Reduction of steroid-induced intraocular pressure elevation in sheep by tissue plasminogen activator

Autores
Gerometta, Rosana María del Rosario; Kumar, Sandeep; Shah, Shaily; Alvarez, Larry; Candia, Oscar A.; Danias, John
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Gerometta, Rosana María del Rosario. Universidad Nacional del Nordeste. Facultad de Medicina; Argentina.
Fil: Gerometta, Rosana María del Rosario. Mount Sinai School of Medicine; United States of America.
Fil: Kumar, Sandeep. State University of New York. Downstate Medical Center; United States of America.
Fil: Shah, Shaily. State University of New York. Downstate Medical Center; United States of America.
Fil: Alvarez, Larry. Mount Sinai School of Medicine; United States of America.
Fil: Candia, Oscar A. Mount Sinai School of Medicine; United States of America.
Fil: Danias, John. State University of New York. Downstate Medical Center; United States of America.
PURPOSE. To investigate whether tissue plasminogen activator (tPA) can prevent and/or reverse steroid-induced IOP elevation in an ovine model. METHODS. Three animal groups were subjected to bilateral steroid-induced IOP elevation using thrice daily topical ocular prednisolone administration. In the first group (N 1⁄4 8), one eye each of two sheep was injected intravitreally with 100 lg, 200 lg, 500 lg, or 1 mg human recombinant tPA, while contralateral eyes received vehicle. In the second group (N 1⁄4 2), one eye was injected intravitreally with tPA (100 lg), while contralateral eyes received vehicle containing L-arginine. In the third group (N 1⁄4 4), each animal received intravitreal tPA in one eye concurrently with initiation of bilateral steroid administration. IOP was monitored for the duration of the experiment. Tissues from eyes of the third group were used to determine relative gene expression. RESULTS. In the first and second groups, IOP decreased by 9.7 (62.8) and 9.7 (61.6) mm Hg, respectively, 24 hours after tPA administration. In the third group, tPA-treated eyes did not develop IOP elevation with DIOP of 11.8 (61.3) mm Hg 8 days later. In all tPA-treated eyes, IOP remained low until the end of the study. mRNA levels in the trabecular meshwork were decreased for plasminogen activator tissue (PLAT), increased for matrix-metalloproteinase 1 (MMP-1), and stable for plasminogen activator inhibitor 1 (PAI-1), MMP-2, MMP-9, and MMP-13 in tPA-treated eyes compared with contralateral controls. PAI-1 mRNA levels in ciliary processes also remained similar. CONCLUSIONS. Recombinant human tPA is effective in both preventing and reversing steroid-induced IOP elevation in sheep. Tissue plasminogen activator may be useful as a therapeutic agent in steroid-induced glaucoma.
Fuente
Investigative Ophthalmology & Visual Science, 2013, vol. 53, no. 13, p. 7903-7909.
Materia
Intraocular pressure
Tissue plasminogen activator
Trabecular meshwork
Extracellular matrix
Matrix metalloproteinase
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
Institución
Universidad Nacional del Nordeste
OAI Identificador
oai:repositorio.unne.edu.ar:123456789/60962
Acceder
id RIUNNE_fb84d648313e7e8da556084fc745c8a9
oai_identifier_str oai:repositorio.unne.edu.ar:123456789/60962
network_acronym_str RIUNNE
repository_id_str 4871
network_name_str Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
spelling Reduction of steroid-induced intraocular pressure elevation in sheep by tissue plasminogen activatorGerometta, Rosana María del RosarioKumar, SandeepShah, ShailyAlvarez, LarryCandia, Oscar A.Danias, JohnIntraocular pressureTissue plasminogen activatorTrabecular meshworkExtracellular matrixMatrix metalloproteinaseFil: Gerometta, Rosana María del Rosario. Universidad Nacional del Nordeste. Facultad de Medicina; Argentina.Fil: Gerometta, Rosana María del Rosario. Mount Sinai School of Medicine; United States of America.Fil: Kumar, Sandeep. State University of New York. Downstate Medical Center; United States of America.Fil: Shah, Shaily. State University of New York. Downstate Medical Center; United States of America.Fil: Alvarez, Larry. Mount Sinai School of Medicine; United States of America.Fil: Candia, Oscar A. Mount Sinai School of Medicine; United States of America.Fil: Danias, John. State University of New York. Downstate Medical Center; United States of America.PURPOSE. To investigate whether tissue plasminogen activator (tPA) can