Sweet potato root waste: evaluation of a culture medium to produce xylanases from Cellulosimicrobium sp. CO1A1.

Autores
Conde Molina, Debora; Sanchez Hulmedilla, Betiana; Bogao, Guillermina; Tubio, Gisela; Corbino, Graciela
Año de publicación
2022
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión borrador
Descripción
The development of bioprocesses using agro-industrial waste is one of the most interesting challenges in biotechnology today, as it leads to the reuse of waste in an environmentally responsible way and the development of high value-added products. In the San Pedro region, sweet potato production generates considerable volumes of waste, as approximately 40% of the total production is discarded because it does not meet the requirements for commercialisation. In this study, the ability of the strain Cellulosimicrobium sp. CO1A1 to produce xylanases in a medium formulated with sweet potato root residues was evaluated; xylanase being an enzyme of great industrial application. Cellulosimicrobium sp. CO1A1 was grown in minimal saline medium containing 5 % (w/v) sweet potato root extract, at 135 rpm and 25 °C. Xylanase activity was determined using beech xylan (1%) as substrate at 50 ºC, reducing sugars released were determined by the Nelson-Somogyi method. Several assays were performed in order to evaluate xylanase activity, such as the analysis of cell fractions (supernatant -extracellular activity-, cell pellet -cell-associated activity- and cell homogenate -intracellular activity-); culture samples taken at 2, 5 and 7 days of incubation; and media formulated with different sweet potato varieties (Arapey, Beauregard, Covington, Selecta, Morada, Boni). In addition, the xylanase reaction was carried out in two buffer conditions, sodium citrate (50 mM, pH 5.2) and sodium phosphate (50 mM, pH 6.8). The results showed that although Cellulosimicrobium sp. CO1A1 showed growth in the medium formulated with sweet potato root, thus proving that it has the ability to use sweet potato root as a carbon source, it did not show the ability to produce xylanases under the conditions tested, unlike other strains of the genus Cellulosimicrobium reported.
Fil: Conde Molina, Debora. Universidad Tecnológica Nacional. Facultad Regional Delta. Grupo de Biotecnología y Nanotecnología Aplicada; Argentina.
Fil: Sanchez Hulmedilla, Betiana.Universidad Tecnológica Nacional. Facultad Regional Delta. Grupo de Biotecnología y Nanotecnología Aplicada; Argentina.
Fil: Bogao, Guillermina. Universidad Tecnológica Nacional. Facultad Regional Delta. Grupo de Biotecnología y Nanotecnología Aplicada; Argentina.
Fil: Tubio, Gisela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina.
Fil: Corbino, Graciela. Instituto Nacional de Tecnología Agropecuaria. Estación Experimental Agropecuaria San Pedro; Argentina.
Materia
Facultad Regional Delta
UTN
Sweet potato residues
Xylanases
Cellulosimicrobium sp.
