Clomazone biodegradation in soil microcosms : evaluation of bioaugmentation and biostimulation with native bacteria from rice crop soils in Chaco, Argentina

Autores
Cuadra, Pablo Nicolás; Farías, Alejandro Rubén; Echeverría, Macarena Celeste; Jorge, Nelly Lidia; Vullo, Diana Lia
Año de publicación
2025
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Clomazone (CMZ) is a selective pre and post emergence herbicide indicated for application in rice crops. Its incorrect use has been causing environmental problems, such as soil, groundwater and surface water persistent contamination. CMZ-resistant/tolerant native bacterial strains were previously isolated from rice crop soils in Las Palmas, Chaco, which were able to degrade this herbicide in batch cultures. The aim of this study was to evaluate CMZ biodegradation under controlled experiments in soil microcosms (MC) by bioaugmentation (the addition of specific microorganisms) and biostimulation (the addition of nutrients to increase microbial population activity) procedures. For this purpose, 30 MC were prepared by triplicates using 150 g of sterile and non-sterile rice crop soils under different treatments: control (non-treated MC), bioaugmentation, and a combination of bioaugmentation and biostimulation. The soil used exhibited a phosphorus deficit (8.4x10-4%), therefore biostimulated MC were designed by adding a stock solution of K2HPO4 1M to achieve the optimal C:N:P ratio of 100:10:1. For bioaugmented-MC, inocula consisted of strain G1 or a mixed culture of the isolates G1, H2, and H3, selected according to previous results. The MC were incubated at 30 °C and monitored for a period of 60 days. Bacterial growth was assessed by viable cell counts every 7 days, while CMZ concentration in soil was quantified by HPLC at regular intervals to determine CMZ removal. Results indicated that sterile soil control MC showed no detectable microbial growth until day 15, and CMZ concentration remained stable throughout the 60 days, confirming its abiotic stability under these conditions. In contrast, the other experimental conditions evidenced bacterial growth over time however no CMZ removal was registered in non sterile soil control MC. In G1 MC, G1 attained a maximum growth of 1×102 CFU/g soil at 14 days and of 1×103 CFU/g soil in P-supplemented MC, suggesting that P availability is a limiting factor. Furthermore, the G1-H3-H2-bioaugmented MC also achieved cell development of 1×103 CFU/g soil at 14 days, both with and without P-amendment indicating a greater intrinsic growth capacity of the consortium and a lesser dependence on external P supplementation. A clear predominance of isolate H2 was observed, followed by H3 and, to a lesser extent, G1, suggesting a dynamic of competition among the strains. Regarding the CMZ concentration analysis by HPLC, differentiated results were obtained: G1-MC did not show significant agrochemical degradation under any treatment condition. However, in the G1-H3-H2-MC a 14% CMZ removal was observed at 35 days without biostimulation, while in P-supplemented MC, the removal percentage increased to 26% at 35 days. These results suggest that the microbial consortium with P-amendment is effective in CMZ degradation in MC, being a factor to consider for the herbicide removal in polluted and productive soils.
Fil: Cuadra, Pablo Nicolás. Universidad Tecnológica Nacional. Facultad Regional Resistencia. Grupo de Investigación Sobre Temas Ambientales y Químicos; Argentina.
Fil: Farías, Alejandro Rubén. Universidad Tecnológica Nacional. Facultad Regional Resistencia. Grupo de Investigación Sobre Temas Ambientales y Químicos; Argentina.
Fil: Echeverría, Macarena Celeste. Universidad Tecnológica Nacional. Facultad Regional Resistencia. Grupo de Investigación Sobre Temas Ambientales y Químicos; Argentina.
Fil: Jorge, Nelly Lidia. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura. Laboratorio de Investigaciones en Tecnología Ambiental; Argentina.
Fil: Vullo, Diana Lia. Universidad Nacional General Sarmiento. Instituto de Ciencias. Área de Química; Argentina.
