Optimization of a site-specific biostimulation strategy using response-surface methodology to remediate a chronically hydrocarbon-contaminated industrial soil.

Autores
Conde Molina, Débora; Liporace, Franco; Quevedo, Carla
Año de publicación
2021
Idioma
español castellano
Tipo de recurso
documento de conferencia
Estado
versión aceptada
Descripción
In present times, contamination with petroleum compounds is one of the most important environmental problems, mainly in industrial areas. The Zárate-Campana petrochemical pole, Buenos Aires, Argentina, has a history of hydrocarbon contamination of more than 100 years, so it is relevant to recover these contaminated areas through the development of site-specific bioremediation technologies that are compatible with the environment. In this work, biostimulation strategy to remediate chronically hydrocarbon-contaminated soil was approached by the addition of nutrients such as nitrogen (N) and phosphorus (P) in order to evaluate the influence of these on the growth of the autochthonous microflora. Therefore, the response-surface methodology as a statistical tool was used to predict the optimum values of N and P concentration with the aim of obtaining the maximum total hydrocarbon removal in the soil. Microcosm systems were carried out into flasks containing contaminated samples belonging to RHASA refinery areas. Different N (NaNO3) and P (Na2HPO4) concentrations were added to each system according to central composite design, and incubated at 20-25°C for 90 days. Hydrocarbon concentration content was measured by gas chromatography as the response of the model. Results showed that the addition of 0.589 g N/kg and 0.304 g P/kg leads to the highest hydrocarbon removal efficiency, decreasing from 6881 ppm to 728 ppm. In addition biostimulation strategy was compared with natural attenuation and 89.71% of total hydrocarbons were removed when the biostimulation was applied, while in natural attenuation was 72.06%. In both treatments, total aerobic heterotrophic bacteria increased during 90 days, whereas the count of hydrocarbon degrading bacteria remained stable. The biostimulation strategy approached in this work showed to be a promising alternative to remediate the soils of the study site.
Fil: Conde Molina, Débora. Universidad Tecnológica Nacional. Facultad Regional Delta. Grupo de Procesos Biotecnológicos; Argentina.
Fil: Liporace, Franco. Universidad Tecnológica Nacional. Facultad Regional Delta. Grupo de Procesos Biotecnológicos; Argentina.
Fil: Quevedo, Carla. Universidad Tecnológica Nacional. Facultad Regional Delta. Grupo de Procesos Biotecnológicos; Argentina.
Materia
UTN
Facultad Regional Delta
Chronically hydrocarbon-contaminated soil
Biostimulation strategy
Optimization
Microscoms
Nivel de accesibilidad
acceso abierto
Condiciones de uso
2022-04-22T17:44:22Z
Repositorio
Repositorio Institucional Abierto (UTN)
Institución
Universidad Tecnológica Nacional
OAI Identificador
oai:ria.utn.edu.ar:20.500.12272/6166
Acceder
id RIAUTN_3408209d0776522d9f9434b9c28c592a
oai_identifier_str oai:ria.utn.edu.ar:20.500.12272/6166
network_acronym_str RIAUTN
repository_id_str a
network_name_str Repositorio Institucional Abierto (UTN)
spelling Optimization of a site-specific biostimulation strategy using response-surface methodology to remediate a chronically hydrocarbon-contaminated industrial soil.Conde Molina, DéboraLiporace, FrancoQuevedo, CarlaUTNFacultad Regional DeltaChronically hydrocarbon-contaminated soilBiostimulation strategyOptimizationMicroscomsIn present times, contamination with petroleum compounds is one of the most important environmental problems, mainly in industrial areas. The Zárate-Campana petrochemical pole, Buenos Aires, Argentina, has a history of hydrocarbon contamination of more than 100 years, so it is relevant to recover these contaminated areas through the development of site-specific bioremediation technologies that are compatible with the environment. In this work, biostimulation strategy to remediate chronically hydrocarbon-contaminated soil was approached by the addition of nutrients such as nitrogen (N) and phosphorus (P) in order to evaluate the influence of these on the growth of the autochthonous microflora. Therefore, the response-surface methodology as a statistical tool was used to predict the optimum values of N and P concentration with the aim of obtaining the maximum total hydrocarbon removal in the soil. Microcosm systems were carried out into flasks containing contaminated samples belonging to RHASA refinery areas. Different N (NaNO3) and P (Na2HPO4) concentrations were added to each system according to central composite design, and incubated at 20-25°C for 90 days. Hydrocarbon concentration content was measured by gas chromatography as the response of the model. Results showed that the addition of 0.589 g N/kg and 0.304 g P/kg leads to the highest hydrocarbon removal efficiency, decreasing from 6881 ppm to 728 ppm. In addition biostimulation strategy was compared with natural attenuation and 89.71% of total hydrocarbons were removed when the biostimulation was applied, while in natural attenuation was 72.06%. In both treatments, total aerobic heterotrophic bacteria increased during 90 days, whereas the count of hydrocarbon degrading bacteria remained stable. The biostimulation strategy approached in this work showed to be a promising alternative to remediate the soils of the study site.Fil: Conde Molina, Débora. Universidad Tecnológica Nacional. Facultad Regional Delta. Grupo de Procesos Biotecnológicos; Argentina.Fil: Liporace, Franco. Universidad Tecnológica Nacional. Facultad Regional Delta. Grupo de Procesos Biotecnológicos; Argentina.Fil: Quevedo, Carla. Universidad Tecnológica Nacional. Facultad Regional Delta. Grupo de Procesos Biotecnológicos; Argentina.Argentine Society for Biochemistry and Molecular Biology2022-04-22T17:44:22Z2022-04-22T17:44:22Z2021-11info:eu-repo/semantics/conferenceObjectinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciapdfapplication/pdfConde Molina D., Liporace F., Quevedo C. Optimization of a site-specific biostimulation strategy using response-surface methodology to remediate a chronically hydrocarbon-contaminated industrial soil. 57th Annual Meeting Argentine Society for Biochemistry and Molecular Biology. Biocell 46 (Suppl. 