Production of γ-aminobutyric acid (GABA) by lactic acid bacteria strains isolated from traditional, starter-free dairy products made of raw milk

Autores
Valenzuela, J. A.; Flórez García, Ana Belén; Vázquez, Laura; Vasek, Olga Myriam; Mayo, B.
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Valenzuela, J. A. Instituto de Productos Lácteos de Asturias. Departamento de Microbiología y Bioquímica; España.
Fil: Valenzuela, J. A. Universidad Nacional del Nordeste. Instituto de Modelado e Innovación Tecnológica; Argentina.
Fil: Valenzuela, J. A. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Modelado e Innovación Tecnológica; Argentina.
Fil: Flórez García, Ana Belén. Instituto de Productos Lácteos de Asturias. Departamento de Microbiología y Bioquímica; España.
Fil: Vázquez, Laura. Instituto de Productos Lácteos de Asturias. Departamento de Microbiología y Bioquímica; España.
Fil: Vasek, Olga Myriam. Universidad Nacional del Nordeste. Instituto de Modelado e Innovación Tecnológica; Argentina.
Fil: Vasek, Olga Myriam. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Modelado e Innovación Tecnológica; Argentina.
Fil: Mayo, B. Instituto de Productos Lácteos de Asturias. Departamento de Microbiología y Bioquímica; España.
γ-Aminobutyric acid (GABA), an amino acid not used in protein synthesis, intervenes in several physiological functions and has both diuretic and calming effects in humans. Lactic acid bacteria (LAB) strains that produce GABA could be exploited for the manufacture of health-promoting GABA-enriched dairy products. In this study, 262 LAB strains isolated from traditional dairy products made from raw milk without starter cultures were screened for GABA production in culture media supplemented with 1% monosodium glutamate (MSG) using an enzymatic (GABase) method. About half of the strains (123) were found to be GABA producers. Of these, 24, among which were 16 Lactococcus lactis subsp. lactis and three Streptococcus thermophilus strains, produced >1 mM of GABA (range 1.01-2.81 mM) and were selected for further characterisation. GABA production was confirmed in most strains by culturing in 5 mM MSG followed by HPLC quantification. A majority of the strains were confirmed to be GABA producers by this method, although lower production levels were recorded. Using species-specific primers, the gene encoding glutamate decarboxylase (GAD) was PCR-amplified in all but one of the GABA producers analysed. Amplicons sequences were compared to one another and to those held in databases. Except for one Lactobacillus brevis strain, none of the 24 GABA producers investigated produced toxic biogenic amines, such as tyramine, histamine or cadaverine. They were therefore considered safe. Either alone, in mixtures, or in combination with industrial starter or adjunct cultures, these strains might be useful in the development of health-oriented dairy products.
Fuente
Beneficial Microbes, 2019, vol. 10, no. 5, p. 579-587.
Materia
Gamma-aminobutyric acid
GABA
Lactic acid bacteria
Starters
Adjunct cultures
Dairy products
Functional foods
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
Institución
Universidad Nacional del Nordeste
OAI Identificador
oai:repositorio.unne.edu.ar:123456789/30852
Acceder
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oai_identifier_str oai:repositorio.unne.edu.ar:123456789/30852
network_acronym_str RIUNNE
repository_id_str 4871
network_name_str Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
spelling Production of γ-aminobutyric acid (GABA) by lactic acid bacteria strains isolated from traditional, starter-free dairy products made of raw milkValenzuela, J. A.Flórez García, Ana BelénVázquez, LauraVasek, Olga MyriamMayo, B.Gamma-aminobutyric acidGABALactic acid bacteriaStartersAdjunct culturesDairy productsFunctional foodsFil: Valenzuela, J. A. Instituto de Productos Lácteos de Asturias. Departamento de Microbiología y Bioquímica; España.Fil: Valenzuela, J. A. Universidad Nacional del Nordeste. Instituto de Modelado e Innovación Tecnológica; Argentina.Fil: Valenzuela, J. A. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Modelado e Innovación Tecnológica; Argentina.Fil: Flórez García, Ana Belén. Instituto de Productos Lácteos de Asturias. Departamento de Microbiología y Bioquímica; España.Fil: Vázquez, Laura. Instituto de Productos Lácteos de Asturias. Departamento de Microbiología y Bioquímica; España.Fil: Vasek, Olga Myriam. Universidad Nacional del Nordeste. Instituto de Modelado e Innovación Tecnológica; Argentina.Fil: Vasek, Olga Myriam. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Modelado e Innovación Tecnológica; Argentina.Fil: Mayo, B. Instituto de Productos Lácteos de Asturias. Departamento de Microbiología y Bioquímica; España.γ-Aminobutyric acid (GABA), an amino acid not used in protein synthesis, intervenes in several physiological functions and has both diuretic and calming effects in humans. Lactic acid bacteria (LAB) strains that produce GABA could be exploited for the manufacture of health-promoting GABA-enriched dairy products. In this study, 262 LAB strains isolated from traditional dairy products made from raw milk without starter cultures were screened for GABA production in culture media supplemented with 1% monosodium glutamate (MSG) using an enzymatic (GABase) method. About half of the strains (123) were found to be GABA producers. Of these, 24, among which were 16 Lactococcus lactis subsp. lactis and three Streptococcus thermophilus strains, produced >1 mM of GABA (range 1.01-2.81 mM) and were selected for further characterisation. GABA production was confirmed in most strains by culturing in 5 mM MSG followed by HPLC quantification. A majority of the strains were confirmed to be GABA producers by this method, although lower production levels were recorded. Using species-specific primers, the gene encoding glutamate decarboxylase (GAD) was PCR-amplified in all but one of the GABA producers analysed. Amplicons sequences were compared to one another and to those held in databases. Except for one Lactobacillus brevis strain, none of the 24 GABA producers investigated produced toxic biogenic amines, such as tyramine, histamine or cadaverine. They were therefore considered safe. Either alone, in mixtures, or in combination with industrial starter or adjunct cultures, these strains might be useful in the development of health-oriented dairy products.Wageningen Academic Publishers2019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfValenzuela, J. A., et al., 2019. Production of γ-aminobutyric acid (GABA) by lactic acid bacteria strains isolated from traditional, starter-free dairy products made of raw milk. Beneficial Microbes. Wageningen: Wageningen Academic Publishers, vol. 10, no. 5, p. 579-587. ISSN 1876-2891.1876-2883http://repositorio.unne.edu.ar/handle/123456789/30852Beneficial Microbes, 2019, vol. 10, no. 5, p. 579-587.reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)instname:Universidad Nacional del Nordesteenghttps://doi.org/10.3920/BM2018.0176info:eu-repo/semantics/restrictedAccess2026-09-24T12:48:29Zoai:repositorio.unne.edu.ar:123456789/30852instacron:UNNEInstitucionalhttp://repositorio.unne.edu.ar/Universidad públicaNo correspondehttp://repositorio.unne.edu.ar/oaiososa@bib.unne.edu.ar;sergio.alegria@unne.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:48712026-09-24 12:48:29.732Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordestefalse
dc.title.none.fl_str_mv Production of γ-aminobutyric acid (GABA) by lactic acid bacteria strains isolated from traditional, starter-free dairy products made of raw milk
title Production of γ-aminobutyric acid (GABA) by lactic acid bacteria strains isolated from traditional, starter-free dairy products made of raw milk
spellingShingle Production of γ-aminobutyric acid (GABA) by lactic acid bacteria strains isolated from traditional, starter-free dairy products made of raw milk
Valenzuela, J. A.
Gamma-aminobutyric acid
GABA
Lactic acid bacteria
Starters
Adjunct cultures
Dairy products
Functional foods
title_short Production of γ-aminobutyric acid (GABA) by lactic acid bacteria strains isolated from traditional, starter-free dairy products made of raw milk
title_full Production of γ-aminobutyric acid (GABA) by lactic acid bacteria strains isolated from traditional, starter-free dairy products made of raw milk
title_fullStr Production of γ-aminobutyric acid (GABA) by lactic acid bacteria strains isolated from traditional, starter-free dairy products made of raw milk
title_full_unstemmed Production of γ-aminobutyric acid (GABA) by lactic acid bacteria strains isolated from traditional, starter-free dairy products made of raw milk
title_sort Production of γ-aminobutyric acid (GABA) by lactic acid bacteria strains isolated from traditional, starter-free dairy products made of raw milk
dc.creator.none.fl_str_mv Valenzuela, J. A.
Flórez García, Ana Belén
Vázquez, Laura
Vasek, Olga Myriam
Mayo, B.
author Valenzuela, J. A.
author_facet Valenzuela, J. A.
