Selection of native bacteria from rice soils resistant to glyphosate, clomazone and Kifix, to be used as biodegrading agents

Autores
Cuadra, Pablo Nicolás; Fontana, Gimena Lilián; Farías, Alejandro Rubén; Jorge, Nelly Lidia; Vullo, Diana Lia
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Rice is the main crop worldwide, covering about 11% of the planet’s arable land and constitutes a strategic component of the diet adopted for more than half of the world population. In Argentina, 3% of its production is in the Province of Chaco. Glyphosate, clomazone and kifix, are herbicides commonly used in rice crops for weed control, generating alterations in the soil ecosystem, and affecting the trophic chain. In this province, several studies reported poisoning cases in humans by herbicide applications, particularly in La Leonesa. Consequently, local producers requested assistance to find a prompt solution for this environmental concern. As demonstrated, the ability of certain soil bacteria to degrade these types of pollutants ensures an onsite detoxification process. For this reason, the objective of this work was to isolate native bacteria from soils of rice crops, resistant to a mixture of agrochemicals composed by the commercial formulates of glyphosate, clomazone and kifix, to be applied in biodegradation tests. The soils samples were taken from 3 areas of a field intended for rice cultivation, located in La Leonesa: 1. Virgin area without registered pesticide applications (ZA), 2. Area without applications for a decade (ZB), and 3. Crop area with continuous application of agrochemicals (ZC). As a first approach, counts of cultivable microorganisms were carried out in a traditional nutrient medium (PCA) and an oligotrophic medium (AS= soil agar) with and without pesticides (CP and SP respectively). Differences were detected both in number and in the diversity according to the sampling area, the composition of the medium and the presence of pesticides. In parallel, mixed cultures were obtained from soil suspensions in a saline broth (M9) with 0.5% m/v glucose and 1% v/v of the same herbicides, obtaining new isolates in M9 agar (AM9). As a result, 24 strains were selected and purified from AS-CP, PCA-CP and AM9. Toxicity was evaluated by exposing these isolates to increasing concentrations of herbicides in agar cultures, registering a greater growth inhibition in AS-CP with respect to PCA and AM9, probably related to pesticide interactions with medium components lowering the bioavailability. From these tests, 16 strains were selected according to their resistance to the highest concentrations of the mixture (from 0.25% in AS-CP to 15% in PCA-CP). Finally, resistance assays were performed in M9 broth supplemented with increasing concentrations of herbicides (1-20% v/v), detecting 9 strains capable of developing up to 15% v/v. In further studies, pesticide biodegradation by-products will be screened in cultures supernatants. Regarding that the agrochemical applications in crops will eventually not be discontinued, this work represents the first step to develop an alternative based on native bacteria bioinoculants for the future quality recovery of Chaco rice production soils.
Fil: Cuadra, Pablo Nicolás. Universidad Tecnológica Nacional. Facultad Regional Resistencia. Grupo de Investigación Sobre Temas Ambientales y Químicos; Argentina.
Fil: Fontana, Gimena Lilián. Universidad Tecnológica Nacional. Facultad Regional Resistencia. Grupo de Investigación Sobre Temas Ambientales y Químicos; Argentina.
Fil: Farías, Alejandro Rubén. Universidad Tecnológica Nacional. Facultad Regional Resistencia. Grupo de Investigación Sobre Temas Ambientales y Químicos; Argentina.
Fil: Jorge, Nelly Lidia. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura. Laboratorio de Investigaciones en Tecnología Ambiental; Argentina.
