Gene expression changes in steroid-induced IOP elevation in bovine trabecular meshwork
- Autores
- Danias, John; Gerometta, Rosana María del Rosario; Ge, Yongchao; Ren, Lizhen; Panagis, Lampros; Mittag, Thomas Waldemar; Candia, Oscar A.; Podos, Steven Mansfield
- Año de publicación
- 2011
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Fil: Danias, John. State University of New York. Downstate Medical Center; United States of America.
Fil: Gerometta, Rosana María del Rosario. Universidad Nacional del Nordeste. Facultad de Medicina; Argentina.
Fil: Ge, Yongchao. Mount Sinai School of Medicine; United States of America.
Fil: Ren, Lizhen. State University of New York. Downstate Medical Center; United States of America.
Fil: Panagis, Lampros. State University of New York. Downstate Medical Center; United States of America.
Fil: Mittag, Thomas Waldemar. Mount Sinai School of Medicine; United States of America.
Fil: Candia, Oscar A. Universidad Nacional del Nordeste. Facultad de Medicina; Argentina.
Fil: Podos, Steven Mansfield. Mount Sinai Hospital; United States of America.
PURPOSE. To determine whether gene expression changes oc-cur in the trabecular meshwork (TM) of cow eyes with steroid-induced intraocular pressure (IOP) elevation. METHODS. Adult female Braford cows (n 4) were subjected to uniocular prednisolone acetate treatment for 6 weeks. IOP was monitored with an applanation tonometer. At the conclusion of the experiment, animals were euthanized, eyes were enu-cleated, and the TM was dissected and stored in an aqueous nontoxic tissue storage reagent. RNA was extracted and sub- jected to microarray analysis using commercial oligonucleotide bovine arrays. Some of the genes differentially expressed be-tween control and experimental eyes were confirmed by quan-titative RT-PCR and some of the respective proteins were studied by immunoblotting. RESULTS. IOP began to increase after 3 weeks of treatment, reaching a peak 2 weeks later. IOP differences between corti-costeroid-treated and fellow control eyes were 6 1 mm Hg (mean SD) at the conclusion of the study. Microarray analysis revealed that expression of 258 genes was upregulated, whereas expression of 187 genes was downregulated in the of eyes with steroid-induced IOP elevation. Genes identi- fied to be differentially xpressed include genes coding for cytoskeletal proteins, enzymes, growth and transcription fac- tors, as well as extracellular matrix proteins and immune re- sponse proteins. A number of relevant gene networks were detected by bioinformatic analysis. CONCLUSIONS. Steroid-induced IOP elevation alters gene expres- sion in the bovine TM. Identification of genes with changing expression in this model of open-angle glaucoma may help elucidate the primary changes occurring at the molecular level in this condition. - Fuente
- Investigative Ophthalmology & Visual Science, 2011, vol. 52, no. 12, p. 8636-8645.
- Materia
-
Trabecular meshwork
Gene expression
Intraocular pressure - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
- Repositorio
.jpg)
- Institución
- Universidad Nacional del Nordeste
- OAI Identificador
- oai:repositorio.unne.edu.ar:123456789/60909
Ver los metadatos del registro completo
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Gene expression changes in steroid-induced IOP elevation in bovine trabecular meshworkDanias, JohnGerometta, Rosana María del RosarioGe, YongchaoRen, LizhenPanagis, LamprosMittag, Thomas WaldemarCandia, Oscar A.Podos, Steven MansfieldTrabecular meshworkGene expressionIntraocular pressureFil: Danias, John. State University of New York. Downstate Medical Center; United States of America.Fil: Gerometta, Rosana María del Rosario. Universidad Nacional del Nordeste. Facultad de Medicina; Argentina.Fil: Ge, Yongchao. Mount Sinai School of Medicine; United States of America.Fil: Ren, Lizhen. State University of New York. Downstate Medical Center; United States of America.Fil: Panagis, Lampros. State University of New York. Downstate Medical Center; United States of America.Fil: Mittag, Thomas Waldemar. Mount Sinai School of Medicine; United States of America.Fil: Candia, Oscar A. Universidad Nacional del Nordeste. Facultad de Medicina; Argentina.Fil: Podos, Steven Mansfield. Mount Sinai Hospital; United States of America.PURPOSE. To determine whether gene expression changes oc-cur in the trabecular meshwork (TM) of cow eyes with steroid-induced intraocular pressure (IOP) elevation. METHODS. Adult female Braford cows (n 4) were subjected to uniocular prednisolone acetate treatment for 6 weeks. IOP was