Digestive proteinases from the marine fish processing wastes of the South‐West Atlantic ocean: their partial characterization and comparison.

Autores
Friedman, Ivana S.; Behrens, Leonel A.; Pereira, Nair de los Angeles; Contreras, Edgardo M.; Fernández-Gimenez, Analia V.
Año de publicación
2022
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión corregida
Descripción
Fish processing generates plenty of waste that is directly discarded in open-air dumps and water sources, or treated in the same way as urban solid waste, causing serious pollution problems. The waste represents a significant source of high-value bioproducts with potential applications in different industrial processes such as the production of feed, fertilizers, biodiesel and biogas, detergent additives and cosmetics. The objective of this study was to characterize and compare specific activities under different pH values and temperature conditions of acid and alkaline proteinases and viscera yield from the following fish species: Argentine hake Merluccius hubbsi, Brazilian flathead Percophis brasiliensis, Brazilian codling Urophycis brasiliensis and Stripped weakfish Cynoscion guatucupa. Individuals were fished off the coast of Mar del Plata (Argentina) by a commercial fleet and the viscera were immediately extracted and kept on ice until use. Stomach proteinases from four species had the highest activity at pH 2, with stability in the range of pH 2–4. The optimum pH was 11.5 from intestinal enzymes of C. guatucupa, M. hubbsi and P. brasiliensis and 9.5 from intestinal enzymes of U. brasiliensis. Alkaline proteinases from all species were highly stable in the range of 7–11.5. The optimum temperature of stomach proteinases from the four species studied were 30 and 50_C, with stability at 10 and 30_C during 150 min. The optimum temperature of intestinal enzymes from the tested species were 50_C with high stability at 10 and 30_C during 150 min. Alkaline proteinase from all species and acid proteinases from C. guatucupa were inactive at 70_C after 150 min, while there was a residual activity lower than 5% at 80_C on preincubated stomach enzymes of M.hubbsi, P. brasiliensis and U. brasiliensis after 5, 10 and 20 min, respectively. Digestive proteinases recovered in this study could be appropriate for technological usage, reducing manufacturing costs, obtaining revenue from fishery wastes, and contributing to the reduction of environmental pollution.
Fil: Friedman, Ivana S. Universidad Nacional de Mar del Plata. Instituto de Investigaciones Marinas y Costeras, Facultad de Ciencias Exactas y Naturales; Argentina.
Fil: Behrens, Leonel A. Universidad Nacional de Mar del Plata. Instituto de Investigaciones Marinas y Costeras, Facultad de Ciencias Exactas y Naturales; Argentina.
Fil: Pereira, Nair de los Angeles. Universidad Nacional de Mar del Plata. Instituto de Investigaciones Marinas y Costeras, Facultad de Ciencias Exactas y Naturales; Argentina.
Fil: Fernández-Gimenez, Analia V.. Universidad Nacional de Mar del Plata. Instituto de Investigaciones Marinas y Costeras, Facultad de Ciencias Exactas y Naturales; Argentina.
Fil: Contreras, Edgardo M. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ciencia y Tecnología de Materiales, CCT; Argentina.
Materia
Viscera
Licenciatura en acuicultura y producción pesquera
South-west Atlantic ocean
Partial characterization
Fish wastes
Digestive proteinases
Nivel de accesibilidad
acceso abierto
Condiciones de uso
2022-05-20T23:40:49Z
Repositorio
Repositorio Institucional Abierto (UTN)
Institución
Universidad Tecnológica Nacional
OAI Identificador
oai:ria.utn.edu.ar:20.500.12272/6405
Acceder
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network_acronym_str RIAUTN
repository_id_str a
network_name_str Repositorio Institucional Abierto (UTN)
spelling Digestive proteinases from the marine fish processing wastes of the South‐West Atlantic ocean: their partial characterization and comparison.Friedman, Ivana S.Behrens, Leonel A.Pereira, Nair de los AngelesContreras, Edgardo M.Fernández-Gimenez, Analia V.VisceraLicenciatura en acuicultura y producción pesqueraSouth-west Atlantic oceanPartial characterizationFish wastesDigestive proteinasesFish processing generates plenty of waste that is directly discarded in open-air dumps and water sources, or treated in the same way as urban solid waste, causing serious pollution problems. The waste represents a significant source of high-value bioproducts with potential applications in different industrial processes such as the production of feed, fertilizers, biodiesel and biogas, detergent additives and