Interference of RB51 and Delta-PGM vaccination with serological diagnosis of bovine brucellosis in adult cows

Autores
Foster, Camila Nayla; Suarez Archilla, Guillermo; Zbrun, Maria Virginia; Gareis, Natalia Carolina; Garrappa, Gabriela; Signorini Porchiett, Marcelo Lisandro; Novoa, María Belen
Año de publicación
2026
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bovine brucellosis remains a major zoonosis, its control relies on vaccination, serological surveillance and culling of infected animals. In most countries where brucellosis is endemic, vaccination of calves with Brucella abortus strain S19 is mandatory. Booster vaccination of adult cattle with the RB51 and Delta-PGM vaccines may be voluntarily implemented to increase herd immunity. This booster vaccination in adult cows may interfere with serological diagnosis and compromise control programs. This study evaluated the antibody responses induced by the RB51 and Delta-PGM vaccines in adult lactating dairy cows vaccinated with S19 during calfhood, and their interference with diagnostic assays. Three groups of ten Holstein cows from a brucellosis-free herd were established: RB51, Delta-PGM, and control. Blood and milk samples were collected weekly for 180 days post-vaccination. Antibody responses were assessed using buffered plate sera agglutination test (BPA), complement fixation test (CFT), fluorescence polarization assay (FPA), indirect and competitive ELISAs (iELISA, cELISA) in sera, and iELISA in milk. RB51 did not induce detectable antibody responses by any of the assays. Delta-PGM vaccination induced a transient humoral response, with the highest proportion of positive animals detected between 14 and 43 days post-vaccination. Antibodies remained detectable for up to two months with CFT and cELISA and up to four months with BPA, FPA, iELISA in sera and milk. These findings indicate that RB51 is compatible with serological surveillance–based control programs, whereas Delta-PGM may interfere with diagnostic interpretation for several months after vaccination. This information is relevant for brucellosis control programs in endemic areas.
EEA Rafaela
Fil: Foster, Camila Nayla. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Foster, Camila Nayla. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Suarez Archilla, Guillermo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Suarez Archilla, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Zbrun, Maria Virginia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Zbrun, Maria Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Gareis, Natalia Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Gareis, Natalia Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Garrappa, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Garrapa, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Signorini, Marcelo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Signorini, Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IdICaL); Argentina
Fil: Novoa, María Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Novoa, María Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fuente
Preventive Veterinary Medicine: Regional Studies and Reports 1 (3) : Article: 100052. (December 2026)
Materia
Ganado Bovino
Ganado de Leche
Enfermedades de los Animales
Brucelosis
Inmunodiagnóstico
Vacunación
Cattle
Dairy Cattle
Animal Diseases
Brucellosis
Immunodiagnosis
Vaccination
Diagnóstico Serológico
Serological Diagnosis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/27675
Acceder
id INTADig_3e657ed8e4bd0922a52bc6abfc9221a3
oai_identifier_str oai:localhost:20.500.12123/27675
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Interference of RB51 and Delta-PGM vaccination with serological diagnosis of bovine brucellosis in adult cowsFoster, Camila NaylaSuarez Archilla, GuillermoZbrun, Maria VirginiaGareis, Natalia CarolinaGarrappa, GabrielaSignorini Porchiett, Marcelo LisandroNovoa, María BelenGanado BovinoGanado de LecheEnfermedades de los AnimalesBrucelosisInmunodiagnósticoVacunaciónCattleDairy CattleAnimal DiseasesBrucellosisImmunodiagnosisVaccinationDiagnóstico SerológicoSerological DiagnosisBovine brucellosis remains a major zoonosis, its control relies on vaccination, serological surveillance and culling of infected animals. In most countries where brucellosis is endemic, vaccination of calves with Brucella abortus strain S19 is mandatory. Booster vaccination of adult cattle with the RB51 and Delta-PGM vaccines may be voluntarily implemented to increase herd immunity. This booster vaccination in adult cows may interfere with serological diagnosis and compromise control programs. This study evaluated the antibody responses induced by the RB51 and Delta-PGM vaccines in adult lactating dairy cows vaccinated with S19 during calfhood, and their interference with diagnostic assays. Three groups of ten Holstein cows from a brucellosis-free herd were established: RB51, Delta-PGM, and control. Blood and milk samples were collected weekly for 180 days post-vaccination. Antibody responses were assessed using buffered plate sera agglutination test (BPA), complement fixation test (CFT), fluorescence