Evaluation of a native strain isolated from chronically hydrocarbon-contaminated sites.

Autores
Conde Molina, Débora; Giullieti, Ana María; Quevedo, Carla
Año de publicación
2016
Idioma
español castellano
Tipo de recurso
documento de conferencia
Estado
versión aceptada
Descripción
The industrial center Zárate – Campana represents one of the most important petrochemical areas in Argentina with several companies carrying out petrochemical activities. Due to spills produced in the area during last 50 years, it has generated hydrocarbon pollution on the site. In order to evaluate growth conditions of a native strain from chronically hydrocarbon-contaminated sites, in the present work we studied an environmental strain identified in the genus, which can degrade crude oil. Different cultures conditions were assayed in Erlenmeyer flasks, containing minimal salt medium and supplemented with a mixture of hydrocarbons (HC) of various molecular weights as only carbon source. Ochrobactrum sp strain (currently being a molecularly characterized) was cultivated at two temperatures: 20 and 25 ºC, and several substrate concentrations, 2, 4.5, 6, 7, 8 y 10 % V/V. Cultures were kept at 135 rpm and pH=7 during 14 days. Bacterial growth was estimated by cell dry weight method, for this a sample of 18 mL culture broth was collected at every 24 h, drying at 80ºC. Many bacteria in nature have been found to be capable of degrading crude oil, using it as their sole carbon source. Experiment showed that this strain can use HC as its carbon source. The maximum biomass concentration was obtained when the bacteria was cultured in media with 4.5 % of HC at 20ºC and 25ºC (1,33 g/L and 1,75 g/L) respectively at day 8. In addition, a significant biomass concentration was observed too when were used 6% and 7 % of HC in both temperatures between 7 and 13 days of cultured. According to these results, the environmental-derived strain Ochrobactrum sp isolated from hydrocarbon contaminated sites, has the ability to degrade crude oil at high level concentrations, and this could have potential use in bioremediation technologies.
Fil: Conde Molina, Débora. Universidad Tecnológica Nacional. Facultad Regional Delta. Laboratorio de Biotecnología; Argentina.
Fil: Giullieti, Ana María. NANOBIOTEC. Instituto de Nanobiotecnología; Argentina.
Fil: Quevedo, Carla. Universidad Tecnológica Nacional. Facultad Regional Delta. Grupo de Procesos Biotecnológicos; Argentina.
Materia
UTN
Facultad Regional Delta
Bioremediation
Hydrocarbons
Ochrobactrum sp
Nivel de accesibilidad
acceso abierto
Condiciones de uso
2022-04-22T19:21:11Z
Repositorio
Repositorio Institucional Abierto (UTN)
Institución
Universidad Tecnológica Nacional
OAI Identificador
oai:ria.utn.edu.ar:20.500.12272/6169
Acceder
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network_acronym_str RIAUTN
repository_id_str a
network_name_str Repositorio Institucional Abierto (UTN)
spelling Evaluation of a native strain isolated from chronically hydrocarbon-contaminated sites.Conde Molina, DéboraGiullieti, Ana MaríaQuevedo, CarlaUTNFacultad Regional DeltaBioremediationHydrocarbonsOchrobactrum spThe industrial center Zárate – Campana represents one of the most important petrochemical areas in Argentina with several companies carrying out petrochemical activities. Due to spills produced in the area during last 50 years, it has generated hydrocarbon pollution on the site. In order to evaluate growth conditions of a native strain from chronically hydrocarbon-contaminated sites, in the present work we studied an environmental strain identified in the genus, which can degrade crude oil. Different cultures conditions were assayed in Erlenmeyer flasks, containing minimal salt medium and supplemented with a mixture of hydrocarbons (HC) of various molecular weights as only carbon source. Ochrobactrum sp strain (currently being a molecularly characterized) was cultivated at two temperatures: 20 and 25 ºC, and several substrate concentrations, 2, 4.5, 6, 7, 8 y 10 % V/V. Cultures were kept at 135 rpm and pH=7 during 14 days. Bacterial growth was estimated by cell dry weight method, for this a sample of 18 mL culture broth was collected at every 24 h, drying at 80ºC. Many bacteria in nature have been found to be capable of degrading crude oil, using it as their sole carbon source. Experiment showed that this strain can use HC as its carbon source. The maximum biomass concentration was obtained when the bacteria was cultured in media with 4.5 % of HC at 20ºC and 25ºC (1,33 g/L and 1,75 g/L) respectively at day 8. In addition, a significant biomass concentration was observed too when were used 6% and 7 % of HC in both temperatures between 7 and 13 days of cultured. According to these results, the environmental-derived strain Ochrobactrum sp isolated from hydrocarbon contaminated sites, has the ability to