monitored with an applanation tonometer. At the conclusion of the experiment, animals were euthanized, eyes were enu-cleated, and the TM was dissected and stored in an aqueous nontoxic tissue storage reagent. RNA was extracted and sub- jected to microarray analysis using commercial oligonucleotide bovine arrays. Some of the genes differentially expressed be-tween control and experimental eyes were confirmed by quan-titative RT-PCR and some of the respective proteins were studied by immunoblotting. RESULTS. IOP began to increase after 3 weeks of treatment, reaching a peak 2 weeks later. IOP differences between corti-costeroid-treated and fellow control eyes were 6 1 mm Hg (mean SD) at the conclusion of the study. Microarray analysis revealed that expression of 258 genes was upregulated, whereas expression of 187 genes was downregulated in the of eyes with steroid-induced IOP elevation. Genes identi- fied to be differentially xpressed include genes coding for cytoskeletal proteins, enzymes, growth and transcription fac- tors, as well as extracellular matrix proteins and immune re- sponse proteins. A number of relevant gene networks were detected by bioinformatic analysis. CONCLUSIONS. Steroid-induced IOP elevation alters gene expres- sion in the bovine TM. Identification of genes with changing expression in this model of open-angle glaucoma may help elucidate the primary changes occurring at the molecular level in this condition.Association for Research in Vision and Ophthalmology2011-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfp. 8636-8645application/pdfDanias, John, et al., 2011. Gene expression changes in steroid-induced iop elevation in bovine trabecular meshwork. Investigative Ophthalmology & Visual Science. Brooklyn: Association for Research in Vision and Ophthalmology, vol. 52, no. 12, p. 8636-8645. E-ISSN 1552-5783.https://repositorio.unne.edu.ar/handle/123456789/60909Investigative Ophthalmology & Visual Science, 2011, vol. 52, no. 12, p. 8636-8645.reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)instname:Universidad Nacional del Nordesteenghttps://iovs.arvojournals.org/article.aspx?articleid=2186796info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/2.5/ar/Atribución-NoComercial-SinDerivadas 2.5 Argentina2026-09-24T12:44:32Zoai:repositorio.unne.edu.ar:123456789/60909instacron:UNNEInstitucionalhttp://repositorio.unne.edu.ar/Universidad públicaNo correspondehttp://repositorio.unne.edu.ar/oaiososa@bib.unne.edu.ar;sergio.alegria@unne.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:48712026-09-24 12:44:33.611Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordestefalse |
| dc.title.none.fl_str_mv |
Gene expression changes in steroid-induced IOP elevation in bovine trabecular meshwork |
| title |
Gene expression changes in steroid-induced IOP elevation in bovine trabecular meshwork |
| spellingShingle |
Gene expression changes in steroid-induced IOP elevation in bovine trabecular meshwork Danias, John Trabecular meshwork Gene expression Intraocular pressure |
| title_short |
Gene expression changes in steroid-induced IOP elevation in bovine trabecular meshwork |
| title_full |
Gene expression changes in steroid-induced IOP elevation in bovine trabecular meshwork |
| title_fullStr |
Gene expression changes in steroid-induced IOP elevation in bovine trabecular meshwork |
| title_full_unstemmed |
Gene expression changes in steroid-induced IOP elevation in bovine trabecular meshwork |
| title_sort |
Gene expression changes in steroid-induced IOP elevation in bovine trabecular meshwork |
| dc.creator.none.fl_str_mv |
Danias, John Gerometta, Rosana María del Rosario Ge, Yongchao Ren, Lizhen Panagis, Lampros Mittag, Thomas Waldemar Candia, Oscar A. Podos, Steven Mansfield |
| author |
Danias, John |
| author_facet |
Danias, John Gerometta, Rosana María del Rosario Ge, Yongchao Ren, Lizhen Panagis, Lampros Mittag, Thomas Waldemar Candia, Oscar A. Podos, Steven Mansfield |
| author_role |
author |
| author2 |
Gerometta, Rosana María del Rosario Ge, Yongchao Ren, Lizhen Panagis, Lampros Mittag, Thomas Waldemar Candia, Oscar A. Podos, Steven Mansfield |
| author2_role |
author author author author author author author |
| dc.subject.none.fl_str_mv |
Trabecular meshwork Gene expression Intraocular pressure |
| topic |
Trabecular meshwork Gene expression Intraocular pressure |
| dc.description.none.fl_txt_mv |