polarization assay (FPA), indirect and competitive ELISAs (iELISA, cELISA) in sera, and iELISA in milk. RB51 did not induce detectable antibody responses by any of the assays. Delta-PGM vaccination induced a transient humoral response, with the highest proportion of positive animals detected between 14 and 43 days post-vaccination. Antibodies remained detectable for up to two months with CFT and cELISA and up to four months with BPA, FPA, iELISA in sera and milk. These findings indicate that RB51 is compatible with serological surveillance–based control programs, whereas Delta-PGM may interfere with diagnostic interpretation for several months after vaccination. This information is relevant for brucellosis control programs in endemic areas.EEA RafaelaFil: Foster, Camila Nayla. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Foster, Camila Nayla. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Suarez Archilla, Guillermo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Suarez Archilla, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Zbrun, Maria Virginia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Zbrun, Maria Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Gareis, Natalia Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Gareis, Natalia Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Garrappa, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Garrapa, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Signorini, Marcelo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Signorini, Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IdICaL); ArgentinaFil: Novoa, María Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Novoa, María Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaElsevier2026-09-07T13:41:05Z2026-09-07T13:41:05Z2026-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/27675https://www.sciencedirect.com/science/article/pii/S30513081260005253051-3081https://doi.org/10.1016/j.pvmrsr.2026.100052Preventive Veterinary Medicine: Regional Studies and Reports 1 (3) : Article: 100052. (December 2026)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2023-PD-L06-I114, Desarrollo y aplicación de métodos diagnósticos y epidemiológicos para la producción pecuaria sustentable y agroalimentaria en humanos con foco en Una Saludinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-09-24T11:43:47Zoai:localhost:20.500.12123/27675instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-09-24 11:43:48.411INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Interference of RB51 and Delta-PGM vaccination with serological diagnosis of bovine brucellosis in adult cows
title Interference of RB51 and Delta-PGM vaccination with serological diagnosis of bovine brucellosis in adult cows
spellingShingle Interference of RB51 and Delta-PGM vaccination with serological diagnosis of bovine brucellosis in adult cows
Foster, Camila Nayla
Ganado Bovino
Ganado de Leche
Enfermedades de los Animales
Brucelosis
Inmunodiagnóstico
Vacunación
Cattle
Dairy Cattle
Animal Diseases
Brucellosis
Immunodiagnosis
Vaccination
Diagnóstico Serológico
Serological Diagnosis
title_short Interference of RB51 and Delta-PGM vaccination with serological diagnosis of bovine brucellosis in adult cows
title_full Interference of RB51 and Delta-PGM vaccination with serological diagnosis of bovine brucellosis in adult cows
title_fullStr Interference of RB51 and Delta-PGM vaccination with serological diagnosis of bovine brucellosis in adult cows
title_full_unstemmed Interference of RB51 and Delta-PGM vaccination with serological diagnosis of bovine brucellosis in adult cows
title_sort Interference of RB51 and Delta-PGM vaccination with serological diagnosis of bovine brucellosis in adult cows
dc.creator.none.fl_str_mv Foster, Camila Nayla
Suarez Archilla, Guillermo
Zbrun, Maria Virginia
Gareis, Natalia Carolina
Garrappa, Gabriela
Signorini Porchiett, Marcelo Lisandro
Novoa, María Belen
author Foster, Camila Nayla
author_facet Foster, Camila Nayla
Suarez Archilla, Guillermo
Zbrun, Maria Virginia
Gareis, Natalia Carolina
Garrappa, Gabriela
Signorini Porchiett, Marcelo Lisandro
Novoa, María Belen
author_role author
author2 Suarez Archilla, Guillermo
Zbrun, Maria Virginia
Gareis, Natalia Carolina
Garrappa, Gabriela
Signorini Porchiett, Marcelo Lisandro
Novoa, María Belen
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Ganado Bovino
Ganado de Leche
Enfermedades de los Animales
Brucelosis
Inmunodiagnóstico
Vacunación
Cattle
Dairy Cattle
Animal Diseases
Brucellosis
Immunodiagnosis
Vaccination
Diagnóstico Serológico
Serological Diagnosis
topic Ganado Bovino
Ganado de Leche
Enfermedades de los Animales
Brucelosis
Inmunodiagnóstico
Vacunación
Cattle
Dairy Cattle
Animal Diseases
Brucellosis
Immunodiagnosis
Vaccination
Diagnóstico Serológico
Serological Diagnosis
