Optimization of the extraction conditions of bioactive compounds from Ocimum basilicum leaves using ultrasound-assisted extraction via sonotrode.

Autores
Aloisio, Carolina; Razola-Díaz, María del Carmen; Aznar-Ramos, María José; Longhi, Marcela; Andreatta, Alfonsina E.; Verardo, Vito
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Sweet basil (Ocimum basilicum) leaves are rich in bioactive compounds that present therapeutic benefits for human health. Ultrasonic-assisted extraction (UAE) is frequently used to obtain phenolic compounds from plants/herbal sources. However, few works have developed multi-variable studies to find the optimal conditions to extract the maximum amount of compounds, especially when applied to UAE via a sonotrode. The purpose of this work was to perform a multi-variable study by employing a Box–Behnken design to collect the highest active compound content from Ocimum basilicum leaves. The efficacy of the design was endorsed by ANOVA. The studied parameters for UAE via a sonotrode were the ethanol/water ratio, amplitude, and time. The analyzed responses were the rosmarinic acid, the sum of phenolic acids, and the sum of phenolic compounds content. The optimal conditions were found to be 50% ethanol/water, 50% amplitude, and 5 min. Twenty bioactive compounds were identified by HPLC-ESI-TOF-MS when the extract was collected by applying the optimal conditions. Ocimum basilicum may be appreciated as a valuable source of important bioactive substances for pharmaceutical use.
The authors also wish to acknowledge the financial support and assistance of Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), the Universidad Tecnológica Nacional (UTN), Universidad Nacional de Cordoba (UNC), and the University of Granada (UGR).
Fil: Aloisio, Carolina. Universidad Tecnológica Nacional. Facultad Regional San Francisco. Grupo UTN Ingeniería de Procesos Sustentables - CONICET. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica (UNITEFA); Argentina.
Fil: Razola-Diaz, María del Carmen. Department of Nutrition and Food Science, Campus of Cartuja, University of Granada, 18071 - Institute of Nutrition and Food Technology ‘José Matáix’, Biomedical Research Center, University of Granada, Avda del Conocimiento s/n., 18100. Granada; Spain
Fil: Aznar-Ramos, María José. Department of Nutrition and Food Science, Campus of Cartuja, University of Granada, 18071 - Institute of Nutrition and Food Technology ‘José Matáix’, Biomedical Research Center, University of Granada, Avda del Conocimiento s/n., 18100. Granada; Spain
Fil: Longhi, Marcela. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Ciencias Farmacéuticas. Córdoba; Argentina.
Fil: Andreatta, Alfonsina E. Universidad Tecnológica Nacional, Facultad Regional San Francisco, Grupo UTN Ingeniería de Procesos Sustentables. San Francisco; Argentina.
Fil: Verardo, Vito. Department of Nutrition and Food Science, Campus of Cartuja, University of Granada, 18071 - Institute of Nutrition and Food Technology ‘José Matáix’, Biomedical Research Center, University of Granada, Avda del Conocimiento s/n. 18100. Granada; Spain
Peer Reviewed
Fuente
Molecules 28(13), 5286(2023)
Materia
Sweet basil (Ocimum basilicum)
Ultrasonic-assisted extraction via a sonotrode
Box–Behnken design
HPLC-ESI-TOF-MS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
2024-03-06T23:26:03Z
Repositorio
Repositorio Institucional Abierto (UTN)
Institución
Universidad Tecnológica Nacional
OAI Identificador
oai:ria.utn.edu.ar:20.500.12272/9665
Acceder
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oai_identifier_str oai:ria.utn.edu.ar:20.500.12272/9665
network_acronym_str RIAUTN
repository_id_str a
network_name_str Repositorio Institucional Abierto (UTN)
