science/article/pii/S2666910226000840?via%3Dihub2666-9102https://doi.org/10.3168/jdsc.2025-0895JDS Communications : 1-5 (Available online 23 April 2026)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-09-24T11:43:20Zoai:localhost:20.500.12123/26640instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-09-24 11:43:22.067INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Feeding increasing amounts of Asparagopsis armata decreases enteric methane yield without compromising production in lactating dairy cows
title Feeding increasing amounts of Asparagopsis armata decreases enteric methane yield without compromising production in lactating dairy cows
spellingShingle Feeding increasing amounts of Asparagopsis armata decreases enteric methane yield without compromising production in lactating dairy cows
Armand, Ezequiel
Alimentación de los Animales
Asparagopsis armata
Metano Entérico
Producción Lechera
Vacas Lecheras
Animal Feeding
Enteric Methane
Milk Production
Dairy Cows
title_short Feeding increasing amounts of Asparagopsis armata decreases enteric methane yield without compromising production in lactating dairy cows
title_full Feeding increasing amounts of Asparagopsis armata decreases enteric methane yield without compromising production in lactating dairy cows
title_fullStr Feeding increasing amounts of Asparagopsis armata decreases enteric methane yield without compromising production in lactating dairy cows
title_full_unstemmed Feeding increasing amounts of Asparagopsis armata decreases enteric methane yield without compromising production in lactating dairy cows
title_sort Feeding increasing amounts of Asparagopsis armata decreases enteric methane yield without compromising production in lactating dairy cows
dc.creator.none.fl_str_mv Armand, Ezequiel
Salloum, María Soraya
Gimenez, Ruben Alejandro
Di Lorenzo, Nicolas
De León, Marcelo
Zenobi, Marcos Gonzalo
author Armand, Ezequiel
author_facet Armand, Ezequiel
Salloum, María Soraya
Gimenez, Ruben Alejandro
Di Lorenzo, Nicolas
De León, Marcelo
Zenobi, Marcos Gonzalo
author_role author
author2 Salloum, María Soraya
Gimenez, Ruben Alejandro
Di Lorenzo, Nicolas
De León, Marcelo
Zenobi, Marcos Gonzalo
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Alimentación de los Animales
Asparagopsis armata
Metano Entérico
Producción Lechera
Vacas Lecheras
Animal Feeding
Enteric Methane
Milk Production
Dairy Cows
topic Alimentación de los Animales
Asparagopsis armata
Metano Entérico
Producción Lechera
Vacas Lecheras
Animal Feeding
Enteric Methane
Milk Production
Dairy Cows
dc.description.none.fl_txt_mv Enteric methane (CH4) is the primary greenhouse gas emitted by ruminants. The red seaweed Asparagopsis armata (AA) effectively mitigates CH4, though optimal inclusion rates remain uncertain. The objective was to evaluate the dose–response effects of increasing AA inclusion levels on CH4 emissions, animal performance, feeding behavior, and bromoform (CHBr3) transfer to milk. Four multiparous Holstein cows (684 ± 56 kg BW, 35 ± 2 kg/d milk yield, and 146 ± 12 d in milk (DIM) were randomly assigned to a 4 × 4 Latin square design. Cows received 1 of 4 treatments consisting of dietary inclusion of Asparagopsis armata at 0, 0.15, 0.30, and 0.45% of diet DM, corresponding to CTRL, AA15, AA30, and AA45, respectively. The AA used had a concentration of 3 mg of bromoform/g of DM. Each 21-d period included 14 d of adaptation and 7 d of measurement, during which dry matter intake (DMI), milk yield and composition, CHBr3 transfer, methane emissions, and feeding behavior were measured. Enteric CH4 was measured using a Laser Methane Detector (Crowcon Laser Methane Mini, Tokyo Gas Engineering Co. Ltd., Tokyo, Japan). Cows were fed total mixed ration (TMR) ad libitum (5–10% refusals). Data were analyzed with the MIXED procedure of SAS. Feeding AA reduced CH4 production, yield, and intensity linearly by up to ~25%. Dry matter intake, milk yield, ECM, and BW were unaffected. Milk protein remained unchanged, but fat declined linearly with AA inclusion. Meal duration decreased linearly, and no CHBr3 was detected in milk. Asparagopsis armata shows strong potential as a feed additive to mitigate enteric CH4 without compromising production. Further studies should assess long-term effects on milk fat concentration and feeding behavior.