Nivel de accesibilidad
acceso abierto
Condiciones de uso
2022-12-29T20:36:02Z
Repositorio
Repositorio Institucional Abierto (UTN)
Institución
Universidad Tecnológica Nacional
OAI Identificador
oai:ria.utn.edu.ar:20.500.12272/7470
Acceder
id RIAUTN_7cfb2a3eb9e468e8d5675ab38567388c
oai_identifier_str oai:ria.utn.edu.ar:20.500.12272/7470
network_acronym_str RIAUTN
repository_id_str a
network_name_str Repositorio Institucional Abierto (UTN)
spelling Sweet potato root waste: evaluation of a culture medium to produce xylanases from Cellulosimicrobium sp. CO1A1.Conde Molina, DeboraSanchez Hulmedilla, BetianaBogao, GuillerminaTubio, GiselaCorbino, GracielaFacultad Regional DeltaUTNSweet potato residuesXylanasesCellulosimicrobium sp.The development of bioprocesses using agro-industrial waste is one of the most interesting challenges in biotechnology today, as it leads to the reuse of waste in an environmentally responsible way and the development of high value-added products. In the San Pedro region, sweet potato production generates considerable volumes of waste, as approximately 40% of the total production is discarded because it does not meet the requirements for commercialisation. In this study, the ability of the strain Cellulosimicrobium sp. CO1A1 to produce xylanases in a medium formulated with sweet potato root residues was evaluated; xylanase being an enzyme of great industrial application. Cellulosimicrobium sp. CO1A1 was grown in minimal saline medium containing 5 % (w/v) sweet potato root extract, at 135 rpm and 25 °C. Xylanase activity was determined using beech xylan (1%) as substrate at 50 ºC, reducing sugars released were determined by the Nelson-Somogyi method. Several assays were performed in order to evaluate xylanase activity, such as the analysis of cell fractions (supernatant -extracellular activity-, cell pellet -cell-associated activity- and cell homogenate -intracellular activity-); culture samples taken at 2, 5 and 7 days of incubation; and media formulated with different sweet potato varieties (Arapey, Beauregard, Covington, Selecta, Morada, Boni). In addition, the xylanase reaction was carried out in two buffer conditions, sodium citrate (50 mM, pH 5.2) and sodium phosphate (50 mM, pH 6.8). The results showed that although Cellulosimicrobium sp. CO1A1 showed growth in the medium formulated with sweet potato root, thus proving that it has the ability to use sweet potato root as a carbon source, it did not show the ability to produce xylanases under the conditions tested, unlike other strains of the genus Cellulosimicrobium reported.Fil: Conde Molina, Debora. Universidad Tecnológica Nacional. Facultad Regional Delta. Grupo de Biotecnología y Nanotecnología Aplicada; Argentina.Fil: Sanchez Hulmedilla, Betiana.Universidad Tecnológica Nacional. Facultad Regional Delta. Grupo de Biotecnología y Nanotecnología Aplicada; Argentina.Fil: Bogao, Guillermina. Universidad Tecnológica Nacional. Facultad Regional Delta. Grupo de Biotecnología y Nanotecnología Aplicada; Argentina.Fil: Tubio, Gisela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina.Fil: Corbino, Graciela. Instituto Nacional de Tecnología Agropecuaria. Estación Experimental Agropecuaria San Pedro; Argentina.UTN Facultad Regional San Francisco2022-12-29T20:36:02Z2022-12-29T20:36:02Z2022-08info:eu-repo/semantics/conferenceObjectinfo:eu-repo/semantics/drafthttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciapdfapplication/pdfSánchez Holmedilla B., Bogao G., Tubio G., Corbino G., Conde Molina D. Sweet potato root waste: evaluation of a culture medium to produce xylanases from Cellulosimicrobium sp. CO1A1. Jornadas de Ciencia y Tecnología. Córdoba, Argentina.http://hdl.handle.net/20.500.12272/7470enginfo:eu-repo/semantics/openAccess2022-12-29T20:36:02Zhttp://creativecommons.org/licenses/by-nc-nd/4.0/Attribution-NonCommercial-NoDerivatives 4.0 InternacionalLos autoresAtribución – No Comercial – Sin Obra Derivada (by-nc-nd)reponame:Repositorio Institucional Abierto (UTN)instname:Universidad Tecnológica Nacional2026-09-24T12:46:43Zoai:ria.utn.edu.ar:20.500.12272/7470instacron:UTNInstitucionalhttp://ria.utn.edu.ar/Universidad públicaNo correspondehttp://ria.utn.edu.ar/oaigestionria@rec.utn.edu.ar; fsuarez@rec.utn.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2026-09-24 12:46:44.061Repositorio Institucional Abierto (UTN) - Universidad Tecnológica Nacionalfalse
dc.title.none.fl_str_mv Sweet potato root waste: evaluation of a culture medium to produce xylanases from Cellulosimicrobium sp. CO1A1.
title Sweet potato root waste: evaluation of a culture medium to produce xylanases from Cellulosimicrobium sp. CO1A1.
spellingShingle Sweet potato root waste: evaluation of a culture medium to produce xylanases from Cellulosimicrobium sp. CO1A1.