Fil: Vullo, Diana Lia. Universidad Nacional General Sarmiento-CONICET. Instituto de Ciencias. Área de Química; Argentina
Peer Reviewed
Materia
native bacteria
herbicide toxicity
soil quality
biodegradation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
2024-03-21T00:15:53Z
Repositorio
Repositorio Institucional Abierto (UTN)
Institución
Universidad Tecnológica Nacional
OAI Identificador
oai:ria.utn.edu.ar:20.500.12272/9998
Acceder
id RIAUTN_de432893790b338daf5bdcd5386b8e33
oai_identifier_str oai:ria.utn.edu.ar:20.500.12272/9998
network_acronym_str RIAUTN
repository_id_str a
network_name_str Repositorio Institucional Abierto (UTN)
spelling Selection of native bacteria from rice soils resistant to glyphosate, clomazone and Kifix, to be used as biodegrading agentsCuadra, Pablo NicolásFontana, Gimena LiliánFarías, Alejandro RubénJorge, Nelly LidiaVullo, Diana Lianative bacteriaherbicide toxicitysoil qualitybiodegradationRice is the main crop worldwide, covering about 11% of the planet’s arable land and constitutes a strategic component of the diet adopted for more than half of the world population. In Argentina, 3% of its production is in the Province of Chaco. Glyphosate, clomazone and kifix, are herbicides commonly used in rice crops for weed control, generating alterations in the soil ecosystem, and affecting the trophic chain. In this province, several studies reported poisoning cases in humans by herbicide applications, particularly in La Leonesa. Consequently, local producers requested assistance to find a prompt solution for this environmental concern. As demonstrated, the ability of certain soil bacteria to degrade these types of pollutants ensures an onsite detoxification process. For this reason, the objective of this work was to isolate native bacteria from soils of rice crops, resistant to a mixture of agrochemicals composed by the commercial formulates of glyphosate, clomazone and kifix, to be applied in biodegradation tests. The soils samples were taken from 3 areas of a field intended for rice cultivation, located in La Leonesa: 1. Virgin area without registered pesticide applications (ZA), 2. Area without applications for a decade (ZB), and 3. Crop area with continuous application of agrochemicals (ZC). As a first approach, counts of cultivable microorganisms were carried out in a traditional nutrient medium (PCA) and an oligotrophic medium (AS= soil agar) with and without pesticides (CP and SP respectively). Differences were detected both in number and in the diversity according to the sampling area, the composition of the medium and the presence of pesticides. In parallel, mixed cultures were obtained from soil suspensions in a saline broth (M9) with 0.5% m/v glucose and 1% v/v of the same herbicides, obtaining new isolates in M9 agar (AM9). As a result, 24 strains were selected and purified from AS-CP, PCA-CP and AM9. Toxicity was evaluated by exposing these isolates to increasing concentrations of herbicides in agar cultures, registering a greater growth inhibition in AS-CP with respect to PCA and AM9, probably related to pesticide interactions with medium components lowering the bioavailability. From these tests, 16 strains were selected according to their resistance to the highest concentrations of the mixture (from 0.25% in AS-CP to 15% in PCA-CP). Finally, resistance assays were performed in M9 broth supplemented with increasing concentrations of herbicides (1-20% v/v), detecting 9 strains capable of developing up to 15% v/v. In further studies, pesticide biodegradation by-products will be screened in cultures supernatants. Regarding that the agrochemical applications in crops will eventually not be discontinued, this work represents the first step to develop an alternative based on native bacteria bioinoculants for the future quality recovery of Chaco rice production soils.Fil: Cuadra, Pablo Nicolás. Universidad Tecnológica Nacional. Facultad Regional Resistencia. Grupo de Investigación Sobre Temas Ambientales y Químicos; Argentina.Fil: Fontana, Gimena Lilián. Universidad Tecnológica Nacional. Facultad Regional Resistencia. Grupo de Investigación Sobre Temas Ambientales y Químicos; Argentina.Fil: Farías, Alejandro Rubén. Universidad Tecnológica Nacional. Facultad Regional Resistencia. Grupo de Investigación Sobre Temas Ambientales y Químicos; Argentina.Fil: Jorge, Nelly Lidia. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura. Laboratorio de Investigaciones en Tecnología Ambiental; Argentina.Fil: Vullo, Diana Lia. Universidad Nacional General Sarmiento-CONICET. Instituto de Ciencias. Área de Química; ArgentinaPeer Reviewed2024-03-21T00:15:53Z2024-03-21T00:15:53Z2022-10-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articulopdfapplication/pdfXVII Congreso Argentino de Microbiología General. Revista QuímicaViva1666-7948http://hdl.handle.net/20.500.12272/9998engengMSINIRE0006555TCRemoción biológica de residuos de agroquímicos ulizados en culvos regionalesinfo:eu-repo/semantics/openAccess2024-03-21T00:15:53Zhttp://creativecommons.org/licenses/by-nc-sa/4.0/Atribución-NoComercial-CompartirIgual 4.0 InternacionalAcceso abierto2022-12-01reponame:Repositorio Institucional Abierto (UTN)instname:Universidad Tecnológica Nacional2026-09-24T12:46:56Zoai:ria.utn.edu.ar:20.500.12272/9998instacron:UTNInstitucionalhttp://ria.utn.edu.ar/Universidad públicaNo correspondehttp://ria.utn.edu.ar/oaigestionria@rec.utn.edu.ar; fsuarez@rec.utn.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2026-09-24 12:46:57.318Repositorio Institucional Abierto (UTN) - Universidad Tecnológica Nacionalfalse