spelling Optimization of the extraction conditions of bioactive compounds from Ocimum basilicum leaves using ultrasound-assisted extraction via sonotrode.Aloisio, CarolinaRazola-Díaz, María del CarmenAznar-Ramos, María JoséLonghi, MarcelaAndreatta, Alfonsina E.Verardo, VitoSweet basil (Ocimum basilicum)Ultrasonic-assisted extraction via a sonotrodeBox–Behnken designHPLC-ESI-TOF-MSSweet basil (Ocimum basilicum) leaves are rich in bioactive compounds that present therapeutic benefits for human health. Ultrasonic-assisted extraction (UAE) is frequently used to obtain phenolic compounds from plants/herbal sources. However, few works have developed multi-variable studies to find the optimal conditions to extract the maximum amount of compounds, especially when applied to UAE via a sonotrode. The purpose of this work was to perform a multi-variable study by employing a Box–Behnken design to collect the highest active compound content from Ocimum basilicum leaves. The efficacy of the design was endorsed by ANOVA. The studied parameters for UAE via a sonotrode were the ethanol/water ratio, amplitude, and time. The analyzed responses were the rosmarinic acid, the sum of phenolic acids, and the sum of phenolic compounds content. The optimal conditions were found to be 50% ethanol/water, 50% amplitude, and 5 min. Twenty bioactive compounds were identified by HPLC-ESI-TOF-MS when the extract was collected by applying the optimal conditions. Ocimum basilicum may be appreciated as a valuable source of important bioactive substances for pharmaceutical use.The authors also wish to acknowledge the financial support and assistance of Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), the Universidad Tecnológica Nacional (UTN), Universidad Nacional de Cordoba (UNC), and the University of Granada (UGR).Fil: Aloisio, Carolina. Universidad Tecnológica Nacional. Facultad Regional San Francisco. Grupo UTN Ingeniería de Procesos Sustentables - CONICET. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica (UNITEFA); Argentina.Fil: Razola-Diaz, María del Carmen. Department of Nutrition and Food Science, Campus of Cartuja, University of Granada, 18071 - Institute of Nutrition and Food Technology ‘José Matáix’, Biomedical Research Center, University of Granada, Avda del Conocimiento s/n., 18100. Granada; SpainFil: Aznar-Ramos, María José. Department of Nutrition and Food Science, Campus of Cartuja, University of Granada, 18071 - Institute of Nutrition and Food Technology ‘José Matáix’, Biomedical Research Center, University of Granada, Avda del Conocimiento s/n., 18100. Granada; SpainFil: Longhi, Marcela. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Ciencias Farmacéuticas. Córdoba; Argentina.Fil: Andreatta, Alfonsina E. Universidad Tecnológica Nacional, Facultad Regional San Francisco, Grupo UTN Ingeniería de Procesos Sustentables. San Francisco; Argentina.Fil: Verardo, Vito. Department of Nutrition and Food Science, Campus of Cartuja, University of Granada, 18071 - Institute of Nutrition and Food Technology ‘José Matáix’, Biomedical Research Center, University of Granada, Avda del Conocimiento s/n. 18100. Granada; SpainPeer Reviewed2024-03-06T23:26:03Z2024-03-06T23:26:03Z2023-07-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articulopdfapplication/pdfMolecules 2023, 28(13), 5286.1420-3049https://www.mdpi.com/1420-3049/28/13/5286http://hdl.handle.net/20.500.12272/9665https://doi.org/10.3390/molecules28135286Molecules 28(13), 5286(2023)reponame:Repositorio Institucional Abierto (UTN)instname:Universidad Tecnológica NacionalengengInternacionalinfo:eu-repo/semantics/openAccess2024-03-06T23:26:03Zhttp://creativecommons.org/licenses/by-nc-nd/4.0/Attribution-NonCommercial-NoDerivatives 4.0 Internacional.2026-09-24T12:45:21Zoai:ria.utn.edu.ar:20.500.12272/9665instacron:UTNInstitucionalhttp://ria.utn.edu.ar/Universidad públicaNo correspondehttp://ria.utn.edu.ar/oaigestionria@rec.utn.edu.ar; fsuarez@rec.utn.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2026-09-24 12:45:23.071Repositorio Institucional Abierto (UTN) - Universidad Tecnológica Nacionalfalse
dc.title.none.fl_str_mv Optimization of the extraction conditions of bioactive compounds from Ocimum basilicum leaves using ultrasound-assisted extraction via sonotrode.
