Evaluation of xylanse, alpha-amylase and cellulase production from Cellulosimicrobium sp. using a sweet potatoe root residues medium.

Autores
Conde Molina, Débora; Tubio, Gisela; Corbino, Graciela
Año de publicación
2021
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión aceptada
Descripción
Enzymes such as xylanase, alpha-amylase and cellulase are widely used in the food industry, mainly in baking. Most enzyme production processes have focused on fungi as producer of enzymes, however, bacterial producers (have) been less studied. On the other hand, search for low-cost and easily available raw materials that can be used as fermentable substrates is one of the most interesting challenges in biotechnology. In this work, we evaluated a biotechnological process for the valorization of sweet potato root residues as carbon source in order to obtain xylanase, alpha-amylase and cellulose from Cellulosimicrobium sp. The three enzymes evaluated were detected from Cellulosimicrobium sp. CO1A1 when bacteria were harvested at 5 days of incubation. Enzyme activities were not detected in the supernatant culture, however, they were registered in the cell pellet, being 2.1 ± 0.1 U/mL for xylanase, 1.6 ± 0.1 U/mL for alpha-amylase and 0.8 ± 0.1 U/mL for cellulose. We conclude that Cellulosimicrobium sp. CO1A1 is able to produce xylanase, alpha-amylase and cellulose using an alternative low-cost carbon source. Further testing will be needed to study xylanase production from Cellulosimicrobium sp. in order to generate a value-added product from the transformation of a residue product of agricultural activity.
Fil: Conde Molina, Débora. Universidad Tecnológica Nacional. Facultad Regional Delta. Laboratorio de Biotecnología; Argentina.
Fil: Tubio, Gisela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina.
Fil: Corbino, Graciela. Instituto Nacional de Tecnología Agropecuaria. Estación Experimental Agropecuaria San Pedro; Argentina.
Materia
UTN
Facultad Regional Delta
Sweet potato root
Cellulosimicrobium sp.
Alpha-amylase
Xylanase
Cellulase.
Nivel de accesibilidad
acceso abierto
Condiciones de uso
2022-02-22T19:55:50Z
Repositorio
Repositorio Institucional Abierto (UTN)
Institución
Universidad Tecnológica Nacional
OAI Identificador
oai:ria.utn.edu.ar:20.500.12272/6001
Acceder
id RIAUTN_359c8fbe94f7a1a536a80fc83751833f
oai_identifier_str oai:ria.utn.edu.ar:20.500.12272/6001
network_acronym_str RIAUTN
repository_id_str a
network_name_str Repositorio Institucional Abierto (UTN)
spelling Evaluation of xylanse, alpha-amylase and cellulase production from Cellulosimicrobium sp. using a sweet potatoe root residues medium.Conde Molina, DéboraTubio, GiselaCorbino, GracielaUTNFacultad Regional DeltaSweet potato rootCellulosimicrobium sp.Alpha-amylaseXylanaseCellulase.Enzymes such as xylanase, alpha-amylase and cellulase are widely used in the food industry, mainly in baking. Most enzyme production processes have focused on fungi as producer of enzymes, however, bacterial producers (have) been less studied. On the other hand, search for low-cost and easily available raw materials that can be used as fermentable substrates is one of the most interesting challenges in biotechnology. In this work, we evaluated a biotechnological process for the valorization of sweet potato root residues as carbon source in order to obtain xylanase, alpha-amylase and cellulose from Cellulosimicrobium sp. The three enzymes evaluated were detected from Cellulosimicrobium sp. CO1A1 when bacteria were harvested at 5 days of incubation. Enzyme activities were not detected in the supernatant culture, however, they were registered in the cell pellet, being 2.1 ± 0.1 U/mL for xylanase, 1.6 ± 0.1 U/mL for alpha-amylase and 0.8 ± 0.1 U/mL for cellulose. We conclude that Cellulosimicrobium sp. CO1A1 is able to produce xylanase, alpha-amylase and cellulose using an alternative low-cost carbon source. Further testing will be needed to study xylanase production from Cellulosimicrobium sp. in order to generate a value-added product from the transformation of a residue product of agricultural activity.Fil: Conde Molina, Débora. Universidad Tecnológica Nacional. Facultad Regional Delta. Laboratorio de Biotecnología; Argentina.Fil: Tubio, Gisela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina.Fil: Corbino, Graciela. Instituto Nacional de Tecnología Agropecuaria. Estación Experimental Agropecuaria San