1), 93.http://hdl.handle.net/20.500.12272/6166spainfo:eu-repo/semantics/openAccess2022-04-22T17:44:22Zhttp://creativecommons.org/licenses/by-nc-sa/4.0/Atribución-NoComercial-CompartirIgual 4.0 InternacionalLos autoresAtribución – No Comercial – Sin Obra Derivada (by-nc-nd)reponame:Repositorio Institucional Abierto (UTN)instname:Universidad Tecnológica Nacional2026-10-01T11:58:27Zoai:ria.utn.edu.ar:20.500.12272/6166instacron:UTNInstitucionalhttp://ria.utn.edu.ar/Universidad públicaNo correspondehttp://ria.utn.edu.ar/oaigestionria@rec.utn.edu.ar; fsuarez@rec.utn.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2026-10-01 11:58:28.292Repositorio Institucional Abierto (UTN) - Universidad Tecnológica Nacionalfalse
dc.title.none.fl_str_mv Optimization of a site-specific biostimulation strategy using response-surface methodology to remediate a chronically hydrocarbon-contaminated industrial soil.
title Optimization of a site-specific biostimulation strategy using response-surface methodology to remediate a chronically hydrocarbon-contaminated industrial soil.
spellingShingle Optimization of a site-specific biostimulation strategy using response-surface methodology to remediate a chronically hydrocarbon-contaminated industrial soil.
Conde Molina, Débora
UTN
Facultad Regional Delta
Chronically hydrocarbon-contaminated soil
Biostimulation strategy
Optimization
Microscoms
title_short Optimization of a site-specific biostimulation strategy using response-surface methodology to remediate a chronically hydrocarbon-contaminated industrial soil.
title_full Optimization of a site-specific biostimulation strategy using response-surface methodology to remediate a chronically hydrocarbon-contaminated industrial soil.
title_fullStr Optimization of a site-specific biostimulation strategy using response-surface methodology to remediate a chronically hydrocarbon-contaminated industrial soil.
title_full_unstemmed Optimization of a site-specific biostimulation strategy using response-surface methodology to remediate a chronically hydrocarbon-contaminated industrial soil.
title_sort Optimization of a site-specific biostimulation strategy using response-surface methodology to remediate a chronically hydrocarbon-contaminated industrial soil.
dc.creator.none.fl_str_mv Conde Molina, Débora
Liporace, Franco
Quevedo, Carla
author Conde Molina, Débora
author_facet Conde Molina, Débora
Liporace, Franco
Quevedo, Carla
author_role author
author2 Liporace, Franco
Quevedo, Carla
author2_role author
author
dc.subject.none.fl_str_mv UTN
Facultad Regional Delta
Chronically hydrocarbon-contaminated soil
Biostimulation strategy
Optimization
Microscoms
topic UTN
Facultad Regional Delta
Chronically hydrocarbon-contaminated soil
Biostimulation strategy
Optimization
Microscoms
dc.description.none.fl_txt_mv In present times, contamination with petroleum compounds is one of the most important environmental problems, mainly in industrial areas. The Zárate-Campana petrochemical pole, Buenos Aires, Argentina, has a history of hydrocarbon contamination of more than 100 years, so it is relevant to recover these contaminated areas through the development of site-specific bioremediation technologies that are compatible with the environment. In this work, biostimulation strategy to remediate chronically hydrocarbon-contaminated soil was approached by the addition of nutrients such as nitrogen (N) and phosphorus (P) in order to evaluate the influence of these on the growth of the autochthonous microflora. Therefore, the response-surface methodology as a statistical tool was used to predict the optimum values of N and P concentration with the aim of obtaining the maximum total hydrocarbon removal in the soil. Microcosm systems were carried out into flasks containing contaminated samples belonging to RHASA refinery areas. Different N (NaNO3) and P (Na2HPO4) concentrations were added to each system according to central composite design, and incubated at 20-25°C for 90 days. Hydrocarbon concentration content was measured by gas chromatography as the response of the model. Results showed that the addition of 0.589 g N/kg and 0.304 g P/kg leads to the highest hydrocarbon removal efficiency, decreasing from 6881 ppm to 728 ppm. In addition biostimulation strategy was compared with natural attenuation and 89.71% of total hydrocarbons were removed when the biostimulation was applied, while in natural attenuation was 72.06%. In both treatments, total aerobic heterotrophic bacteria increased during 90 days, whereas the count of hydrocarbon degrading bacteria remained stable. The biostimulation strategy approached in this work showed to be a promising alternative to remediate the soils of the study site.