Materia
clomazone
microcosms
biodegradation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
Acceso abierto
Repositorio
Repositorio Institucional Abierto (UTN)
Institución
Universidad Tecnológica Nacional
OAI Identificador
oai:ria.utn.edu.ar:20.500.12272/14396
Acceder
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oai_identifier_str oai:ria.utn.edu.ar:20.500.12272/14396
network_acronym_str RIAUTN
repository_id_str a
network_name_str Repositorio Institucional Abierto (UTN)
spelling Clomazone biodegradation in soil microcosms : evaluation of bioaugmentation and biostimulation with native bacteria from rice crop soils in Chaco, ArgentinaCuadra, Pablo NicolásFarías, Alejandro RubénEcheverría, Macarena CelesteJorge, Nelly LidiaVullo, Diana LiaclomazonemicrocosmsbiodegradationClomazone (CMZ) is a selective pre and post emergence herbicide indicated for application in rice crops. Its incorrect use has been causing environmental problems, such as soil, groundwater and surface water persistent contamination. CMZ-resistant/tolerant native bacterial strains were previously isolated from rice crop soils in Las Palmas, Chaco, which were able to degrade this herbicide in batch cultures. The aim of this study was to evaluate CMZ biodegradation under controlled experiments in soil microcosms (MC) by bioaugmentation (the addition of specific microorganisms) and biostimulation (the addition of nutrients to increase microbial population activity) procedures. For this purpose, 30 MC were prepared by triplicates using 150 g of sterile and non-sterile rice crop soils under different treatments: control (non-treated MC), bioaugmentation, and a combination of bioaugmentation and biostimulation. The soil used exhibited a phosphorus deficit (8.4x10-4%), therefore biostimulated MC were designed by adding a stock solution of K2HPO4 1M to achieve the optimal C:N:P ratio of 100:10:1. For bioaugmented-MC, inocula consisted of strain G1 or a mixed culture of the isolates G1, H2, and H3, selected according to previous results. The MC were incubated at 30 °C and monitored for a period of 60 days. Bacterial growth was assessed by viable cell counts every 7 days, while CMZ concentration in soil was quantified by HPLC at regular intervals to determine CMZ removal. Results indicated that sterile soil control MC showed no detectable microbial growth until day 15, and CMZ concentration remained stable throughout the 60 days, confirming its abiotic stability under these conditions. In contrast, the other experimental conditions evidenced bacterial growth over time however no CMZ removal was registered in non sterile soil control MC. In G1 MC, G1 attained a maximum growth of 1×102 CFU/g soil at 14 days and of 1×103 CFU/g soil in P-supplemented MC, suggesting that P availability is a limiting factor. Furthermore, the G1-H3-H2-bioaugmented MC also achieved cell development of 1×103 CFU/g soil at 14 days, both with and without P-amendment indicating a greater intrinsic growth capacity of the consortium and a lesser dependence on external P supplementation. A clear predominance of isolate H2 was observed, followed by H3 and, to a lesser extent, G1, suggesting a dynamic of competition among the strains. Regarding the CMZ concentration analysis by HPLC, differentiated results were obtained: G1-MC did not show significant agrochemical degradation under any treatment condition. However, in the G1-H3-H2-MC a 14% CMZ removal was observed at 35 days without biostimulation, while in P-supplemented MC, the removal percentage increased to 26% at 35 days. These results suggest that the microbial consortium with P-amendment is effective in CMZ degradation in MC, being a factor to consider for the herbicide removal in polluted and productive soils.Fil: Cuadra, Pablo Nicolás. Universidad Tecnológica Nacional. Facultad Regional Resistencia. Grupo de Investigación Sobre Temas Ambientales y Químicos; Argentina.Fil: Farías, Alejandro Rubén. Universidad Tecnológica Nacional. Facultad Regional Resistencia. Grupo de Investigación Sobre Temas Ambientales y Químicos; Argentina.Fil: Echeverría, Macarena Celeste. Universidad Tecnológica Nacional. Facultad Regional Resistencia. Grupo de Investigación Sobre Temas Ambientales y Químicos; Argentina.Fil: Jorge, Nelly Lidia. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura. Laboratorio de Investigaciones en Tecnología Ambiental; Argentina.Fil: Vullo, Diana Lia. Universidad Nacional General Sarmiento. Instituto de Ciencias. Área de Química; Argentina.Sociedad Argentina de Microbiología General2025-12-19T18:55:29Z2025-12-02info:eu-repo/semantics/conferenceObjectinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciapdfapplication/pdfXX Congreso Anual de la Sociedad Argentina de Microbiología General (SAMIGE 2025)https://samige.org.ar/https://hdl.handle.net/20.500.12272/14396engMSTCRE0008635TCBiodegradación de herbicidas a nivel de microcosmos mediada por microorganismos nativos de suelos de cultivos de arrozinfo:eu-repo/semantics/openAccessAcceso abiertoreponame:Repositorio Institucional Abierto (UTN)instname:Universidad Tecnológica Nacional2026-09-24T12:46:51Zoai:ria.utn.edu.ar:20.500.12272/14396instacron:UTNInstitucionalhttp://ria.utn.edu.ar/Universidad públicaNo correspondehttp://ria.utn.edu.ar/oaigestionria@rec.utn.edu.ar; fsuarez@rec.utn.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2026-09-24 12:46:51.974Repositorio Institucional Abierto (UTN) - Universidad Tecnológica Nacionalfalse
dc.title.none.fl_str_mv Clomazone biodegradation in soil microcosms : evaluation of bioaugmentation and biostimulation with native bacteria from rice crop soils in Chaco, Argentina
title Clomazone biodegradation in soil microcosms : evaluation of bioaugmentation and biostimulation with native bacteria from rice crop soils in Chaco, Argentina
spellingShingle Clomazone biodegradation in soil microcosms : evaluation of bioaugmentation and biostimulation with native bacteria from rice crop soils in Chaco, Argentina
Cuadra, Pablo Nicolás
clomazone
microcosms
biodegradation
title_short Clomazone biodegradation in soil microcosms : evaluation of bioaugmentation and biostimulation with native bacteria from rice crop soils in Chaco, Argentina
title_full Clomazone biodegradation in soil microcosms : evaluation of bioaugmentation and biostimulation with native bacteria from rice crop soils in Chaco, Argentina
title_fullStr Clomazone biodegradation in soil microcosms : evaluation of bioaugmentation and biostimulation with native bacteria from rice crop soils in Chaco, Argentina
title_full_unstemmed Clomazone biodegradation in soil microcosms : evaluation of bioaugmentation and biostimulation with native bacteria from rice crop soils in Chaco, Argentina
title_sort Clomazone biodegradation in soil microcosms : evaluation of bioaugmentation and biostimulation with native bacteria from rice crop soils in Chaco, Argentina
dc.creator.none.fl_str_mv Cuadra, Pablo Nicolás
Farías, Alejandro Rubén
Echeverría, Macarena Celeste
Jorge, Nelly Lidia
Vullo, Diana Lia
author Cuadra, Pablo Nicolás
author_facet Cuadra, Pablo Nicolás
Farías, Alejandro Rubén
Echeverría, Macarena Celeste
Jorge, Nelly Lidia
Vullo, Diana Lia
author_role author
author2 Farías, Alejandro Rubén
Echeverría, Macarena Celeste
Jorge, Nelly Lidia
Vullo, Diana Lia
author2_role author
author
author
author
dc.subject.none.fl_str_mv clomazone
microcosms
biodegradation
topic clomazone
microcosms
biodegradation