prevent and/or reverse steroid-induced IOP elevation in an ovine model. METHODS. Three animal groups were subjected to bilateral steroid-induced IOP elevation using thrice daily topical ocular prednisolone administration. In the first group (N 1⁄4 8), one eye each of two sheep was injected intravitreally with 100 lg, 200 lg, 500 lg, or 1 mg human recombinant tPA, while contralateral eyes received vehicle. In the second group (N 1⁄4 2), one eye was injected intravitreally with tPA (100 lg), while contralateral eyes received vehicle containing L-arginine. In the third group (N 1⁄4 4), each animal received intravitreal tPA in one eye concurrently with initiation of bilateral steroid administration. IOP was monitored for the duration of the experiment. Tissues from eyes of the third group were used to determine relative gene expression. RESULTS. In the first and second groups, IOP decreased by 9.7 (62.8) and 9.7 (61.6) mm Hg, respectively, 24 hours after tPA administration. In the third group, tPA-treated eyes did not develop IOP elevation with DIOP of 11.8 (61.3) mm Hg 8 days later. In all tPA-treated eyes, IOP remained low until the end of the study. mRNA levels in the trabecular meshwork were decreased for plasminogen activator tissue (PLAT), increased for matrix-metalloproteinase 1 (MMP-1), and stable for plasminogen activator inhibitor 1 (PAI-1), MMP-2, MMP-9, and MMP-13 in tPA-treated eyes compared with contralateral controls. PAI-1 mRNA levels in ciliary processes also remained similar. CONCLUSIONS. Recombinant human tPA is effective in both preventing and reversing steroid-induced IOP elevation in sheep. Tissue plasminogen activator may be useful as a therapeutic agent in steroid-induced glaucoma.Association for Research in Vision and Ophthalmology2013-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfp. 7903-7909application/pdfGerometta, Rosana María del Rosario, et al., 2013. Reduction of steroid-induced intraocular pressure elevation in sheep by tissue plasminogen activator. Investigative Ophthalmology & Visual Science. Brooklyn: Association for Research in Vision and Ophthalmology, vol. 53, no. 13, p. 7903-7909. ISSN 1552-5783.1552-5783https://repositorio.unne.edu.ar/handle/123456789/60962Investigative Ophthalmology & Visual Science, 2013, vol. 53, no. 13, p. 7903-7909.reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)instname:Universidad Nacional del Nordesteenghttps://doi.org/10.1167/iovs.13-12801info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/2.5/ar/Atribución-NoComercial-SinDerivadas 2.5 Argentina2026-09-24T12:45:09Zoai:repositorio.unne.edu.ar:123456789/60962instacron:UNNEInstitucionalhttp://repositorio.unne.edu.ar/Universidad públicaNo correspondehttp://repositorio.unne.edu.ar/oaiososa@bib.unne.edu.ar;sergio.alegria@unne.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:48712026-09-24 12:45:10.771Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordestefalse
dc.title.none.fl_str_mv Reduction of steroid-induced intraocular pressure elevation in sheep by tissue plasminogen activator
title Reduction of steroid-induced intraocular pressure elevation in sheep by tissue plasminogen activator
spellingShingle Reduction of steroid-induced intraocular pressure elevation in sheep by tissue plasminogen activator
Gerometta, Rosana María del Rosario
Intraocular pressure
Tissue plasminogen activator
Trabecular meshwork
Extracellular matrix
Matrix metalloproteinase
title_short Reduction of steroid-induced intraocular pressure elevation in sheep by tissue plasminogen activator
title_full Reduction of steroid-induced intraocular pressure elevation in sheep by tissue plasminogen activator
title_fullStr Reduction of steroid-induced intraocular pressure elevation in sheep by tissue plasminogen activator
title_full_unstemmed Reduction of steroid-induced intraocular pressure elevation in sheep by tissue plasminogen activator
title_sort Reduction of steroid-induced intraocular pressure elevation in sheep by tissue plasminogen activator
dc.creator.none.fl_str_mv Gerometta, Rosana María del Rosario
Kumar, Sandeep
Shah, Shaily
Alvarez, Larry
Candia, Oscar A.
Danias, John
author Gerometta, Rosana María del Rosario
author_facet Gerometta, Rosana María del Rosario
Kumar, Sandeep
Shah, Shaily
Alvarez, Larry
Candia, Oscar A.
Danias, John
author_role author
author2 Kumar, Sandeep
Shah, Shaily
Alvarez, Larry
Candia, Oscar A.