Fil: Conde Molina, Débora. Universidad Tecnológica Nacional. Facultad Regional Delta. Grupo de Procesos Biotecnológicos; Argentina.
Fil: Liporace, Franco. Universidad Tecnológica Nacional. Facultad Regional Delta. Grupo de Procesos Biotecnológicos; Argentina.
Fil: Quevedo, Carla. Universidad Tecnológica Nacional. Facultad Regional Delta. Grupo de Procesos Biotecnológicos; Argentina.
description In present times, contamination with petroleum compounds is one of the most important environmental problems, mainly in industrial areas. The Zárate-Campana petrochemical pole, Buenos Aires, Argentina, has a history of hydrocarbon contamination of more than 100 years, so it is relevant to recover these contaminated areas through the development of site-specific bioremediation technologies that are compatible with the environment. In this work, biostimulation strategy to remediate chronically hydrocarbon-contaminated soil was approached by the addition of nutrients such as nitrogen (N) and phosphorus (P) in order to evaluate the influence of these on the growth of the autochthonous microflora. Therefore, the response-surface methodology as a statistical tool was used to predict the optimum values of N and P concentration with the aim of obtaining the maximum total hydrocarbon removal in the soil. Microcosm systems were carried out into flasks containing contaminated samples belonging to RHASA refinery areas. Different N (NaNO3) and P (Na2HPO4) concentrations were added to each system according to central composite design, and incubated at 20-25°C for 90 days. Hydrocarbon concentration content was measured by gas chromatography as the response of the model. Results showed that the addition of 0.589 g N/kg and 0.304 g P/kg leads to the highest hydrocarbon removal efficiency, decreasing from 6881 ppm to 728 ppm. In addition biostimulation strategy was compared with natural attenuation and 89.71% of total hydrocarbons were removed when the biostimulation was applied, while in natural attenuation was 72.06%. In both treatments, total aerobic heterotrophic bacteria increased during 90 days, whereas the count of hydrocarbon degrading bacteria remained stable. The biostimulation strategy approached in this work showed to be a promising alternative to remediate the soils of the study site.
publishDate 2021
dc.date.none.fl_str_mv 2021-11
2022-04-22T17:44:22Z
2022-04-22T17:44:22Z
dc.type.none.fl_str_mv info:eu-repo/semantics/conferenceObject
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
format conferenceObject
status_str acceptedVersion
dc.identifier.none.fl_str_mv Conde Molina D., Liporace F., Quevedo C. Optimization of a site-specific biostimulation strategy using response-surface methodology to remediate a chronically hydrocarbon-contaminated industrial soil. 57th Annual Meeting Argentine Society for Biochemistry and Molecular Biology. Biocell 46 (Suppl. 1), 93.
http://hdl.handle.net/20.500.12272/6166
identifier_str_mv Conde Molina D., Liporace F., Quevedo C. Optimization of a site-specific biostimulation strategy using response-surface methodology to remediate a chronically hydrocarbon-contaminated industrial soil. 57th Annual Meeting Argentine Society for Biochemistry and Molecular Biology. Biocell 46 (Suppl. 1), 93.
url http://hdl.handle.net/20.500.12272/6166
dc.language.none.fl_str_mv spa
language spa
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
2022-04-22T17:44:22Z
http://creativecommons.org/licenses/by-nc-sa/4.0/
Atribución-NoComercial-CompartirIgual 4.0 Internacional
Los autores
Atribución – No Comercial – Sin Obra Derivada (by-nc-nd)
eu_rights_str_mv openAccess
rights_invalid_str_mv 2022-04-22T17:44:22Z
http://creativecommons.org/licenses/by-nc-sa/4.0/
Atribución-NoComercial-CompartirIgual 4.0 Internacional
Los autores
Atribución – No Comercial – Sin Obra Derivada (by-nc-nd)
dc.format.none.fl_str_mv pdf
application/pdf
dc.publisher.none.fl_str_mv Argentine Society for Biochemistry and Molecular Biology
publisher.none.fl_str_mv Argentine Society for Biochemistry and Molecular Biology
dc.source.none.fl_str_mv reponame:Repositorio Institucional Abierto (UTN)
instname:Universidad Tecnológica Nacional
reponame_str Repositorio Institucional Abierto (UTN)
collection Repositorio Institucional Abierto (UTN)
instname_str Universidad Tecnológica Nacional
repository.name.fl_str_mv Repositorio Institucional Abierto (UTN) - Universidad Tecnológica Nacional
repository.mail.fl_str_mv gestionria@rec.utn.edu.ar; fsuarez@rec.utn.edu.ar
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score 13.365483

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