Genomic Characterization of Aerobically Culturable Gut-Associated Bacteria and Yeasts Isolated from Pooled Larval Midguts of the Lesser Mealworm Alphitobius diaperinus (Coleoptera:...

Autores: Antonuccio, Gisele Ivonne; Lopez, Pablo Julian; Berretta, Marcelo Facundo; Sauka, Diego Hernan
Año de publicación: 2026
Idioma: inglés
Tipo de recurso: artículo
Estado: versión publicada
Descripción: Microbial communities associated with insect larvae play key roles in host nutrition, protection against pathogens, immune modulation, and represent a source of biotechnologically relevant traits. In Alphitobius diaperinus, a major poultry pest, the gut microbiota has been mainly studied at the community level, providing limited insight into the potential roles of individual microorganisms. Here, we characterized the aerobically culturable fraction of the larval gut microbiota derived from a single pooled homogenate of fifteen larvae under stable laboratory rearing conditions—an approach that uniquely enables strain-level genomic resolution, functional experimentation, and biotechnological exploitation. Ten bacterial strains and two yeasts were initially isolated; eight bacterial isolates were identical, revealing the most frequently recovered cultivable bacterial morphotype. We provide high-quality draft genomes for five members of the A. diaperinus cultivable gut microbiota—Enterobacter hormaechei subsp. xiangfangensis strain INTA AN1-1, Staphylococcus hominis subsp. novobiosepticus strain INTA AC1-4, Staphylococcus succinus subsp. succinus strain INTA AC1-8, Hyphopichia burtonii strain INTA AB1-1, and Debaryomyces fabryi strain INTA AB1-4—establishing a genome-resolved reference framework validated through a polyphasic approach. Genome-scale annotation enabled us to predict the metabolic potential and hypothesize the functional capabilities among cultivable members, providing a foundation for future studies on this economically relevant pest.
Instituto de Microbiología y Zoología Agrícola (IMYZA)
Fil: Antonuccio, Gisele. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina
Fil: Antonuccio, Gisele. Provincia de Buenos Aires. Comisión de Investigaciones Científicas (CIC); Argentina
Fil: López, Pablo Julián. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina
Fil: Berretta, Marcelo Facundo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina
Fil: Berretta, Marcelo Facundo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sauka, Diego Herman. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina
Fil: Sauka, Diego Herman. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente: Insects 17 (8) : 800 (August 2026)
Materia: Microbiology
Ecology
Genomics
Bacteria
Insects
Pests
Microbiología
Ecología
Genómica
Insectos
Plagas
Alphitobius diaperinus
Nivel de accesibilidad: acceso abierto
Condiciones de uso: http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
Institución: Instituto Nacional de Tecnología Agropecuaria
OAI Identificador: oai:localhost:20.500.12123/27613

Acceder

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spelling	Genomic Characterization of Aerobically Culturable Gut-Associated Bacteria and Yeasts Isolated from Pooled Larval Midguts of the Lesser Mealworm Alphitobius diaperinus (Coleoptera: Tenebrionidae)Antonuccio, Gisele IvonneLopez, Pablo JulianBerretta, Marcelo FacundoSauka, Diego HernanMicrobiologyEcologyGenomicsBacteriaInsectsPestsMicrobiologíaEcologíaGenómicaInsectosPlagasAlphitobius diaperinusMicrobial communities associated with insect larvae play key roles in host nutrition, protection against pathogens, immune modulation, and represent a source of biotechnologically relevant traits. In Alphitobius diaperinus, a major poultry pest, the gut microbiota has been mainly studied at the community level, providing limited insight into the potential roles of individual microorganisms. Here, we characterized the aerobically culturable fraction of the larval gut microbiota derived from a single pooled homogenate of fifteen larvae under stable laboratory rearing conditions—an approach that uniquely enables strain-level genomic resolution, functional experimentation, and biotechnological exploitation. Ten bacterial strains and two yeasts were initially isolated; eight bacterial isolates were identical, revealing the most frequently recovered cultivable bacterial morphotype. We provide high-quality draft genomes for five members of the A. diaperinus cultivable gut microbiota—Enterobacter hormaechei subsp. xiangfangensis strain INTA AN1-1, Staphylococcus hominis subsp. novobiosepticus strain INTA AC1-4, Staphylococcus succinus subsp. succinus strain INTA AC1-8, Hyphopichia burtonii strain INTA AB1-1, and Debaryomyces fabryi strain INTA AB1-4—establishing a genome-resolved reference framework validated through a polyphasic approach. Genome-scale annotation enabled us to predict the metabolic potential and hypothesize the functional capabilities among cultivable members, providing a foundation for future studies on this economically relevant pest.Instituto de Microbiología y Zoología Agrícola (IMYZA)Fil: Antonuccio, Gisele. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); ArgentinaFil: Antonuccio, Gisele. Provincia de Buenos Aires. Comisión de Investigaciones Científicas (CIC); ArgentinaFil: López, Pablo Julián. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); ArgentinaFil: Berretta, Marcelo Facundo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); ArgentinaFil: Berretta, Marcelo Facundo. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sauka, Diego Herman. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); ArgentinaFil: Sauka, Diego Herman. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaMDPI2026-09-02T12:18:54Z2026-09-02T12:18:54Z2026-08-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/27613https://www.mdpi.com/2075-4450/17/8/8002075-4450https://doi.org/10.3390/insects17080800Insects 17 (8) : 800 (August 2026)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2023-PD-L06-I116, Implementación de tecnologías y nuevas estrategias preventivas y terapéuticas para el desarrollo sustentable y eficiente de la producción animal en el marco de Una Saludinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-09-24T11:43:45Zoai:localhost:20.500.12123/27613instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-09-24 11:43:46.679INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv	Genomic Characterization of Aerobically Culturable Gut-Associated Bacteria and Yeasts Isolated from Pooled Larval Midguts of the Lesser Mealworm Alphitobius diaperinus (Coleoptera: Tenebrionidae)
title	Genomic Characterization of Aerobically Culturable Gut-Associated Bacteria and Yeasts Isolated from Pooled Larval Midguts of the Lesser Mealworm Alphitobius diaperinus (Coleoptera: Tenebrionidae)
spellingShingle	Genomic Characterization of Aerobically Culturable Gut-Associated Bacteria and Yeasts Isolated from Pooled Larval Midguts of the Lesser Mealworm Alphitobius diaperinus (Coleoptera: Tenebrionidae) Antonuccio, Gisele Ivonne Microbiology Ecology Genomics Bacteria Insects Pests Microbiología Ecología Genómica Insectos Plagas Alphitobius diaperinus
title_short	Genomic Characterization of Aerobically Culturable Gut-Associated Bacteria and Yeasts Isolated from Pooled Larval Midguts of the Lesser Mealworm Alphitobius diaperinus (Coleoptera: Tenebrionidae)
title_full	Genomic Characterization of Aerobically Culturable Gut-Associated Bacteria and Yeasts Isolated from Pooled Larval Midguts of the Lesser Mealworm Alphitobius diaperinus (Coleoptera: Tenebrionidae)
title_fullStr	Genomic Characterization of Aerobically Culturable Gut-Associated Bacteria and Yeasts Isolated from Pooled Larval Midguts of the Lesser Mealworm Alphitobius diaperinus (Coleoptera: Tenebrionidae)
title_full_unstemmed	Genomic Characterization of Aerobically Culturable Gut-Associated Bacteria and Yeasts Isolated from Pooled Larval Midguts of the Lesser Mealworm Alphitobius diaperinus (Coleoptera: Tenebrionidae)
title_sort	Genomic Characterization of Aerobically Culturable Gut-Associated Bacteria and Yeasts Isolated from Pooled Larval Midguts of the Lesser Mealworm Alphitobius diaperinus (Coleoptera: Tenebrionidae)
dc.creator.none.fl_str_mv	Antonuccio, Gisele Ivonne Lopez, Pablo Julian Berretta, Marcelo Facundo Sauka, Diego Hernan
author	Antonuccio, Gisele Ivonne
author_facet	Antonuccio, Gisele Ivonne Lopez, Pablo Julian Berretta, Marcelo Facundo Sauka, Diego Hernan
author_role	author
author2	Lopez, Pablo Julian Berretta, Marcelo Facundo Sauka, Diego Hernan
author2_role	author author author
dc.subject.none.fl_str_mv	Microbiology Ecology Genomics Bacteria Insects Pests Microbiología Ecología Genómica Insectos Plagas Alphitobius diaperinus
topic	Microbiology Ecology Genomics Bacteria Insects Pests Microbiología Ecología Genómica Insectos Plagas Alphitobius diaperinus