EEA Manfredi
Fil: Armand, Ezequiel. Universidad Nacional de Córdoba (UNC). Facultad de Ciencias Agropecuarias; Argentina
Fil: Salloum, María Soraya. Universidad Nacional de Córdoba (UNC). Facultad de Ciencias Agropecuarias; Argentina
Fil: Gimenez, Ruben Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi; Argentina
Fil: Gimenez, Ruben Alejandro. Universidad Nacional de Córdoba (UNC). Facultad de Ciencias Agropecuarias; Argentina
Fil: Di Lorenzo, Nicolas. University of Florida. North Florida Research and Education Center; Estados Unidos
Fil: De León, Marcelo. Universidad Nacional de Córdoba (UNC). Facultad de Ciencias Agropecuarias; Argentina
Fil: Zenobi, Marcos Gonzalo. Universidad Nacional de Córdoba (UNC). Facultad de Ciencias Agropecuarias; Argentina
description Enteric methane (CH4) is the primary greenhouse gas emitted by ruminants. The red seaweed Asparagopsis armata (AA) effectively mitigates CH4, though optimal inclusion rates remain uncertain. The objective was to evaluate the dose–response effects of increasing AA inclusion levels on CH4 emissions, animal performance, feeding behavior, and bromoform (CHBr3) transfer to milk. Four multiparous Holstein cows (684 ± 56 kg BW, 35 ± 2 kg/d milk yield, and 146 ± 12 d in milk (DIM) were randomly assigned to a 4 × 4 Latin square design. Cows received 1 of 4 treatments consisting of dietary inclusion of Asparagopsis armata at 0, 0.15, 0.30, and 0.45% of diet DM, corresponding to CTRL, AA15, AA30, and AA45, respectively. The AA used had a concentration of 3 mg of bromoform/g of DM. Each 21-d period included 14 d of adaptation and 7 d of measurement, during which dry matter intake (DMI), milk yield and composition, CHBr3 transfer, methane emissions, and feeding behavior were measured. Enteric CH4 was measured using a Laser Methane Detector (Crowcon Laser Methane Mini, Tokyo Gas Engineering Co. Ltd., Tokyo, Japan). Cows were fed total mixed ration (TMR) ad libitum (5–10% refusals). Data were analyzed with the MIXED procedure of SAS. Feeding AA reduced CH4 production, yield, and intensity linearly by up to ~25%. Dry matter intake, milk yield, ECM, and BW were unaffected. Milk protein remained unchanged, but fat declined linearly with AA inclusion. Meal duration decreased linearly, and no CHBr3 was detected in milk. Asparagopsis armata shows strong potential as a feed additive to mitigate enteric CH4 without compromising production. Further studies should assess long-term effects on milk fat concentration and feeding behavior.
publishDate 2026
dc.date.none.fl_str_mv 2026-06-16T14:22:14Z
2026-06-16T14:22:14Z
2026-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/26640
https://www.sciencedirect.com/science/article/pii/S2666910226000840?via%3Dihub
2666-9102
https://doi.org/10.3168/jdsc.2025-0895
url http://hdl.handle.net/20.500.12123/26640
https://www.sciencedirect.com/science/article/pii/S2666910226000840?via%3Dihub
https://doi.org/10.3168/jdsc.2025-0895
identifier_str_mv 2666-9102
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv JDS Communications : 1-5 (Available online 23 April 2026)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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