Flórez García, Ana Belén
Vázquez, Laura
Vasek, Olga Myriam
Mayo, B.
author_role author
author2 Flórez García, Ana Belén
Vázquez, Laura
Vasek, Olga Myriam
Mayo, B.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Gamma-aminobutyric acid
GABA
Lactic acid bacteria
Starters
Adjunct cultures
Dairy products
Functional foods
topic Gamma-aminobutyric acid
GABA
Lactic acid bacteria
Starters
Adjunct cultures
Dairy products
Functional foods
dc.description.none.fl_txt_mv Fil: Valenzuela, J. A. Instituto de Productos Lácteos de Asturias. Departamento de Microbiología y Bioquímica; España.
Fil: Valenzuela, J. A. Universidad Nacional del Nordeste. Instituto de Modelado e Innovación Tecnológica; Argentina.
Fil: Valenzuela, J. A. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Modelado e Innovación Tecnológica; Argentina.
Fil: Flórez García, Ana Belén. Instituto de Productos Lácteos de Asturias. Departamento de Microbiología y Bioquímica; España.
Fil: Vázquez, Laura. Instituto de Productos Lácteos de Asturias. Departamento de Microbiología y Bioquímica; España.
Fil: Vasek, Olga Myriam. Universidad Nacional del Nordeste. Instituto de Modelado e Innovación Tecnológica; Argentina.
Fil: Vasek, Olga Myriam. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Modelado e Innovación Tecnológica; Argentina.
Fil: Mayo, B. Instituto de Productos Lácteos de Asturias. Departamento de Microbiología y Bioquímica; España.
γ-Aminobutyric acid (GABA), an amino acid not used in protein synthesis, intervenes in several physiological functions and has both diuretic and calming effects in humans. Lactic acid bacteria (LAB) strains that produce GABA could be exploited for the manufacture of health-promoting GABA-enriched dairy products. In this study, 262 LAB strains isolated from traditional dairy products made from raw milk without starter cultures were screened for GABA production in culture media supplemented with 1% monosodium glutamate (MSG) using an enzymatic (GABase) method. About half of the strains (123) were found to be GABA producers. Of these, 24, among which were 16 Lactococcus lactis subsp. lactis and three Streptococcus thermophilus strains, produced >1 mM of GABA (range 1.01-2.81 mM) and were selected for further characterisation. GABA production was confirmed in most strains by culturing in 5 mM MSG followed by HPLC quantification. A majority of the strains were confirmed to be GABA producers by this method, although lower production levels were recorded. Using species-specific primers, the gene encoding glutamate decarboxylase (GAD) was PCR-amplified in all but one of the GABA producers analysed. Amplicons sequences were compared to one another and to those held in databases. Except for one Lactobacillus brevis strain, none of the 24 GABA producers investigated produced toxic biogenic amines, such as tyramine, histamine or cadaverine. They were therefore considered safe. Either alone, in mixtures, or in combination with industrial starter or adjunct cultures, these strains might be useful in the development of health-oriented dairy products.
description Fil: Valenzuela, J. A. Instituto de Productos Lácteos de Asturias. Departamento de Microbiología y Bioquímica; España.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv Valenzuela, J. A., et al., 2019. Production of γ-aminobutyric acid (GABA) by lactic acid bacteria strains isolated from traditional, starter-free dairy products made of raw milk. Beneficial Microbes. Wageningen: Wageningen Academic Publishers, vol. 10, no. 5, p. 579-587. ISSN 1876-2891.
1876-2883
http://repositorio.unne.edu.ar/handle/123456789/30852
identifier_str_mv Valenzuela, J. A., et al., 2019. Production of γ-aminobutyric acid (GABA) by lactic acid bacteria strains isolated from traditional, starter-free dairy products made of raw milk. Beneficial Microbes. Wageningen: Wageningen Academic Publishers, vol. 10, no. 5, p. 579-587. ISSN 1876-2891.
1876-2883
url http://repositorio.unne.edu.ar/handle/123456789/30852
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://doi.org/10.3920/BM2018.0176
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wageningen Academic Publishers
publisher.none.fl_str_mv Wageningen Academic Publishers
dc.source.none.fl_str_mv Beneficial Microbes, 2019, vol. 10, no. 5, p. 579-587.
reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
instname:Universidad Nacional del Nordeste
reponame_str Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
collection Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
instname_str Universidad Nacional del Nordeste
repository.name.fl_str_mv Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordeste
repository.mail.fl_str_mv ososa@bib.unne.edu.ar;sergio.alegria@unne.edu.ar
_version_ 1877230555379007488
score 13.24418

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