Conde Molina, Debora
Facultad Regional Delta
UTN
Sweet potato residues
Xylanases
Cellulosimicrobium sp.
title_short Sweet potato root waste: evaluation of a culture medium to produce xylanases from Cellulosimicrobium sp. CO1A1.
title_full Sweet potato root waste: evaluation of a culture medium to produce xylanases from Cellulosimicrobium sp. CO1A1.
title_fullStr Sweet potato root waste: evaluation of a culture medium to produce xylanases from Cellulosimicrobium sp. CO1A1.
title_full_unstemmed Sweet potato root waste: evaluation of a culture medium to produce xylanases from Cellulosimicrobium sp. CO1A1.
title_sort Sweet potato root waste: evaluation of a culture medium to produce xylanases from Cellulosimicrobium sp. CO1A1.
dc.creator.none.fl_str_mv Conde Molina, Debora
Sanchez Hulmedilla, Betiana
Bogao, Guillermina
Tubio, Gisela
Corbino, Graciela
author Conde Molina, Debora
author_facet Conde Molina, Debora
Sanchez Hulmedilla, Betiana
Bogao, Guillermina
Tubio, Gisela
Corbino, Graciela
author_role author
author2 Sanchez Hulmedilla, Betiana
Bogao, Guillermina
Tubio, Gisela
Corbino, Graciela
author2_role author
author
author
author
dc.subject.none.fl_str_mv Facultad Regional Delta
UTN
Sweet potato residues
Xylanases
Cellulosimicrobium sp.
topic Facultad Regional Delta
UTN
Sweet potato residues
Xylanases
Cellulosimicrobium sp.
dc.description.none.fl_txt_mv The development of bioprocesses using agro-industrial waste is one of the most interesting challenges in biotechnology today, as it leads to the reuse of waste in an environmentally responsible way and the development of high value-added products. In the San Pedro region, sweet potato production generates considerable volumes of waste, as approximately 40% of the total production is discarded because it does not meet the requirements for commercialisation. In this study, the ability of the strain Cellulosimicrobium sp. CO1A1 to produce xylanases in a medium formulated with sweet potato root residues was evaluated; xylanase being an enzyme of great industrial application. Cellulosimicrobium sp. CO1A1 was grown in minimal saline medium containing 5 % (w/v) sweet potato root extract, at 135 rpm and 25 °C. Xylanase activity was determined using beech xylan (1%) as substrate at 50 ºC, reducing sugars released were determined by the Nelson-Somogyi method. Several assays were performed in order to evaluate xylanase activity, such as the analysis of cell fractions (supernatant -extracellular activity-, cell pellet -cell-associated activity- and cell homogenate -intracellular activity-); culture samples taken at 2, 5 and 7 days of incubation; and media formulated with different sweet potato varieties (Arapey, Beauregard, Covington, Selecta, Morada, Boni). In addition, the xylanase reaction was carried out in two buffer conditions, sodium citrate (50 mM, pH 5.2) and sodium phosphate (50 mM, pH 6.8). The results showed that although Cellulosimicrobium sp. CO1A1 showed growth in the medium formulated with sweet potato root, thus proving that it has the ability to use sweet potato root as a carbon source, it did not show the ability to produce xylanases under the conditions tested, unlike other strains of the genus Cellulosimicrobium reported.
Fil: Conde Molina, Debora. Universidad Tecnológica Nacional. Facultad Regional Delta. Grupo de Biotecnología y Nanotecnología Aplicada; Argentina.
Fil: Sanchez Hulmedilla, Betiana.Universidad Tecnológica Nacional. Facultad Regional Delta. Grupo de Biotecnología y Nanotecnología Aplicada; Argentina.
Fil: Bogao, Guillermina. Universidad Tecnológica Nacional. Facultad Regional Delta. Grupo de Biotecnología y Nanotecnología Aplicada; Argentina.
Fil: Tubio, Gisela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina.