cosmetics. The objective of this study was to characterize and compare specific activities under different pH values and temperature conditions of acid and alkaline proteinases and viscera yield from the following fish species: Argentine hake Merluccius hubbsi, Brazilian flathead Percophis brasiliensis, Brazilian codling Urophycis brasiliensis and Stripped weakfish Cynoscion guatucupa. Individuals were fished off the coast of Mar del Plata (Argentina) by a commercial fleet and the viscera were immediately extracted and kept on ice until use. Stomach proteinases from four species had the highest activity at pH 2, with stability in the range of pH 2–4. The optimum pH was 11.5 from intestinal enzymes of C. guatucupa, M. hubbsi and P. brasiliensis and 9.5 from intestinal enzymes of U. brasiliensis. Alkaline proteinases from all species were highly stable in the range of 7–11.5. The optimum temperature of stomach proteinases from the four species studied were 30 and 50_C, with stability at 10 and 30_C during 150 min. The optimum temperature of intestinal enzymes from the tested species were 50_C with high stability at 10 and 30_C during 150 min. Alkaline proteinase from all species and acid proteinases from C. guatucupa were inactive at 70_C after 150 min, while there was a residual activity lower than 5% at 80_C on preincubated stomach enzymes of M.hubbsi, P. brasiliensis and U. brasiliensis after 5, 10 and 20 min, respectively. Digestive proteinases recovered in this study could be appropriate for technological usage, reducing manufacturing costs, obtaining revenue from fishery wastes, and contributing to the reduction of environmental pollution.Fil: Friedman, Ivana S. Universidad Nacional de Mar del Plata. Instituto de Investigaciones Marinas y Costeras, Facultad de Ciencias Exactas y Naturales; Argentina.Fil: Behrens, Leonel A. Universidad Nacional de Mar del Plata. Instituto de Investigaciones Marinas y Costeras, Facultad de Ciencias Exactas y Naturales; Argentina.Fil: Pereira, Nair de los Angeles. Universidad Nacional de Mar del Plata. Instituto de Investigaciones Marinas y Costeras, Facultad de Ciencias Exactas y Naturales; Argentina.Fil: Fernández-Gimenez, Analia V.. Universidad Nacional de Mar del Plata. Instituto de Investigaciones Marinas y Costeras, Facultad de Ciencias Exactas y Naturales; Argentina.Fil: Contreras, Edgardo M. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ciencia y Tecnología de Materiales, CCT; Argentina.2022-05-20T23:40:49Z2022-05-20T23:40:49Z2022-01-01info:eu-repo/semantics/conferenceObjectinfo:eu-repo/semantics/updatedVersionhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciapdfapplication/pdfFriedman, I. S.; Behrens, L. A.; Pereira, N. D. A.; Contreras, E. M.; Fernández‐Gimenez, A. V. (2022) Digestive proteinases from the marine fish processing wastes of the South‐West Atlantic Ocean: Their partial characterization and comparison. Journal of Fish Biology, 100 (1), 150-160. https://onlinelibrary.wiley.com/doi/abs/10.1111/jfb.14929https://onlinelibrary.wiley.com/doi/abs/10.1111/jfb.14929http://hdl.handle.net/20.500.12272/6405DOI: 10.1111/jfb.14929enginfo:eu-repo/semantics/openAccess2022-05-20T23:40:49Zhttp://creativecommons.org/licenses/by-nc/4.0/Atribución-NoComercial 4.0 InternacionalReservados todos los derechos. No se permite la reproducción total o parcial de esta obra, ni su incorporación a un sistema informático, ni su transmisión en cualquier forma o por cualquier medio (electrónico, mecánico, fotocopia, grabación u otros) sin autorización previa y por escrito de los titulares del copyright. La infracción de dichos derechos puede constituir un delito contra la propiedad intelectual.reponame:Repositorio Institucional Abierto (UTN)instname:Universidad Tecnológica Nacional2026-09-24T12:48:37Zoai:ria.utn.edu.ar:20.500.12272/6405instacron:UTNInstitucionalhttp://ria.utn.edu.ar/Universidad públicaNo correspondehttp://ria.utn.edu.ar/oaigestionria@rec.utn.edu.ar; fsuarez@rec.utn.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2026-09-24 12:48:39.01Repositorio Institucional Abierto (UTN) - Universidad Tecnológica Nacionalfalse
dc.title.none.fl_str_mv Digestive proteinases from the marine fish processing wastes of the South‐West Atlantic ocean: their partial characterization and comparison.
title Digestive proteinases from the marine fish processing wastes of the South‐West Atlantic ocean: their partial characterization and comparison.
spellingShingle Digestive proteinases from the marine fish processing wastes of the South‐West Atlantic ocean: their partial characterization and comparison.
Friedman, Ivana S.