title Optimization of the extraction conditions of bioactive compounds from Ocimum basilicum leaves using ultrasound-assisted extraction via sonotrode.
spellingShingle Optimization of the extraction conditions of bioactive compounds from Ocimum basilicum leaves using ultrasound-assisted extraction via sonotrode.
Aloisio, Carolina
Sweet basil (Ocimum basilicum)
Ultrasonic-assisted extraction via a sonotrode
Box–Behnken design
HPLC-ESI-TOF-MS
title_short Optimization of the extraction conditions of bioactive compounds from Ocimum basilicum leaves using ultrasound-assisted extraction via sonotrode.
title_full Optimization of the extraction conditions of bioactive compounds from Ocimum basilicum leaves using ultrasound-assisted extraction via sonotrode.
title_fullStr Optimization of the extraction conditions of bioactive compounds from Ocimum basilicum leaves using ultrasound-assisted extraction via sonotrode.
title_full_unstemmed Optimization of the extraction conditions of bioactive compounds from Ocimum basilicum leaves using ultrasound-assisted extraction via sonotrode.
title_sort Optimization of the extraction conditions of bioactive compounds from Ocimum basilicum leaves using ultrasound-assisted extraction via sonotrode.
dc.creator.none.fl_str_mv Aloisio, Carolina
Razola-Díaz, María del Carmen
Aznar-Ramos, María José
Longhi, Marcela
Andreatta, Alfonsina E.
Verardo, Vito
author Aloisio, Carolina
author_facet Aloisio, Carolina
Razola-Díaz, María del Carmen
Aznar-Ramos, María José
Longhi, Marcela
Andreatta, Alfonsina E.
Verardo, Vito
author_role author
author2 Razola-Díaz, María del Carmen
Aznar-Ramos, María José
Longhi, Marcela
Andreatta, Alfonsina E.
Verardo, Vito
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Sweet basil (Ocimum basilicum)
Ultrasonic-assisted extraction via a sonotrode
Box–Behnken design
HPLC-ESI-TOF-MS
topic Sweet basil (Ocimum basilicum)
Ultrasonic-assisted extraction via a sonotrode
Box–Behnken design
HPLC-ESI-TOF-MS
dc.description.none.fl_txt_mv Sweet basil (Ocimum basilicum) leaves are rich in bioactive compounds that present therapeutic benefits for human health. Ultrasonic-assisted extraction (UAE) is frequently used to obtain phenolic compounds from plants/herbal sources. However, few works have developed multi-variable studies to find the optimal conditions to extract the maximum amount of compounds, especially when applied to UAE via a sonotrode. The purpose of this work was to perform a multi-variable study by employing a Box–Behnken design to collect the highest active compound content from Ocimum basilicum leaves. The efficacy of the design was endorsed by ANOVA. The studied parameters for UAE via a sonotrode were the ethanol/water ratio, amplitude, and time. The analyzed responses were the rosmarinic acid, the sum of phenolic acids, and the sum of phenolic compounds content. The optimal conditions were found to be 50% ethanol/water, 50% amplitude, and 5 min. Twenty bioactive compounds were identified by HPLC-ESI-TOF-MS when the extract was collected by applying the optimal conditions. Ocimum basilicum may be appreciated as a valuable source of important bioactive substances for pharmaceutical use.
The authors also wish to acknowledge the financial support and assistance of Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), the Universidad Tecnológica Nacional (UTN), Universidad Nacional de Cordoba (UNC), and the University of Granada (UGR).
Fil: Aloisio, Carolina. Universidad Tecnológica Nacional. Facultad Regional San Francisco. Grupo UTN Ingeniería de Procesos Sustentables - CONICET. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica (UNITEFA); Argentina.