dc.description.none.fl_txt_mv Clomazone (CMZ) is a selective pre and post emergence herbicide indicated for application in rice crops. Its incorrect use has been causing environmental problems, such as soil, groundwater and surface water persistent contamination. CMZ-resistant/tolerant native bacterial strains were previously isolated from rice crop soils in Las Palmas, Chaco, which were able to degrade this herbicide in batch cultures. The aim of this study was to evaluate CMZ biodegradation under controlled experiments in soil microcosms (MC) by bioaugmentation (the addition of specific microorganisms) and biostimulation (the addition of nutrients to increase microbial population activity) procedures. For this purpose, 30 MC were prepared by triplicates using 150 g of sterile and non-sterile rice crop soils under different treatments: control (non-treated MC), bioaugmentation, and a combination of bioaugmentation and biostimulation. The soil used exhibited a phosphorus deficit (8.4x10-4%), therefore biostimulated MC were designed by adding a stock solution of K2HPO4 1M to achieve the optimal C:N:P ratio of 100:10:1. For bioaugmented-MC, inocula consisted of strain G1 or a mixed culture of the isolates G1, H2, and H3, selected according to previous results. The MC were incubated at 30 °C and monitored for a period of 60 days. Bacterial growth was assessed by viable cell counts every 7 days, while CMZ concentration in soil was quantified by HPLC at regular intervals to determine CMZ removal. Results indicated that sterile soil control MC showed no detectable microbial growth until day 15, and CMZ concentration remained stable throughout the 60 days, confirming its abiotic stability under these conditions. In contrast, the other experimental conditions evidenced bacterial growth over time however no CMZ removal was registered in non sterile soil control MC. In G1 MC, G1 attained a maximum growth of 1×102 CFU/g soil at 14 days and of 1×103 CFU/g soil in P-supplemented MC, suggesting that P availability is a limiting factor. Furthermore, the G1-H3-H2-bioaugmented MC also achieved cell development of 1×103 CFU/g soil at 14 days, both with and without P-amendment indicating a greater intrinsic growth capacity of the consortium and a lesser dependence on external P supplementation. A clear predominance of isolate H2 was observed, followed by H3 and, to a lesser extent, G1, suggesting a dynamic of competition among the strains. Regarding the CMZ concentration analysis by HPLC, differentiated results were obtained: G1-MC did not show significant agrochemical degradation under any treatment condition. However, in the G1-H3-H2-MC a 14% CMZ removal was observed at 35 days without biostimulation, while in P-supplemented MC, the removal percentage increased to 26% at 35 days. These results suggest that the microbial consortium with P-amendment is effective in CMZ degradation in MC, being a factor to consider for the herbicide removal in polluted and productive soils.
Fil: Cuadra, Pablo Nicolás. Universidad Tecnológica Nacional. Facultad Regional Resistencia. Grupo de Investigación Sobre Temas Ambientales y Químicos; Argentina.
Fil: Farías, Alejandro Rubén. Universidad Tecnológica Nacional. Facultad Regional Resistencia. Grupo de Investigación Sobre Temas Ambientales y Químicos; Argentina.
Fil: Echeverría, Macarena Celeste. Universidad Tecnológica Nacional. Facultad Regional Resistencia. Grupo de Investigación Sobre Temas Ambientales y Químicos; Argentina.
Fil: Jorge, Nelly Lidia. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura. Laboratorio de Investigaciones en Tecnología Ambiental; Argentina.
Fil: Vullo, Diana Lia. Universidad Nacional General Sarmiento. Instituto de Ciencias. Área de Química; Argentina.
