Enhancement of extraction parameters for bioactive substances from ocimum basilicum leaves through sonotrode-assisted ultrasonic extraction

Autores
Aloisio, Carolina; Razola-Díaz, María del Carmen; Aznar-Ramos, María José; Longhi, Marcela; Andreatta, Alfonsina E.; Verardo, Vito
Año de publicación
2023
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión aceptada
Descripción
Sweet basil (Ocimum basilicum) leaves are rich in bioactive compounds that present therapeutical benefits for human health. Ultrasonic-assisted extraction (UAE) is frequently used to obtain phenolic compounds in plants. However, few works have developed multivariable studies to find the optimal conditions to extract the maximum amount of compounds, especially applied to UAE via sonotrode. The purpose of this work was to perform a multi-variable study employing a Box–Behnken design to collect the highest active compound content from Ocimum basilicum leaves. The efficacy of the design was endorsed by ANOVA. The studied parameters for UAE via sonotrode were ethanol/water ratio, amplitude and time. The analyzed responses were the rosmarinic acid, sum of phenolic acids and sum of phenolic compounds content. Optimal conditions were found to be 50% ethanol/water, 50% amplitude and 5 min. 27 bioactive compounds were identified by HPLC-ESI-TOF-MS, when the extract was collected by applying the optimal conditions. Ocimum basilicum may be appreciated as a valuable source of important bioactive substances for pharmaceutical use.
CONICET, UNC, UTN, Universidad de Granada.
Fil: Aloisio, Carolina. UTN Fac. Reg. San Francisco. Ingeniería de Procesos Sustentables - Universidad Nacional de Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica (UNITEFA) - CONICET; Argentina.
Fil: Razola-Díaz, María del Carmen. University of Granada. Department of Nutrition and Food Science; Spain.
Fil: Aznar-Ramos, María José. University of Granada. Department of Nutrition and Food Science; Spain.
Fil: Longhi, Marcela. Universidad Nacional de Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica (UNITEFA) - CONICET; Argentina.
Fil: Andreatta, Alfonsina E. UTN Fac. Reg. San Francisco, Ingeniería de Procesos Sustentables - CONICET; Argentina.
Fil: Verardo, Vito. University of Granada. Department of Nutrition and Food Science; Spain.
Materia
Ocimum basilicum
Ultrasonic Extraction
Optimization
Nivel de accesibilidad
acceso abierto
Condiciones de uso
2024-03-26T19:02:54Z
Repositorio
Repositorio Institucional Abierto (UTN)
Institución
Universidad Tecnológica Nacional
OAI Identificador
oai:ria.utn.edu.ar:20.500.12272/10119
Acceder
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spelling Enhancement of extraction parameters for bioactive substances from ocimum basilicum leaves through sonotrode-assisted ultrasonic extractionAloisio, CarolinaRazola-Díaz, María del CarmenAznar-Ramos, María JoséLonghi, MarcelaAndreatta, Alfonsina E.Verardo, VitoOcimum basilicumUltrasonic ExtractionOptimizationSweet basil (Ocimum basilicum) leaves are rich in bioactive compounds that present therapeutical benefits for human health. Ultrasonic-assisted extraction (UAE) is frequently used to obtain phenolic compounds in plants. However, few works have developed multivariable studies to find the optimal conditions to extract the maximum amount of compounds, especially applied to UAE via sonotrode. The purpose of this work was to perform a multi-variable study employing a Box–Behnken design to collect the highest active compound content from Ocimum basilicum leaves. The efficacy of the design was endorsed by ANOVA. The studied parameters for UAE via sonotrode were ethanol/water ratio, amplitude and time. The analyzed responses were the rosmarinic acid, sum of phenolic acids and sum of phenolic compounds content. Optimal conditions were found to be 50% ethanol/water, 50% amplitude and 5 min. 27 bioactive compounds were identified by HPLC-ESI-TOF-MS, when the extract was collected by applying the optimal conditions. Ocimum basilicum may be appreciated as a valuable source of important bioactive substances for pharmaceutical use.CONICET, UNC, UTN, Universidad de Granada.Fil: Aloisio, Carolina. UTN Fac. Reg. San Francisco. Ingeniería de Procesos Sustentables - Universidad Nacional de Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica (UNITEFA) - CONICET; Argentina.Fil: Razola-Díaz, María del Carmen. University of Granada. Department of Nutrition and Food Science; Spain.Fil: Aznar-Ramos, María José. University of Granada. Department of Nutrition and Food Science; Spain.Fil: Longhi, Marcela. Universidad Nacional de Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica (UNITEFA) - CONICET; Argentina.Fil: Andreatta, Alfonsina E. UTN Fac. Reg. San Francisco, Ingeniería de Procesos Sustentables - CONICET; Argentina.Fil: Verardo, Vito. University of Granada. Department of Nutrition and Food Science; Spain.RICIFA2024-03-26T19:02:54Z2024-03-26T19:02:54Z2023-11-16info:eu-repo/semantics/conferenceObjectinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciapdfapplication/pdfEnhancement of Extraction Parameters for Bioactive Substances from Ocimum basilicum Leaves through Sonotrode-Assisted Ultrasonic Extraction. Carolina Aloisio; María del Carmen Razola-Díaz; María José Aznar-Ramos; Marcela R. Longhi; Alfonsina E. Andreatta; Vito Verardo. Rosario. 7ma Reunión Internacional de Ciencias Farmacéuticas (RICiFa 2023) 16y 17 de noviembre de 2023.https://ricifa.com.ar/http://hdl.handle.net/20.500.12272/10119engengNacionalinfo:eu-repo/semantics/openAccess2024-03-26T19:02:54Zhttp://creativecommons.org/licenses/by-nc-nd/4.0/Attribution-NonCommercial-NoDerivatives 4.0 Internacional.reponame:Repositorio Institucional Abierto (UTN)instname:Universidad Tecnológica Nacional2026-09-24T12:46:56Zoai:ria.utn.edu.ar:20.500.12272/10119instacron:UTNInstitucionalhttp://ria.utn.edu.ar/Universidad públicaNo correspondehttp://ria.utn.edu.ar/oaigestionria@rec.utn.edu.ar; fsuarez@rec.utn.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2026-09-24 12:46:57.329Repositorio Institucional Abierto (UTN) - Universidad Tecnológica Nacionalfalse
dc.title.none.fl_str_mv Enhancement of extraction parameters for bioactive substances from ocimum basilicum leaves through sonotrode-assisted ultrasonic extraction
title Enhancement of extraction parameters for bioactive substances from ocimum basilicum leaves through sonotrode-assisted ultrasonic extraction
spellingShingle Enhancement of extraction parameters for bioactive substances from ocimum basilicum leaves through sonotrode-assisted ultrasonic extraction
Aloisio, Carolina
Ocimum basilicum
Ultrasonic Extraction
Optimization
title_short Enhancement of extraction parameters for bioactive substances from ocimum basilicum leaves through sonotrode-assisted ultrasonic extraction
title_full Enhancement of extraction parameters for bioactive substances from ocimum basilicum leaves through sonotrode-assisted ultrasonic extraction
title_fullStr Enhancement of extraction parameters for bioactive substances from ocimum basilicum leaves through sonotrode-assisted ultrasonic extraction
title_full_unstemmed Enhancement of extraction parameters for bioactive substances from ocimum basilicum leaves through sonotrode-assisted ultrasonic extraction
title_sort Enhancement of extraction parameters for bioactive substances from ocimum basilicum leaves through sonotrode-assisted ultrasonic extraction
dc.creator.none.fl_str_mv Aloisio, Carolina
Razola-Díaz, María del Carmen
Aznar-Ramos, María José
Longhi, Marcela
Andreatta, Alfonsina E.
Verardo, Vito
author Aloisio, Carolina
author_facet Aloisio, Carolina
Razola-Díaz, María del Carmen
Aznar-Ramos, María José
Longhi, Marcela
Andreatta, Alfonsina E.
Verardo, Vito
author_role author
author2 Razola-Díaz, María del Carmen
Aznar-Ramos, María José
Longhi, Marcela
Andreatta, Alfonsina E.
Verardo, Vito
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Ocimum basilicum
Ultrasonic Extraction
Optimization
topic Ocimum basilicum
Ultrasonic Extraction
Optimization
dc.description.none.fl_txt_mv Sweet basil (Ocimum basilicum) leaves are rich in bioactive compounds that present therapeutical benefits for human health. Ultrasonic-assisted extraction (UAE) is frequently used to obtain phenolic compounds in plants. However, few works have developed multivariable studies to find the optimal conditions to extract the maximum amount of compounds, especially applied to UAE via sonotrode. The purpose of this work was to perform a multi-variable study employing a Box–Behnken design to collect the highest active compound content from Ocimum basilicum leaves. The efficacy of the design was endorsed by ANOVA. The studied parameters for UAE via sonotrode were ethanol/water ratio, amplitude and time. The analyzed responses were the rosmarinic acid, sum of phenolic acids and sum of phenolic compounds content. Optimal conditions were found to be 50% ethanol/water, 50% amplitude and 5 min. 27 bioactive compounds were identified by HPLC-ESI-TOF-MS, when the extract was collected by applying the optimal conditions. Ocimum basilicum may be appreciated as a valuable source of important bioactive substances for pharmaceutical use.