Fil: Razola-Diaz, María del Carmen. Department of Nutrition and Food Science, Campus of Cartuja, University of Granada, 18071 - Institute of Nutrition and Food Technology ‘José Matáix’, Biomedical Research Center, University of Granada, Avda del Conocimiento s/n., 18100. Granada; Spain
Fil: Aznar-Ramos, María José. Department of Nutrition and Food Science, Campus of Cartuja, University of Granada, 18071 - Institute of Nutrition and Food Technology ‘José Matáix’, Biomedical Research Center, University of Granada, Avda del Conocimiento s/n., 18100. Granada; Spain
Fil: Longhi, Marcela. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Ciencias Farmacéuticas. Córdoba; Argentina.
Fil: Andreatta, Alfonsina E. Universidad Tecnológica Nacional, Facultad Regional San Francisco, Grupo UTN Ingeniería de Procesos Sustentables. San Francisco; Argentina.
Fil: Verardo, Vito. Department of Nutrition and Food Science, Campus of Cartuja, University of Granada, 18071 - Institute of Nutrition and Food Technology ‘José Matáix’, Biomedical Research Center, University of Granada, Avda del Conocimiento s/n. 18100. Granada; Spain
Peer Reviewed
description Sweet basil (Ocimum basilicum) leaves are rich in bioactive compounds that present therapeutic benefits for human health. Ultrasonic-assisted extraction (UAE) is frequently used to obtain phenolic compounds from plants/herbal sources. However, few works have developed multi-variable studies to find the optimal conditions to extract the maximum amount of compounds, especially when applied to UAE via a sonotrode. The purpose of this work was to perform a multi-variable study by employing a Box–Behnken design to collect the highest active compound content from Ocimum basilicum leaves. The efficacy of the design was endorsed by ANOVA. The studied parameters for UAE via a sonotrode were the ethanol/water ratio, amplitude, and time. The analyzed responses were the rosmarinic acid, the sum of phenolic acids, and the sum of phenolic compounds content. The optimal conditions were found to be 50% ethanol/water, 50% amplitude, and 5 min. Twenty bioactive compounds were identified by HPLC-ESI-TOF-MS when the extract was collected by applying the optimal conditions. Ocimum basilicum may be appreciated as a valuable source of important bioactive substances for pharmaceutical use.
publishDate 2023
dc.date.none.fl_str_mv 2023-07-07
2024-03-06T23:26:03Z
2024-03-06T23:26:03Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv Molecules 2023, 28(13), 5286.
1420-3049
https://www.mdpi.com/1420-3049/28/13/5286
http://hdl.handle.net/20.500.12272/9665
https://doi.org/10.3390/molecules28135286
identifier_str_mv Molecules 2023, 28(13), 5286.
1420-3049
url https://www.mdpi.com/1420-3049/28/13/5286
http://hdl.handle.net/20.500.12272/9665
https://doi.org/10.3390/molecules28135286
dc.language.none.fl_str_mv eng
eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
2024-03-06T23:26:03Z
http://creativecommons.org/licenses/by-nc-nd/4.0/
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
.
eu_rights_str_mv openAccess
rights_invalid_str_mv 2024-03-06T23:26:03Z
http://creativecommons.org/licenses/by-nc-nd/4.0/
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
.
dc.format.none.fl_str_mv pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.source.none.fl_str_mv Molecules 28(13), 5286(2023)
reponame:Repositorio Institucional Abierto (UTN)
instname:Universidad Tecnológica Nacional
reponame_str Repositorio Institucional Abierto (UTN)
collection Repositorio Institucional Abierto (UTN)
instname_str Universidad Tecnológica Nacional
repository.name.fl_str_mv Repositorio Institucional Abierto (UTN) - Universidad Tecnológica Nacional
repository.mail.fl_str_mv gestionria@rec.utn.edu.ar; fsuarez@rec.utn.edu.ar
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