Fil: Danias, John. State University of New York. Downstate Medical Center; United States of America. Fil: Gerometta, Rosana María del Rosario. Universidad Nacional del Nordeste. Facultad de Medicina; Argentina. Fil: Ge, Yongchao. Mount Sinai School of Medicine; United States of America. Fil: Ren, Lizhen. State University of New York. Downstate Medical Center; United States of America. Fil: Panagis, Lampros. State University of New York. Downstate Medical Center; United States of America. Fil: Mittag, Thomas Waldemar. Mount Sinai School of Medicine; United States of America. Fil: Candia, Oscar A. Universidad Nacional del Nordeste. Facultad de Medicina; Argentina. Fil: Podos, Steven Mansfield. Mount Sinai Hospital; United States of America. PURPOSE. To determine whether gene expression changes oc-cur in the trabecular meshwork (TM) of cow eyes with steroid-induced intraocular pressure (IOP) elevation. METHODS. Adult female Braford cows (n 4) were subjected to uniocular prednisolone acetate treatment for 6 weeks. IOP was monitored with an applanation tonometer. At the conclusion of the experiment, animals were euthanized, eyes were enu-cleated, and the TM was dissected and stored in an aqueous nontoxic tissue storage reagent. RNA was extracted and sub- jected to microarray analysis using commercial oligonucleotide bovine arrays. Some of the genes differentially expressed be-tween control and experimental eyes were confirmed by quan-titative RT-PCR and some of the respective proteins were studied by immunoblotting. RESULTS. IOP began to increase after 3 weeks of treatment, reaching a peak 2 weeks later. IOP differences between corti-costeroid-treated and fellow control eyes were 6 1 mm Hg (mean SD) at the conclusion of the study. Microarray analysis revealed that expression of 258 genes was upregulated, whereas expression of 187 genes was downregulated in the of eyes with steroid-induced IOP elevation. Genes identi- fied to be differentially xpressed include genes coding for cytoskeletal proteins, enzymes, growth and transcription fac- tors, as well as extracellular matrix proteins and immune re- sponse proteins. A number of relevant gene networks were detected by bioinformatic analysis. CONCLUSIONS. Steroid-induced IOP elevation alters gene expres- sion in the bovine TM. Identification of genes with changing expression in this model of open-angle glaucoma may help elucidate the primary changes occurring at the molecular level in this condition. |
| description |
Fil: Danias, John. State University of New York. Downstate Medical Center; United States of America. |
| publishDate |
2011 |
| dc.date.none.fl_str_mv |
2011-11 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
Danias, John, et al., 2011. Gene expression changes in steroid-induced iop elevation in bovine trabecular meshwork. Investigative Ophthalmology & Visual Science. Brooklyn: Association for Research in Vision and Ophthalmology, vol. 52, no. 12, p. 8636-8645. E-ISSN 1552-5783. https://repositorio.unne.edu.ar/handle/123456789/60909 |
| identifier_str_mv |
Danias, John, et al., 2011. Gene expression changes in steroid-induced iop elevation in bovine trabecular meshwork. Investigative Ophthalmology & Visual Science. Brooklyn: Association for Research in Vision and Ophthalmology, vol. 52, no. 12, p. 8636-8645. E-ISSN 1552-5783. |
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https://repositorio.unne.edu.ar/handle/123456789/60909 |
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eng |
| language |
eng |
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https://iovs.arvojournals.org/article.aspx?articleid=2186796 |
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info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ Atribución-NoComercial-SinDerivadas 2.5 Argentina |
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openAccess |
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http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ Atribución-NoComercial-SinDerivadas 2.5 Argentina |
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application/pdf p. 8636-8645 application/pdf |
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Association for Research in Vision and Ophthalmology |
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Association for Research in Vision and Ophthalmology |
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Investigative Ophthalmology & Visual Science, 2011, vol. 52, no. 12, p. 8636-8645. reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) instname:Universidad Nacional del Nordeste |
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Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordeste |
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