dc.description.none.fl_txt_mv	Microbial communities associated with insect larvae play key roles in host nutrition, protection against pathogens, immune modulation, and represent a source of biotechnologically relevant traits. In Alphitobius diaperinus, a major poultry pest, the gut microbiota has been mainly studied at the community level, providing limited insight into the potential roles of individual microorganisms. Here, we characterized the aerobically culturable fraction of the larval gut microbiota derived from a single pooled homogenate of fifteen larvae under stable laboratory rearing conditions—an approach that uniquely enables strain-level genomic resolution, functional experimentation, and biotechnological exploitation. Ten bacterial strains and two yeasts were initially isolated; eight bacterial isolates were identical, revealing the most frequently recovered cultivable bacterial morphotype. We provide high-quality draft genomes for five members of the A. diaperinus cultivable gut microbiota—Enterobacter hormaechei subsp. xiangfangensis strain INTA AN1-1, Staphylococcus hominis subsp. novobiosepticus strain INTA AC1-4, Staphylococcus succinus subsp. succinus strain INTA AC1-8, Hyphopichia burtonii strain INTA AB1-1, and Debaryomyces fabryi strain INTA AB1-4—establishing a genome-resolved reference framework validated through a polyphasic approach. Genome-scale annotation enabled us to predict the metabolic potential and hypothesize the functional capabilities among cultivable members, providing a foundation for future studies on this economically relevant pest. Instituto de Microbiología y Zoología Agrícola (IMYZA) Fil: Antonuccio, Gisele. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina Fil: Antonuccio, Gisele. Provincia de Buenos Aires. Comisión de Investigaciones Científicas (CIC); Argentina Fil: López, Pablo Julián. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina Fil: Berretta, Marcelo Facundo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina Fil: Berretta, Marcelo Facundo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Sauka, Diego Herman. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina Fil: Sauka, Diego Herman. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description	Microbial communities associated with insect larvae play key roles in host nutrition, protection against pathogens, immune modulation, and represent a source of biotechnologically relevant traits. In Alphitobius diaperinus, a major poultry pest, the gut microbiota has been mainly studied at the community level, providing limited insight into the potential roles of individual microorganisms. Here, we characterized the aerobically culturable fraction of the larval gut microbiota derived from a single pooled homogenate of fifteen larvae under stable laboratory rearing conditions—an approach that uniquely enables strain-level genomic resolution, functional experimentation, and biotechnological exploitation. Ten bacterial strains and two yeasts were initially isolated; eight bacterial isolates were identical, revealing the most frequently recovered cultivable bacterial morphotype. We provide high-quality draft genomes for five members of the A. diaperinus cultivable gut microbiota—Enterobacter hormaechei subsp. xiangfangensis strain INTA AN1-1, Staphylococcus hominis subsp. novobiosepticus strain INTA AC1-4, Staphylococcus succinus subsp. succinus strain INTA AC1-8, Hyphopichia burtonii strain INTA AB1-1, and Debaryomyces fabryi strain INTA AB1-4—establishing a genome-resolved reference framework validated through a polyphasic approach. Genome-scale annotation enabled us to predict the metabolic potential and hypothesize the functional capabilities among cultivable members, providing a foundation for future studies on this economically relevant pest.
publishDate	2026
dc.date.none.fl_str_mv	2026-09-02T12:18:54Z 2026-09-02T12:18:54Z 2026-08-02
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo
format	article
status_str	publishedVersion
dc.identifier.none.fl_str_mv	http://hdl.handle.net/20.500.12123/27613 https://www.mdpi.com/2075-4450/17/8/800 2075-4450 https://doi.org/10.3390/insects17080800
url	http://hdl.handle.net/20.500.12123/27613 https://www.mdpi.com/2075-4450/17/8/800 https://doi.org/10.3390/insects17080800
identifier_str_mv	2075-4450
dc.language.none.fl_str_mv	eng
language	eng
dc.relation.none.fl_str_mv	info:eu-repograntAgreement/INTA/2023-PD-L06-I116, Implementación de tecnologías y nuevas estrategias preventivas y terapéuticas para el desarrollo sustentable y eficiente de la producción animal en el marco de Una Salud
dc.rights.none.fl_str_mv	info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv	openAccess
rights_invalid_str_mv	http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv	application/pdf
dc.publisher.none.fl_str_mv	MDPI
publisher.none.fl_str_mv	MDPI
dc.source.none.fl_str_mv	Insects 17 (8) : 800 (August 2026) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str	INTA Digital (INTA)
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instname_str	Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv	INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv	tripaldi.nicolas@inta.gob.ar
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Genomic Characterization of Aerobically Culturable Gut-Associated Bacteria and Yeasts Isolated from Pooled Larval Midguts of the Lesser Mealworm Alphitobius diaperinus (Coleoptera:...

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