Pedro; Argentina.NUFT2022-02-22T19:55:50Z2022-02-22T19:55:50Z2021-09info:eu-repo/semantics/conferenceObjectinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciapdfapplication/pdfConde Molina D., Tubio G., Corbino G. (2021) Evaluation of xylanse, alpha-amylase and cellulase production from Cellulosimicrobium sp. using a sweet potatoe root residues medium. Proceedings of the Іst International Scientific and Practical Conference “Problems and practical approaches to the production and regulation of the use of food additives in the European Union countries and in Ukraine” in term of the EU Erasmus+ project Jean Monnet module. Kyiv, Ukraine.978-966-612-271-4http://hdl.handle.net/20.500.12272/6001engenginfo:eu-repo/semantics/openAccess2022-02-22T19:55:50Zhttp://creativecommons.org/licenses/by-nc-sa/4.0/Atribución-NoComercial-CompartirIgual 4.0 InternacionalLos autoresAtribución – No Comercial – Sin Obra Derivada (by-nc-nd)reponame:Repositorio Institucional Abierto (UTN)instname:Universidad Tecnológica Nacional2026-09-24T12:44:05Zoai:ria.utn.edu.ar:20.500.12272/6001instacron:UTNInstitucionalhttp://ria.utn.edu.ar/Universidad públicaNo correspondehttp://ria.utn.edu.ar/oaigestionria@rec.utn.edu.ar; fsuarez@rec.utn.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2026-09-24 12:44:07.119Repositorio Institucional Abierto (UTN) - Universidad Tecnológica Nacionalfalse
dc.title.none.fl_str_mv Evaluation of xylanse, alpha-amylase and cellulase production from Cellulosimicrobium sp. using a sweet potatoe root residues medium.
title Evaluation of xylanse, alpha-amylase and cellulase production from Cellulosimicrobium sp. using a sweet potatoe root residues medium.
spellingShingle Evaluation of xylanse, alpha-amylase and cellulase production from Cellulosimicrobium sp. using a sweet potatoe root residues medium.
Conde Molina, Débora
UTN
Facultad Regional Delta
Sweet potato root
Cellulosimicrobium sp.
Alpha-amylase
Xylanase
Cellulase.
title_short Evaluation of xylanse, alpha-amylase and cellulase production from Cellulosimicrobium sp. using a sweet potatoe root residues medium.
title_full Evaluation of xylanse, alpha-amylase and cellulase production from Cellulosimicrobium sp. using a sweet potatoe root residues medium.
title_fullStr Evaluation of xylanse, alpha-amylase and cellulase production from Cellulosimicrobium sp. using a sweet potatoe root residues medium.
title_full_unstemmed Evaluation of xylanse, alpha-amylase and cellulase production from Cellulosimicrobium sp. using a sweet potatoe root residues medium.
title_sort Evaluation of xylanse, alpha-amylase and cellulase production from Cellulosimicrobium sp. using a sweet potatoe root residues medium.
dc.creator.none.fl_str_mv Conde Molina, Débora
Tubio, Gisela
Corbino, Graciela
author Conde Molina, Débora
author_facet Conde Molina, Débora
Tubio, Gisela
Corbino, Graciela
author_role author
author2 Tubio, Gisela
Corbino, Graciela
author2_role author
author
dc.subject.none.fl_str_mv UTN
Facultad Regional Delta
Sweet potato root
Cellulosimicrobium sp.
Alpha-amylase
Xylanase
Cellulase.
topic UTN
Facultad Regional Delta
Sweet potato root
Cellulosimicrobium sp.
Alpha-amylase
Xylanase
Cellulase.
dc.description.none.fl_txt_mv Enzymes such as xylanase, alpha-amylase and cellulase are widely used in the food industry, mainly in baking. Most enzyme production processes have focused on fungi as producer of enzymes, however, bacterial producers (have) been less studied. On the other hand, search for low-cost and easily available raw materials that can be used as fermentable substrates is one of the most interesting challenges in biotechnology. In this work, we evaluated a biotechnological process for the valorization of sweet potato root residues as carbon source in order to obtain xylanase, alpha-amylase and cellulose from Cellulosimicrobium sp. The three enzymes evaluated were detected from Cellulosimicrobium sp. CO1A1 when bacteria were harvested at 5 days of incubation. Enzyme activities were not detected in the supernatant culture, however, they were registered in the cell pellet, being 2.1 ± 0.1 U/mL for xylanase, 1.6 ± 0.1 U/mL for alpha-amylase and 0.8 ± 0.1 U/mL for cellulose. We conclude that Cellulosimicrobium sp. CO1A1 is able to produce xylanase, alpha-amylase and cellulose using an alternative low-cost carbon source. Further testing will be needed to study xylanase production from Cellulosimicrobium sp. in order to generate a value-added product from the transformation of a residue product of agricultural activity.