Viscera
Licenciatura en acuicultura y producción pesquera
South-west Atlantic ocean
Partial characterization
Fish wastes
Digestive proteinases
title_short Digestive proteinases from the marine fish processing wastes of the South‐West Atlantic ocean: their partial characterization and comparison.
title_full Digestive proteinases from the marine fish processing wastes of the South‐West Atlantic ocean: their partial characterization and comparison.
title_fullStr Digestive proteinases from the marine fish processing wastes of the South‐West Atlantic ocean: their partial characterization and comparison.
title_full_unstemmed Digestive proteinases from the marine fish processing wastes of the South‐West Atlantic ocean: their partial characterization and comparison.
title_sort Digestive proteinases from the marine fish processing wastes of the South‐West Atlantic ocean: their partial characterization and comparison.
dc.creator.none.fl_str_mv Friedman, Ivana S.
Behrens, Leonel A.
Pereira, Nair de los Angeles
Contreras, Edgardo M.
Fernández-Gimenez, Analia V.
author Friedman, Ivana S.
author_facet Friedman, Ivana S.
Behrens, Leonel A.
Pereira, Nair de los Angeles
Contreras, Edgardo M.
Fernández-Gimenez, Analia V.
author_role author
author2 Behrens, Leonel A.
Pereira, Nair de los Angeles
Contreras, Edgardo M.
Fernández-Gimenez, Analia V.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Viscera
Licenciatura en acuicultura y producción pesquera
South-west Atlantic ocean
Partial characterization
Fish wastes
Digestive proteinases
topic Viscera
Licenciatura en acuicultura y producción pesquera
South-west Atlantic ocean
Partial characterization
Fish wastes
Digestive proteinases
dc.description.none.fl_txt_mv Fish processing generates plenty of waste that is directly discarded in open-air dumps and water sources, or treated in the same way as urban solid waste, causing serious pollution problems. The waste represents a significant source of high-value bioproducts with potential applications in different industrial processes such as the production of feed, fertilizers, biodiesel and biogas, detergent additives and cosmetics. The objective of this study was to characterize and compare specific activities under different pH values and temperature conditions of acid and alkaline proteinases and viscera yield from the following fish species: Argentine hake Merluccius hubbsi, Brazilian flathead Percophis brasiliensis, Brazilian codling Urophycis brasiliensis and Stripped weakfish Cynoscion guatucupa. Individuals were fished off the coast of Mar del Plata (Argentina) by a commercial fleet and the viscera were immediately extracted and kept on ice until use. Stomach proteinases from four species had the highest activity at pH 2, with stability in the range of pH 2–4. The optimum pH was 11.5 from intestinal enzymes of C. guatucupa, M. hubbsi and P. brasiliensis and 9.5 from intestinal enzymes of U. brasiliensis. Alkaline proteinases from all species were highly stable in the range of 7–11.5. The optimum temperature of stomach proteinases from the four species studied were 30 and 50_C, with stability at 10 and 30_C during 150 min. The optimum temperature of intestinal enzymes from the tested species were 50_C with high stability at 10 and 30_C during 150 min. Alkaline proteinase from all species and acid proteinases from C. guatucupa were inactive at 70_C after 150 min, while there was a residual activity lower than 5% at 80_C on preincubated stomach enzymes of M.hubbsi, P. brasiliensis and U. brasiliensis after 5, 10 and 20 min, respectively. Digestive proteinases recovered in this study could be appropriate for technological usage, reducing manufacturing costs, obtaining revenue from fishery wastes, and contributing to the reduction of environmental pollution.
Fil: Friedman, Ivana S. Universidad Nacional de Mar del Plata. Instituto de Investigaciones Marinas y Costeras, Facultad de Ciencias Exactas y Naturales; Argentina.
Fil: Behrens, Leonel A. Universidad Nacional de Mar del Plata. Instituto de Investigaciones Marinas y Costeras, Facultad de Ciencias Exactas y Naturales; Argentina.
Fil: Pereira, Nair de los Angeles. Universidad Nacional de Mar del Plata. Instituto de Investigaciones Marinas y Costeras, Facultad de Ciencias Exactas y Naturales; Argentina.
Fil: Fernández-Gimenez, Analia V.. Universidad Nacional de Mar del Plata. Instituto de Investigaciones Marinas y Costeras, Facultad de Ciencias Exactas y Naturales; Argentina.