degrade crude oil at high level concentrations, and this could have potential use in bioremediation technologies.Fil: Conde Molina, Débora. Universidad Tecnológica Nacional. Facultad Regional Delta. Laboratorio de Biotecnología; Argentina.Fil: Giullieti, Ana María. NANOBIOTEC. Instituto de Nanobiotecnología; Argentina.Fil: Quevedo, Carla. Universidad Tecnológica Nacional. Facultad Regional Delta. Grupo de Procesos Biotecnológicos; Argentina.Universidad Nacional de General San Martín2022-04-22T19:21:11Z2022-04-22T19:21:11Z2016-07info:eu-repo/semantics/conferenceObjectinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciapdfapplication/pdfConde Molina D., Liporace F., Giullieti AM, Quevedo C. Evaluation of a native strain isolated from chronically hydrocarbon-contaminated sites. 5th International Symposium on Environmental Biotechnology and Engineering. 2016. Universidad Nacional de General San Martín, Buenos Aires, Argentina.http://hdl.handle.net/20.500.12272/6169spainfo:eu-repo/semantics/openAccess2022-04-22T19:21:11Zhttp://creativecommons.org/licenses/by-nc-sa/4.0/Atribución-NoComercial-CompartirIgual 4.0 InternacionalLos autoresAtribución – No Comercial – Sin Obra Derivada (by-nc-nd)reponame:Repositorio Institucional Abierto (UTN)instname:Universidad Tecnológica Nacional2026-09-24T12:45:37Zoai:ria.utn.edu.ar:20.500.12272/6169instacron:UTNInstitucionalhttp://ria.utn.edu.ar/Universidad públicaNo correspondehttp://ria.utn.edu.ar/oaigestionria@rec.utn.edu.ar; fsuarez@rec.utn.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2026-09-24 12:45:38.936Repositorio Institucional Abierto (UTN) - Universidad Tecnológica Nacionalfalse
dc.title.none.fl_str_mv Evaluation of a native strain isolated from chronically hydrocarbon-contaminated sites.
title Evaluation of a native strain isolated from chronically hydrocarbon-contaminated sites.
spellingShingle Evaluation of a native strain isolated from chronically hydrocarbon-contaminated sites.
Conde Molina, Débora
UTN
Facultad Regional Delta
Bioremediation
Hydrocarbons
Ochrobactrum sp
title_short Evaluation of a native strain isolated from chronically hydrocarbon-contaminated sites.
title_full Evaluation of a native strain isolated from chronically hydrocarbon-contaminated sites.
title_fullStr Evaluation of a native strain isolated from chronically hydrocarbon-contaminated sites.
title_full_unstemmed Evaluation of a native strain isolated from chronically hydrocarbon-contaminated sites.
title_sort Evaluation of a native strain isolated from chronically hydrocarbon-contaminated sites.
dc.creator.none.fl_str_mv Conde Molina, Débora
Giullieti, Ana María
Quevedo, Carla
author Conde Molina, Débora
author_facet Conde Molina, Débora
Giullieti, Ana María
Quevedo, Carla
author_role author
author2 Giullieti, Ana María
Quevedo, Carla
author2_role author
author
dc.subject.none.fl_str_mv UTN
Facultad Regional Delta
Bioremediation
Hydrocarbons
Ochrobactrum sp
topic UTN
Facultad Regional Delta
Bioremediation
Hydrocarbons
Ochrobactrum sp
dc.description.none.fl_txt_mv The industrial center Zárate – Campana represents one of the most important petrochemical areas in Argentina with several companies carrying out petrochemical activities. Due to spills produced in the area during last 50 years, it has generated hydrocarbon pollution on the site. In order to evaluate growth conditions of a native strain from chronically hydrocarbon-contaminated sites, in the present work we studied an environmental strain identified in the genus, which can degrade crude oil. Different cultures conditions were assayed in Erlenmeyer flasks, containing minimal salt medium and supplemented with a mixture of hydrocarbons (HC) of various molecular weights as only carbon source. Ochrobactrum sp strain (currently being a molecularly characterized) was cultivated at two temperatures: 20 and 25 ºC, and several substrate concentrations, 2, 4.5, 6, 7, 8 y 10 % V/V. Cultures were kept at 135 rpm and pH=7 during 14 days. Bacterial growth was estimated by cell dry weight method, for this a sample of 18 mL culture broth was collected at every 24 h, drying at 80ºC. Many bacteria in nature have been found to be capable of degrading crude oil, using it as their sole carbon source. Experiment showed that this strain can use HC as its carbon source. The maximum biomass concentration was obtained when the bacteria was cultured in media with 4.5 % of HC at 20ºC and 25ºC (1,33 g/L and 1,75 g/L) respectively at day 8. In addition, a significant biomass concentration was observed too when were used 6% and 7 % of HC in both temperatures between 7 and 13 days of cultured. According to these results, the environmental-derived strain Ochrobactrum sp isolated from hydrocarbon contaminated sites, has the ability to degrade crude oil at high level concentrations, and this could have potential use in bioremediation technologies.