dc.title.none.fl_str_mv Selection of native bacteria from rice soils resistant to glyphosate, clomazone and Kifix, to be used as biodegrading agents
title Selection of native bacteria from rice soils resistant to glyphosate, clomazone and Kifix, to be used as biodegrading agents
spellingShingle Selection of native bacteria from rice soils resistant to glyphosate, clomazone and Kifix, to be used as biodegrading agents
Cuadra, Pablo Nicolás
native bacteria
herbicide toxicity
soil quality
biodegradation
title_short Selection of native bacteria from rice soils resistant to glyphosate, clomazone and Kifix, to be used as biodegrading agents
title_full Selection of native bacteria from rice soils resistant to glyphosate, clomazone and Kifix, to be used as biodegrading agents
title_fullStr Selection of native bacteria from rice soils resistant to glyphosate, clomazone and Kifix, to be used as biodegrading agents
title_full_unstemmed Selection of native bacteria from rice soils resistant to glyphosate, clomazone and Kifix, to be used as biodegrading agents
title_sort Selection of native bacteria from rice soils resistant to glyphosate, clomazone and Kifix, to be used as biodegrading agents
dc.creator.none.fl_str_mv Cuadra, Pablo Nicolás
Fontana, Gimena Lilián
Farías, Alejandro Rubén
Jorge, Nelly Lidia
Vullo, Diana Lia
author Cuadra, Pablo Nicolás
author_facet Cuadra, Pablo Nicolás
Fontana, Gimena Lilián
Farías, Alejandro Rubén
Jorge, Nelly Lidia
Vullo, Diana Lia
author_role author
author2 Fontana, Gimena Lilián
Farías, Alejandro Rubén
Jorge, Nelly Lidia
Vullo, Diana Lia
author2_role author
author
author
author
dc.subject.none.fl_str_mv native bacteria
herbicide toxicity
soil quality
biodegradation
topic native bacteria
herbicide toxicity
soil quality
biodegradation
dc.description.none.fl_txt_mv Rice is the main crop worldwide, covering about 11% of the planet’s arable land and constitutes a strategic component of the diet adopted for more than half of the world population. In Argentina, 3% of its production is in the Province of Chaco. Glyphosate, clomazone and kifix, are herbicides commonly used in rice crops for weed control, generating alterations in the soil ecosystem, and affecting the trophic chain. In this province, several studies reported poisoning cases in humans by herbicide applications, particularly in La Leonesa. Consequently, local producers requested assistance to find a prompt solution for this environmental concern. As demonstrated, the ability of certain soil bacteria to degrade these types of pollutants ensures an onsite detoxification process. For this reason, the objective of this work was to isolate native bacteria from soils of rice crops, resistant to a mixture of agrochemicals composed by the commercial formulates of glyphosate, clomazone and kifix, to be applied in biodegradation tests. The soils samples were taken from 3 areas of a field intended for rice cultivation, located in La Leonesa: 1. Virgin area without registered pesticide applications (ZA), 2. Area without applications for a decade (ZB), and 3. Crop area with continuous application of agrochemicals (ZC). As a first approach, counts of cultivable microorganisms were carried out in a traditional nutrient medium (PCA) and an oligotrophic medium (AS= soil agar) with and without pesticides (CP and SP respectively). Differences were detected both in number and in the diversity according to the sampling area, the composition of the medium and the presence of pesticides. In parallel, mixed cultures were obtained from soil suspensions in a saline broth (M9) with 0.5% m/v glucose and 1% v/v of the same herbicides, obtaining new isolates in M9 agar (AM9). As a result, 24 strains were selected and purified from AS-CP, PCA-CP and AM9. Toxicity was evaluated by exposing these isolates to increasing concentrations of herbicides in agar cultures, registering a greater growth inhibition in AS-CP with respect to PCA and AM9, probably related to pesticide interactions with medium components lowering the bioavailability. From these tests, 16 strains were selected according to their resistance to the highest concentrations of the mixture (from 0.25% in AS-CP to 15% in PCA-CP). Finally, resistance assays were performed in M9 broth supplemented with increasing concentrations of herbicides (1-20% v/v), detecting 9 strains capable of developing up to 15% v/v. In further studies, pesticide biodegradation by-products will be screened in cultures supernatants. Regarding that the agrochemical applications in crops will eventually not be discontinued, this work represents the first step to develop an alternative based on native bacteria bioinoculants for the future quality recovery of Chaco rice production soils.
Fil: Cuadra, Pablo Nicolás. Universidad Tecnológica Nacional. Facultad Regional Resistencia. Grupo de Investigación Sobre Temas Ambientales y Químicos; Argentina.
Fil: Fontana, Gimena Lilián. Universidad Tecnológica Nacional. Facultad Regional Resistencia. Grupo de Investigación Sobre Temas Ambientales y Químicos; Argentina.
Fil: Farías, Alejandro Rubén. Universidad Tecnológica Nacional. Facultad Regional Resistencia. Grupo de Investigación Sobre Temas Ambientales y Químicos; Argentina.