dc.description.none.fl_txt_mv Bovine brucellosis remains a major zoonosis, its control relies on vaccination, serological surveillance and culling of infected animals. In most countries where brucellosis is endemic, vaccination of calves with Brucella abortus strain S19 is mandatory. Booster vaccination of adult cattle with the RB51 and Delta-PGM vaccines may be voluntarily implemented to increase herd immunity. This booster vaccination in adult cows may interfere with serological diagnosis and compromise control programs. This study evaluated the antibody responses induced by the RB51 and Delta-PGM vaccines in adult lactating dairy cows vaccinated with S19 during calfhood, and their interference with diagnostic assays. Three groups of ten Holstein cows from a brucellosis-free herd were established: RB51, Delta-PGM, and control. Blood and milk samples were collected weekly for 180 days post-vaccination. Antibody responses were assessed using buffered plate sera agglutination test (BPA), complement fixation test (CFT), fluorescence polarization assay (FPA), indirect and competitive ELISAs (iELISA, cELISA) in sera, and iELISA in milk. RB51 did not induce detectable antibody responses by any of the assays. Delta-PGM vaccination induced a transient humoral response, with the highest proportion of positive animals detected between 14 and 43 days post-vaccination. Antibodies remained detectable for up to two months with CFT and cELISA and up to four months with BPA, FPA, iELISA in sera and milk. These findings indicate that RB51 is compatible with serological surveillance–based control programs, whereas Delta-PGM may interfere with diagnostic interpretation for several months after vaccination. This information is relevant for brucellosis control programs in endemic areas.
EEA Rafaela
Fil: Foster, Camila Nayla. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Foster, Camila Nayla. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Suarez Archilla, Guillermo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Suarez Archilla, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Zbrun, Maria Virginia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Zbrun, Maria Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Gareis, Natalia Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Gareis, Natalia Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Garrappa, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Garrapa, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Signorini, Marcelo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Signorini, Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IdICaL); Argentina
Fil: Novoa, María Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Novoa, María Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
description Bovine brucellosis remains a major zoonosis, its control relies on vaccination, serological surveillance and culling of infected animals. In most countries where brucellosis is endemic, vaccination of calves with Brucella abortus strain S19 is mandatory. Booster vaccination of adult cattle with the RB51 and Delta-PGM vaccines may be voluntarily implemented to increase herd immunity. This booster vaccination in adult cows may interfere with serological diagnosis and compromise control programs. This study evaluated the antibody responses induced by the RB51 and Delta-PGM vaccines in adult lactating dairy cows vaccinated with S19 during calfhood, and their interference with diagnostic assays. Three groups of ten Holstein cows from a brucellosis-free herd were established: RB51, Delta-PGM, and control. Blood and milk samples were collected weekly for 180 days post-vaccination. Antibody responses were assessed using buffered plate sera agglutination test (BPA), complement fixation test (CFT), fluorescence polarization assay (FPA), indirect and competitive ELISAs (iELISA, cELISA) in sera, and iELISA in milk. RB51 did not induce detectable antibody responses by any of the assays. Delta-PGM vaccination induced a transient humoral response, with the highest proportion of positive animals detected between 14 and 43 days post-vaccination. Antibodies remained detectable for up to two months with CFT and cELISA and up to four months with BPA, FPA, iELISA in sera and milk. These findings indicate that RB51 is compatible with serological surveillance–based control programs, whereas Delta-PGM may interfere with diagnostic interpretation for several months after vaccination. This information is relevant for brucellosis control programs in endemic areas.
publishDate 2026
dc.date.none.fl_str_mv 2026-09-07T13:41:05Z
2026-09-07T13:41:05Z
2026-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/27675
https://www.sciencedirect.com/science/article/pii/S3051308126000525
3051-3081
https://doi.org/10.1016/j.pvmrsr.2026.100052
url http://hdl.handle.net/20.500.12123/27675
https://www.sciencedirect.com/science/article/pii/S3051308126000525
https://doi.org/10.1016/j.pvmrsr.2026.100052
identifier_str_mv 3051-3081
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2023-PD-L06-I114, Desarrollo y aplicación de métodos diagnósticos y epidemiológicos para la producción pecuaria sustentable y agroalimentaria en humanos con foco en Una Salud
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Preventive Veterinary Medicine: Regional Studies and Reports 1 (3) : Article: 100052. (December 2026)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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