Fil: Corbino, Graciela. Instituto Nacional de Tecnología Agropecuaria. Estación Experimental Agropecuaria San Pedro; Argentina.
description The development of bioprocesses using agro-industrial waste is one of the most interesting challenges in biotechnology today, as it leads to the reuse of waste in an environmentally responsible way and the development of high value-added products. In the San Pedro region, sweet potato production generates considerable volumes of waste, as approximately 40% of the total production is discarded because it does not meet the requirements for commercialisation. In this study, the ability of the strain Cellulosimicrobium sp. CO1A1 to produce xylanases in a medium formulated with sweet potato root residues was evaluated; xylanase being an enzyme of great industrial application. Cellulosimicrobium sp. CO1A1 was grown in minimal saline medium containing 5 % (w/v) sweet potato root extract, at 135 rpm and 25 °C. Xylanase activity was determined using beech xylan (1%) as substrate at 50 ºC, reducing sugars released were determined by the Nelson-Somogyi method. Several assays were performed in order to evaluate xylanase activity, such as the analysis of cell fractions (supernatant -extracellular activity-, cell pellet -cell-associated activity- and cell homogenate -intracellular activity-); culture samples taken at 2, 5 and 7 days of incubation; and media formulated with different sweet potato varieties (Arapey, Beauregard, Covington, Selecta, Morada, Boni). In addition, the xylanase reaction was carried out in two buffer conditions, sodium citrate (50 mM, pH 5.2) and sodium phosphate (50 mM, pH 6.8). The results showed that although Cellulosimicrobium sp. CO1A1 showed growth in the medium formulated with sweet potato root, thus proving that it has the ability to use sweet potato root as a carbon source, it did not show the ability to produce xylanases under the conditions tested, unlike other strains of the genus Cellulosimicrobium reported.
publishDate 2022
dc.date.none.fl_str_mv 2022-12-29T20:36:02Z
2022-12-29T20:36:02Z
2022-08
dc.type.none.fl_str_mv info:eu-repo/semantics/conferenceObject
info:eu-repo/semantics/draft
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
format conferenceObject
status_str draft
dc.identifier.none.fl_str_mv Sánchez Holmedilla B., Bogao G., Tubio G., Corbino G., Conde Molina D. Sweet potato root waste: evaluation of a culture medium to produce xylanases from Cellulosimicrobium sp. CO1A1. Jornadas de Ciencia y Tecnología. Córdoba, Argentina.
http://hdl.handle.net/20.500.12272/7470
identifier_str_mv Sánchez Holmedilla B., Bogao G., Tubio G., Corbino G., Conde Molina D. Sweet potato root waste: evaluation of a culture medium to produce xylanases from Cellulosimicrobium sp. CO1A1. Jornadas de Ciencia y Tecnología. Córdoba, Argentina.
url http://hdl.handle.net/20.500.12272/7470
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
2022-12-29T20:36:02Z
http://creativecommons.org/licenses/by-nc-nd/4.0/
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Los autores
Atribución – No Comercial – Sin Obra Derivada (by-nc-nd)
eu_rights_str_mv openAccess
rights_invalid_str_mv 2022-12-29T20:36:02Z
http://creativecommons.org/licenses/by-nc-nd/4.0/
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Los autores
Atribución – No Comercial – Sin Obra Derivada (by-nc-nd)
dc.format.none.fl_str_mv pdf
application/pdf
dc.publisher.none.fl_str_mv UTN Facultad Regional San Francisco
publisher.none.fl_str_mv UTN Facultad Regional San Francisco
dc.source.none.fl_str_mv reponame:Repositorio Institucional Abierto (UTN)
instname:Universidad Tecnológica Nacional
reponame_str Repositorio Institucional Abierto (UTN)
collection Repositorio Institucional Abierto (UTN)
instname_str Universidad Tecnológica Nacional
repository.name.fl_str_mv Repositorio Institucional Abierto (UTN) - Universidad Tecnológica Nacional
repository.mail.fl_str_mv gestionria@rec.utn.edu.ar; fsuarez@rec.utn.edu.ar
_version_ 1877230928082763776
score 13.265058

Publicaciones similares