Fil: Jorge, Nelly Lidia. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura. Laboratorio de Investigaciones en Tecnología Ambiental; Argentina.
Fil: Vullo, Diana Lia. Universidad Nacional General Sarmiento-CONICET. Instituto de Ciencias. Área de Química; Argentina
Peer Reviewed
description Rice is the main crop worldwide, covering about 11% of the planet’s arable land and constitutes a strategic component of the diet adopted for more than half of the world population. In Argentina, 3% of its production is in the Province of Chaco. Glyphosate, clomazone and kifix, are herbicides commonly used in rice crops for weed control, generating alterations in the soil ecosystem, and affecting the trophic chain. In this province, several studies reported poisoning cases in humans by herbicide applications, particularly in La Leonesa. Consequently, local producers requested assistance to find a prompt solution for this environmental concern. As demonstrated, the ability of certain soil bacteria to degrade these types of pollutants ensures an onsite detoxification process. For this reason, the objective of this work was to isolate native bacteria from soils of rice crops, resistant to a mixture of agrochemicals composed by the commercial formulates of glyphosate, clomazone and kifix, to be applied in biodegradation tests. The soils samples were taken from 3 areas of a field intended for rice cultivation, located in La Leonesa: 1. Virgin area without registered pesticide applications (ZA), 2. Area without applications for a decade (ZB), and 3. Crop area with continuous application of agrochemicals (ZC). As a first approach, counts of cultivable microorganisms were carried out in a traditional nutrient medium (PCA) and an oligotrophic medium (AS= soil agar) with and without pesticides (CP and SP respectively). Differences were detected both in number and in the diversity according to the sampling area, the composition of the medium and the presence of pesticides. In parallel, mixed cultures were obtained from soil suspensions in a saline broth (M9) with 0.5% m/v glucose and 1% v/v of the same herbicides, obtaining new isolates in M9 agar (AM9). As a result, 24 strains were selected and purified from AS-CP, PCA-CP and AM9. Toxicity was evaluated by exposing these isolates to increasing concentrations of herbicides in agar cultures, registering a greater growth inhibition in AS-CP with respect to PCA and AM9, probably related to pesticide interactions with medium components lowering the bioavailability. From these tests, 16 strains were selected according to their resistance to the highest concentrations of the mixture (from 0.25% in AS-CP to 15% in PCA-CP). Finally, resistance assays were performed in M9 broth supplemented with increasing concentrations of herbicides (1-20% v/v), detecting 9 strains capable of developing up to 15% v/v. In further studies, pesticide biodegradation by-products will be screened in cultures supernatants. Regarding that the agrochemical applications in crops will eventually not be discontinued, this work represents the first step to develop an alternative based on native bacteria bioinoculants for the future quality recovery of Chaco rice production soils.
publishDate 2022
dc.date.none.fl_str_mv 2022-10-28
2024-03-21T00:15:53Z
2024-03-21T00:15:53Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv XVII Congreso Argentino de Microbiología General. Revista QuímicaViva
1666-7948
http://hdl.handle.net/20.500.12272/9998
identifier_str_mv XVII Congreso Argentino de Microbiología General. Revista QuímicaViva
1666-7948
url http://hdl.handle.net/20.500.12272/9998
dc.language.none.fl_str_mv eng
eng
language eng
dc.relation.none.fl_str_mv MSINIRE0006555TC
Remoción biológica de residuos de agroquímicos ulizados en culvos regionales
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
2024-03-21T00:15:53Z
http://creativecommons.org/licenses/by-nc-sa/4.0/
Atribución-NoComercial-CompartirIgual 4.0 Internacional
Acceso abierto
2022-12-01
eu_rights_str_mv openAccess
rights_invalid_str_mv 2024-03-21T00:15:53Z
http://creativecommons.org/licenses/by-nc-sa/4.0/
Atribución-NoComercial-CompartirIgual 4.0 Internacional
Acceso abierto
2022-12-01
dc.format.none.fl_str_mv pdf
application/pdf
dc.source.none.fl_str_mv reponame:Repositorio Institucional Abierto (UTN)
instname:Universidad Tecnológica Nacional
reponame_str Repositorio Institucional Abierto (UTN)
collection Repositorio Institucional Abierto (UTN)
instname_str Universidad Tecnológica Nacional
repository.name.fl_str_mv Repositorio Institucional Abierto (UTN) - Universidad Tecnológica Nacional
repository.mail.fl_str_mv gestionria@rec.utn.edu.ar; fsuarez@rec.utn.edu.ar
_version_ 1877230930736709632
score 13.265058

Publicaciones similares