CONICET, UNC, UTN, Universidad de Granada.
Fil: Aloisio, Carolina. UTN Fac. Reg. San Francisco. Ingeniería de Procesos Sustentables - Universidad Nacional de Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica (UNITEFA) - CONICET; Argentina.
Fil: Razola-Díaz, María del Carmen. University of Granada. Department of Nutrition and Food Science; Spain.
Fil: Aznar-Ramos, María José. University of Granada. Department of Nutrition and Food Science; Spain.
Fil: Longhi, Marcela. Universidad Nacional de Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica (UNITEFA) - CONICET; Argentina.
Fil: Andreatta, Alfonsina E. UTN Fac. Reg. San Francisco, Ingeniería de Procesos Sustentables - CONICET; Argentina.
Fil: Verardo, Vito. University of Granada. Department of Nutrition and Food Science; Spain.
description Sweet basil (Ocimum basilicum) leaves are rich in bioactive compounds that present therapeutical benefits for human health. Ultrasonic-assisted extraction (UAE) is frequently used to obtain phenolic compounds in plants. However, few works have developed multivariable studies to find the optimal conditions to extract the maximum amount of compounds, especially applied to UAE via sonotrode. The purpose of this work was to perform a multi-variable study employing a Box–Behnken design to collect the highest active compound content from Ocimum basilicum leaves. The efficacy of the design was endorsed by ANOVA. The studied parameters for UAE via sonotrode were ethanol/water ratio, amplitude and time. The analyzed responses were the rosmarinic acid, sum of phenolic acids and sum of phenolic compounds content. Optimal conditions were found to be 50% ethanol/water, 50% amplitude and 5 min. 27 bioactive compounds were identified by HPLC-ESI-TOF-MS, when the extract was collected by applying the optimal conditions. Ocimum basilicum may be appreciated as a valuable source of important bioactive substances for pharmaceutical use.
publishDate 2023
dc.date.none.fl_str_mv 2023-11-16
2024-03-26T19:02:54Z
2024-03-26T19:02:54Z
dc.type.none.fl_str_mv info:eu-repo/semantics/conferenceObject
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
format conferenceObject
status_str acceptedVersion
dc.identifier.none.fl_str_mv Enhancement of Extraction Parameters for Bioactive Substances from Ocimum basilicum Leaves through Sonotrode-Assisted Ultrasonic Extraction. Carolina Aloisio; María del Carmen Razola-Díaz; María José Aznar-Ramos; Marcela R. Longhi; Alfonsina E. Andreatta; Vito Verardo. Rosario. 7ma Reunión Internacional de Ciencias Farmacéuticas (RICiFa 2023) 16y 17 de noviembre de 2023.
https://ricifa.com.ar/
http://hdl.handle.net/20.500.12272/10119
identifier_str_mv Enhancement of Extraction Parameters for Bioactive Substances from Ocimum basilicum Leaves through Sonotrode-Assisted Ultrasonic Extraction. Carolina Aloisio; María del Carmen Razola-Díaz; María José Aznar-Ramos; Marcela R. Longhi; Alfonsina E. Andreatta; Vito Verardo. Rosario. 7ma Reunión Internacional de Ciencias Farmacéuticas (RICiFa 2023) 16y 17 de noviembre de 2023.
url https://ricifa.com.ar/
http://hdl.handle.net/20.500.12272/10119
dc.language.none.fl_str_mv eng
eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
2024-03-26T19:02:54Z
http://creativecommons.org/licenses/by-nc-nd/4.0/
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
.
eu_rights_str_mv openAccess
rights_invalid_str_mv 2024-03-26T19:02:54Z
http://creativecommons.org/licenses/by-nc-nd/4.0/
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
.
dc.format.none.fl_str_mv pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv RICIFA
publisher.none.fl_str_mv RICIFA
dc.source.none.fl_str_mv reponame:Repositorio Institucional Abierto (UTN)
instname:Universidad Tecnológica Nacional
reponame_str Repositorio Institucional Abierto (UTN)
collection Repositorio Institucional Abierto (UTN)
instname_str Universidad Tecnológica Nacional
repository.name.fl_str_mv Repositorio Institucional Abierto (UTN) - Universidad Tecnológica Nacional
repository.mail.fl_str_mv gestionria@rec.utn.edu.ar; fsuarez@rec.utn.edu.ar
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score 13.24418

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