Danias, John
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Intraocular pressure
Tissue plasminogen activator
Trabecular meshwork
Extracellular matrix
Matrix metalloproteinase
topic Intraocular pressure
Tissue plasminogen activator
Trabecular meshwork
Extracellular matrix
Matrix metalloproteinase
dc.description.none.fl_txt_mv Fil: Gerometta, Rosana María del Rosario. Universidad Nacional del Nordeste. Facultad de Medicina; Argentina.
Fil: Gerometta, Rosana María del Rosario. Mount Sinai School of Medicine; United States of America.
Fil: Kumar, Sandeep. State University of New York. Downstate Medical Center; United States of America.
Fil: Shah, Shaily. State University of New York. Downstate Medical Center; United States of America.
Fil: Alvarez, Larry. Mount Sinai School of Medicine; United States of America.
Fil: Candia, Oscar A. Mount Sinai School of Medicine; United States of America.
Fil: Danias, John. State University of New York. Downstate Medical Center; United States of America.
PURPOSE. To investigate whether tissue plasminogen activator (tPA) can prevent and/or reverse steroid-induced IOP elevation in an ovine model. METHODS. Three animal groups were subjected to bilateral steroid-induced IOP elevation using thrice daily topical ocular prednisolone administration. In the first group (N 1⁄4 8), one eye each of two sheep was injected intravitreally with 100 lg, 200 lg, 500 lg, or 1 mg human recombinant tPA, while contralateral eyes received vehicle. In the second group (N 1⁄4 2), one eye was injected intravitreally with tPA (100 lg), while contralateral eyes received vehicle containing L-arginine. In the third group (N 1⁄4 4), each animal received intravitreal tPA in one eye concurrently with initiation of bilateral steroid administration. IOP was monitored for the duration of the experiment. Tissues from eyes of the third group were used to determine relative gene expression. RESULTS. In the first and second groups, IOP decreased by 9.7 (62.8) and 9.7 (61.6) mm Hg, respectively, 24 hours after tPA administration. In the third group, tPA-treated eyes did not develop IOP elevation with DIOP of 11.8 (61.3) mm Hg 8 days later. In all tPA-treated eyes, IOP remained low until the end of the study. mRNA levels in the trabecular meshwork were decreased for plasminogen activator tissue (PLAT), increased for matrix-metalloproteinase 1 (MMP-1), and stable for plasminogen activator inhibitor 1 (PAI-1), MMP-2, MMP-9, and MMP-13 in tPA-treated eyes compared with contralateral controls. PAI-1 mRNA levels in ciliary processes also remained similar. CONCLUSIONS. Recombinant human tPA is effective in both preventing and reversing steroid-induced IOP elevation in sheep. Tissue plasminogen activator may be useful as a therapeutic agent in steroid-induced glaucoma.
description Fil: Gerometta, Rosana María del Rosario. Universidad Nacional del Nordeste. Facultad de Medicina; Argentina.
publishDate 2013
dc.date.none.fl_str_mv 2013-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv Gerometta, Rosana María del Rosario, et al., 2013. Reduction of steroid-induced intraocular pressure elevation in sheep by tissue plasminogen activator. Investigative Ophthalmology & Visual Science. Brooklyn: Association for Research in Vision and Ophthalmology, vol. 53, no. 13, p. 7903-7909. ISSN 1552-5783.
1552-5783
https://repositorio.unne.edu.ar/handle/123456789/60962
identifier_str_mv Gerometta, Rosana María del Rosario, et al., 2013. Reduction of steroid-induced intraocular pressure elevation in sheep by tissue plasminogen activator. Investigative Ophthalmology & Visual Science. Brooklyn: Association for Research in Vision and Ophthalmology, vol. 53, no. 13, p. 7903-7909. ISSN 1552-5783.
1552-5783
url https://repositorio.unne.edu.ar/handle/123456789/60962
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://doi.org/10.1167/iovs.13-12801
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Atribución-NoComercial-SinDerivadas 2.5 Argentina
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Atribución-NoComercial-SinDerivadas 2.5 Argentina
dc.format.none.fl_str_mv application/pdf
p. 7903-7909
application/pdf
dc.publisher.none.fl_str_mv Association for Research in Vision and Ophthalmology
publisher.none.fl_str_mv Association for Research in Vision and Ophthalmology
dc.source.none.fl_str_mv Investigative Ophthalmology & Visual Science, 2013, vol. 53, no. 13, p. 7903-7909.
reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
instname:Universidad Nacional del Nordeste
reponame_str Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
collection Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
instname_str Universidad Nacional del Nordeste
repository.name.fl_str_mv Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordeste
repository.mail.fl_str_mv ososa@bib.unne.edu.ar;sergio.alegria@unne.edu.ar
_version_ 1877230437890260992
score 13.24418

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