Fil: Conde Molina, Débora. Universidad Tecnológica Nacional. Facultad Regional Delta. Laboratorio de Biotecnología; Argentina.
Fil: Tubio, Gisela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina.
Fil: Corbino, Graciela. Instituto Nacional de Tecnología Agropecuaria. Estación Experimental Agropecuaria San Pedro; Argentina.
description Enzymes such as xylanase, alpha-amylase and cellulase are widely used in the food industry, mainly in baking. Most enzyme production processes have focused on fungi as producer of enzymes, however, bacterial producers (have) been less studied. On the other hand, search for low-cost and easily available raw materials that can be used as fermentable substrates is one of the most interesting challenges in biotechnology. In this work, we evaluated a biotechnological process for the valorization of sweet potato root residues as carbon source in order to obtain xylanase, alpha-amylase and cellulose from Cellulosimicrobium sp. The three enzymes evaluated were detected from Cellulosimicrobium sp. CO1A1 when bacteria were harvested at 5 days of incubation. Enzyme activities were not detected in the supernatant culture, however, they were registered in the cell pellet, being 2.1 ± 0.1 U/mL for xylanase, 1.6 ± 0.1 U/mL for alpha-amylase and 0.8 ± 0.1 U/mL for cellulose. We conclude that Cellulosimicrobium sp. CO1A1 is able to produce xylanase, alpha-amylase and cellulose using an alternative low-cost carbon source. Further testing will be needed to study xylanase production from Cellulosimicrobium sp. in order to generate a value-added product from the transformation of a residue product of agricultural activity.
publishDate 2021
dc.date.none.fl_str_mv 2021-09
2022-02-22T19:55:50Z
2022-02-22T19:55:50Z
dc.type.none.fl_str_mv info:eu-repo/semantics/conferenceObject
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
format conferenceObject
status_str acceptedVersion
dc.identifier.none.fl_str_mv Conde Molina D., Tubio G., Corbino G. (2021) Evaluation of xylanse, alpha-amylase and cellulase production from Cellulosimicrobium sp. using a sweet potatoe root residues medium. Proceedings of the Іst International Scientific and Practical Conference “Problems and practical approaches to the production and regulation of the use of food additives in the European Union countries and in Ukraine” in term of the EU Erasmus+ project Jean Monnet module. Kyiv, Ukraine.
978-966-612-271-4
http://hdl.handle.net/20.500.12272/6001
identifier_str_mv Conde Molina D., Tubio G., Corbino G. (2021) Evaluation of xylanse, alpha-amylase and cellulase production from Cellulosimicrobium sp. using a sweet potatoe root residues medium. Proceedings of the Іst International Scientific and Practical Conference “Problems and practical approaches to the production and regulation of the use of food additives in the European Union countries and in Ukraine” in term of the EU Erasmus+ project Jean Monnet module. Kyiv, Ukraine.
978-966-612-271-4
url http://hdl.handle.net/20.500.12272/6001
dc.language.none.fl_str_mv eng
eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
2022-02-22T19:55:50Z
http://creativecommons.org/licenses/by-nc-sa/4.0/
Atribución-NoComercial-CompartirIgual 4.0 Internacional
Los autores
Atribución – No Comercial – Sin Obra Derivada (by-nc-nd)
eu_rights_str_mv openAccess
rights_invalid_str_mv 2022-02-22T19:55:50Z
http://creativecommons.org/licenses/by-nc-sa/4.0/
Atribución-NoComercial-CompartirIgual 4.0 Internacional
Los autores
Atribución – No Comercial – Sin Obra Derivada (by-nc-nd)
dc.format.none.fl_str_mv pdf
application/pdf
dc.publisher.none.fl_str_mv NUFT
publisher.none.fl_str_mv NUFT
dc.source.none.fl_str_mv reponame:Repositorio Institucional Abierto (UTN)
instname:Universidad Tecnológica Nacional
reponame_str Repositorio Institucional Abierto (UTN)
collection Repositorio Institucional Abierto (UTN)
instname_str Universidad Tecnológica Nacional
repository.name.fl_str_mv Repositorio Institucional Abierto (UTN) - Universidad Tecnológica Nacional
repository.mail.fl_str_mv gestionria@rec.utn.edu.ar; fsuarez@rec.utn.edu.ar
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