Fil: Contreras, Edgardo M. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ciencia y Tecnología de Materiales, CCT; Argentina.
description Fish processing generates plenty of waste that is directly discarded in open-air dumps and water sources, or treated in the same way as urban solid waste, causing serious pollution problems. The waste represents a significant source of high-value bioproducts with potential applications in different industrial processes such as the production of feed, fertilizers, biodiesel and biogas, detergent additives and cosmetics. The objective of this study was to characterize and compare specific activities under different pH values and temperature conditions of acid and alkaline proteinases and viscera yield from the following fish species: Argentine hake Merluccius hubbsi, Brazilian flathead Percophis brasiliensis, Brazilian codling Urophycis brasiliensis and Stripped weakfish Cynoscion guatucupa. Individuals were fished off the coast of Mar del Plata (Argentina) by a commercial fleet and the viscera were immediately extracted and kept on ice until use. Stomach proteinases from four species had the highest activity at pH 2, with stability in the range of pH 2–4. The optimum pH was 11.5 from intestinal enzymes of C. guatucupa, M. hubbsi and P. brasiliensis and 9.5 from intestinal enzymes of U. brasiliensis. Alkaline proteinases from all species were highly stable in the range of 7–11.5. The optimum temperature of stomach proteinases from the four species studied were 30 and 50_C, with stability at 10 and 30_C during 150 min. The optimum temperature of intestinal enzymes from the tested species were 50_C with high stability at 10 and 30_C during 150 min. Alkaline proteinase from all species and acid proteinases from C. guatucupa were inactive at 70_C after 150 min, while there was a residual activity lower than 5% at 80_C on preincubated stomach enzymes of M.hubbsi, P. brasiliensis and U. brasiliensis after 5, 10 and 20 min, respectively. Digestive proteinases recovered in this study could be appropriate for technological usage, reducing manufacturing costs, obtaining revenue from fishery wastes, and contributing to the reduction of environmental pollution.
publishDate 2022
dc.date.none.fl_str_mv 2022-05-20T23:40:49Z
2022-05-20T23:40:49Z
2022-01-01
dc.type.none.fl_str_mv info:eu-repo/semantics/conferenceObject
info:eu-repo/semantics/updatedVersion
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
format conferenceObject
status_str updatedVersion
dc.identifier.none.fl_str_mv Friedman, I. S.; Behrens, L. A.; Pereira, N. D. A.; Contreras, E. M.; Fernández‐Gimenez, A. V. (2022) Digestive proteinases from the marine fish processing wastes of the South‐West Atlantic Ocean: Their partial characterization and comparison. Journal of Fish Biology, 100 (1), 150-160. https://onlinelibrary.wiley.com/doi/abs/10.1111/jfb.14929
https://onlinelibrary.wiley.com/doi/abs/10.1111/jfb.14929
http://hdl.handle.net/20.500.12272/6405
DOI: 10.1111/jfb.14929
identifier_str_mv Friedman, I. S.; Behrens, L. A.; Pereira, N. D. A.; Contreras, E. M.; Fernández‐Gimenez, A. V. (2022) Digestive proteinases from the marine fish processing wastes of the South‐West Atlantic Ocean: Their partial characterization and comparison. Journal of Fish Biology, 100 (1), 150-160. https://onlinelibrary.wiley.com/doi/abs/10.1111/jfb.14929
DOI: 10.1111/jfb.14929
url https://onlinelibrary.wiley.com/doi/abs/10.1111/jfb.14929
http://hdl.handle.net/20.500.12272/6405
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
2022-05-20T23:40:49Z
http://creativecommons.org/licenses/by-nc/4.0/
Atribución-NoComercial 4.0 Internacional
Reservados todos los derechos. No se permite la reproducción total o parcial de esta obra, ni su incorporación a un sistema informático, ni su transmisión en cualquier forma o por cualquier medio (electrónico, mecánico, fotocopia, grabación u otros) sin autorización previa y por escrito de los titulares del copyright. La infracción de dichos derechos puede constituir un delito contra la propiedad intelectual.
eu_rights_str_mv openAccess
rights_invalid_str_mv 2022-05-20T23:40:49Z
http://creativecommons.org/licenses/by-nc/4.0/
Atribución-NoComercial 4.0 Internacional
Reservados todos los derechos. No se permite la reproducción total o parcial de esta obra, ni su incorporación a un sistema informático, ni su transmisión en cualquier forma o por cualquier medio (electrónico, mecánico, fotocopia, grabación u otros) sin autorización previa y por escrito de los titulares del copyright. La infracción de dichos derechos puede constituir un delito contra la propiedad intelectual.
dc.format.none.fl_str_mv pdf
application/pdf
dc.source.none.fl_str_mv reponame:Repositorio Institucional Abierto (UTN)
instname:Universidad Tecnológica Nacional
reponame_str Repositorio Institucional Abierto (UTN)
collection Repositorio Institucional Abierto (UTN)
instname_str Universidad Tecnológica Nacional
repository.name.fl_str_mv Repositorio Institucional Abierto (UTN) - Universidad Tecnológica Nacional
repository.mail.fl_str_mv gestionria@rec.utn.edu.ar; fsuarez@rec.utn.edu.ar
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