description Clomazone (CMZ) is a selective pre and post emergence herbicide indicated for application in rice crops. Its incorrect use has been causing environmental problems, such as soil, groundwater and surface water persistent contamination. CMZ-resistant/tolerant native bacterial strains were previously isolated from rice crop soils in Las Palmas, Chaco, which were able to degrade this herbicide in batch cultures. The aim of this study was to evaluate CMZ biodegradation under controlled experiments in soil microcosms (MC) by bioaugmentation (the addition of specific microorganisms) and biostimulation (the addition of nutrients to increase microbial population activity) procedures. For this purpose, 30 MC were prepared by triplicates using 150 g of sterile and non-sterile rice crop soils under different treatments: control (non-treated MC), bioaugmentation, and a combination of bioaugmentation and biostimulation. The soil used exhibited a phosphorus deficit (8.4x10-4%), therefore biostimulated MC were designed by adding a stock solution of K2HPO4 1M to achieve the optimal C:N:P ratio of 100:10:1. For bioaugmented-MC, inocula consisted of strain G1 or a mixed culture of the isolates G1, H2, and H3, selected according to previous results. The MC were incubated at 30 °C and monitored for a period of 60 days. Bacterial growth was assessed by viable cell counts every 7 days, while CMZ concentration in soil was quantified by HPLC at regular intervals to determine CMZ removal. Results indicated that sterile soil control MC showed no detectable microbial growth until day 15, and CMZ concentration remained stable throughout the 60 days, confirming its abiotic stability under these conditions. In contrast, the other experimental conditions evidenced bacterial growth over time however no CMZ removal was registered in non sterile soil control MC. In G1 MC, G1 attained a maximum growth of 1×102 CFU/g soil at 14 days and of 1×103 CFU/g soil in P-supplemented MC, suggesting that P availability is a limiting factor. Furthermore, the G1-H3-H2-bioaugmented MC also achieved cell development of 1×103 CFU/g soil at 14 days, both with and without P-amendment indicating a greater intrinsic growth capacity of the consortium and a lesser dependence on external P supplementation. A clear predominance of isolate H2 was observed, followed by H3 and, to a lesser extent, G1, suggesting a dynamic of competition among the strains. Regarding the CMZ concentration analysis by HPLC, differentiated results were obtained: G1-MC did not show significant agrochemical degradation under any treatment condition. However, in the G1-H3-H2-MC a 14% CMZ removal was observed at 35 days without biostimulation, while in P-supplemented MC, the removal percentage increased to 26% at 35 days. These results suggest that the microbial consortium with P-amendment is effective in CMZ degradation in MC, being a factor to consider for the herbicide removal in polluted and productive soils.
publishDate 2025
dc.date.none.fl_str_mv 2025-12-19T18:55:29Z
2025-12-02
dc.type.none.fl_str_mv info:eu-repo/semantics/conferenceObject
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
format conferenceObject
status_str publishedVersion
dc.identifier.none.fl_str_mv XX Congreso Anual de la Sociedad Argentina de Microbiología General (SAMIGE 2025)
https://samige.org.ar/
https://hdl.handle.net/20.500.12272/14396
identifier_str_mv XX Congreso Anual de la Sociedad Argentina de Microbiología General (SAMIGE 2025)
url https://samige.org.ar/
https://hdl.handle.net/20.500.12272/14396
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv MSTCRE0008635TC
Biodegradación de herbicidas a nivel de microcosmos mediada por microorganismos nativos de suelos de cultivos de arroz
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
Acceso abierto
eu_rights_str_mv openAccess
rights_invalid_str_mv Acceso abierto
dc.format.none.fl_str_mv pdf
application/pdf
dc.publisher.none.fl_str_mv Sociedad Argentina de Microbiología General
publisher.none.fl_str_mv Sociedad Argentina de Microbiología General
dc.source.none.fl_str_mv reponame:Repositorio Institucional Abierto (UTN)
instname:Universidad Tecnológica Nacional
reponame_str Repositorio Institucional Abierto (UTN)
collection Repositorio Institucional Abierto (UTN)
instname_str Universidad Tecnológica Nacional
repository.name.fl_str_mv Repositorio Institucional Abierto (UTN) - Universidad Tecnológica Nacional
repository.mail.fl_str_mv gestionria@rec.utn.edu.ar; fsuarez@rec.utn.edu.ar
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