Fil: Conde Molina, Débora. Universidad Tecnológica Nacional. Facultad Regional Delta. Laboratorio de Biotecnología; Argentina.
Fil: Giullieti, Ana María. NANOBIOTEC. Instituto de Nanobiotecnología; Argentina.
Fil: Quevedo, Carla. Universidad Tecnológica Nacional. Facultad Regional Delta. Grupo de Procesos Biotecnológicos; Argentina.
description The industrial center Zárate – Campana represents one of the most important petrochemical areas in Argentina with several companies carrying out petrochemical activities. Due to spills produced in the area during last 50 years, it has generated hydrocarbon pollution on the site. In order to evaluate growth conditions of a native strain from chronically hydrocarbon-contaminated sites, in the present work we studied an environmental strain identified in the genus, which can degrade crude oil. Different cultures conditions were assayed in Erlenmeyer flasks, containing minimal salt medium and supplemented with a mixture of hydrocarbons (HC) of various molecular weights as only carbon source. Ochrobactrum sp strain (currently being a molecularly characterized) was cultivated at two temperatures: 20 and 25 ºC, and several substrate concentrations, 2, 4.5, 6, 7, 8 y 10 % V/V. Cultures were kept at 135 rpm and pH=7 during 14 days. Bacterial growth was estimated by cell dry weight method, for this a sample of 18 mL culture broth was collected at every 24 h, drying at 80ºC. Many bacteria in nature have been found to be capable of degrading crude oil, using it as their sole carbon source. Experiment showed that this strain can use HC as its carbon source. The maximum biomass concentration was obtained when the bacteria was cultured in media with 4.5 % of HC at 20ºC and 25ºC (1,33 g/L and 1,75 g/L) respectively at day 8. In addition, a significant biomass concentration was observed too when were used 6% and 7 % of HC in both temperatures between 7 and 13 days of cultured. According to these results, the environmental-derived strain Ochrobactrum sp isolated from hydrocarbon contaminated sites, has the ability to degrade crude oil at high level concentrations, and this could have potential use in bioremediation technologies.
publishDate 2016
dc.date.none.fl_str_mv 2016-07
2022-04-22T19:21:11Z
2022-04-22T19:21:11Z
dc.type.none.fl_str_mv info:eu-repo/semantics/conferenceObject
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
format conferenceObject
status_str acceptedVersion
dc.identifier.none.fl_str_mv Conde Molina D., Liporace F., Giullieti AM, Quevedo C. Evaluation of a native strain isolated from chronically hydrocarbon-contaminated sites. 5th International Symposium on Environmental Biotechnology and Engineering. 2016. Universidad Nacional de General San Martín, Buenos Aires, Argentina.
http://hdl.handle.net/20.500.12272/6169
identifier_str_mv Conde Molina D., Liporace F., Giullieti AM, Quevedo C. Evaluation of a native strain isolated from chronically hydrocarbon-contaminated sites. 5th International Symposium on Environmental Biotechnology and Engineering. 2016. Universidad Nacional de General San Martín, Buenos Aires, Argentina.
url http://hdl.handle.net/20.500.12272/6169
dc.language.none.fl_str_mv spa
language spa
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
2022-04-22T19:21:11Z
http://creativecommons.org/licenses/by-nc-sa/4.0/
Atribución-NoComercial-CompartirIgual 4.0 Internacional
Los autores
Atribución – No Comercial – Sin Obra Derivada (by-nc-nd)
eu_rights_str_mv openAccess
rights_invalid_str_mv 2022-04-22T19:21:11Z
http://creativecommons.org/licenses/by-nc-sa/4.0/
Atribución-NoComercial-CompartirIgual 4.0 Internacional
Los autores
Atribución – No Comercial – Sin Obra Derivada (by-nc-nd)
dc.format.none.fl_str_mv pdf
application/pdf
dc.publisher.none.fl_str_mv Universidad Nacional de General San Martín
publisher.none.fl_str_mv Universidad Nacional de General San Martín
dc.source.none.fl_str_mv reponame:Repositorio Institucional Abierto (UTN)
instname:Universidad Tecnológica Nacional
reponame_str Repositorio Institucional Abierto (UTN)
collection Repositorio Institucional Abierto (UTN)
instname_str Universidad Tecnológica Nacional
repository.name.fl_str_mv Repositorio Institucional Abierto (UTN) - Universidad Tecnológica Nacional
repository.mail.fl_str_mv gestionria@rec.utn.edu.ar; fsuarez@rec.utn.edu.ar
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