Bioremediation strategies based on a native strain isolated from sites contaminated with hydrocarbons.

Autores
Conde Molina, Débora; Liporace, Franco; Vázquez, Susana; Merini, Luciano; Quevedo, Carla
Año de publicación
2016
Idioma
español castellano
Tipo de recurso
documento de conferencia
Estado
versión aceptada
Descripción
The bacterial strain studied in this work is a member of a bacterial consortium isolated from chronically hydrocarbon-contaminated site in Campana (Bs.As.). This native strain was identified as Pseudomonas sp. according to its 16S rRNA gene partial sequence. The ability of this strain to produce biosurfactants was evaluated in Erlenmeyer flasks containing a minimal saline medium (MSM) supplemented with different carbon sources: a mixture of hydrocarbons (HC; 4.5%v/v), glycerol (Gly; 2%v/v), sunflower oil (SO; 2%v/v) and peanut oil (PO; 2%v/v). Cultures were performed at 135 rpm and 25 ºC for 4 days. Bacterial growth was measured by cell dry-weight method, and biosurfactant production was estimated by direct measurement of the surface tension (ST). Results showed that bacteria was able to grow on all the carbon sources tested, reaching concentrations of 1.24g/l in HC, 7.69g/l in Gly, 3.98g/l in SO and 9.29g/l in PO. Culture supernatants showed a decrease in ST values when the strain grew on SO and PO (22.5% and 25.5%). No decrease in ST values was observed when HC and Gly were used as carbon source. As results shown, this bacterial strain can produce biosurfactants under certain culture conditions. This potential advantage could be applied in bioremediation strategy of hydrocarbon contaminated sites.
Fil: Conde Molina, Débora. Universidad Tecnológica Nacional. Facultad Regional Delta. Grupo de Procesos Biotecnológicos; Argentina.
Fil: Liporace, Franco. Universidad Tecnológica Nacional. Facultad Regional Delta. Grupo de Procesos Biotecnológicos; Argentina.
Fil: Vázquez, Susana. NANOBIOTEC. Instituto de Nanobiotecnología; Argentina.
Fil: Merini, Luciano. EEA - INTA; Argentina.
Fil: Quevedo, Carla. Universidad Tecnológica Nacional. Facultad Regional Delta. Grupo de Procesos Biotecnológicos; Argentina.
Materia
UTN
Facultad Regional Delta
Bioremediation
Native strain
Carbon sources
Nivel de accesibilidad
acceso abierto
Condiciones de uso
2022-04-22T18:48:42Z
Repositorio
Repositorio Institucional Abierto (UTN)
Institución
Universidad Tecnológica Nacional
OAI Identificador
oai:ria.utn.edu.ar:20.500.12272/6168
Acceder
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oai_identifier_str oai:ria.utn.edu.ar:20.500.12272/6168
network_acronym_str RIAUTN
repository_id_str a
network_name_str Repositorio Institucional Abierto (UTN)
spelling Bioremediation strategies based on a native strain isolated from sites contaminated with hydrocarbons.Conde Molina, DéboraLiporace, FrancoVázquez, SusanaMerini, LucianoQuevedo, CarlaUTNFacultad Regional DeltaBioremediationNative strainCarbon sourcesThe bacterial strain studied in this work is a member of a bacterial consortium isolated from chronically hydrocarbon-contaminated site in Campana (Bs.As.). This native strain was identified as Pseudomonas sp. according to its 16S rRNA gene partial sequence. The ability of this strain to produce biosurfactants was evaluated in Erlenmeyer flasks containing a minimal saline medium (MSM) supplemented with different carbon sources: a mixture of hydrocarbons (HC; 4.5%v/v), glycerol (Gly; 2%v/v), sunflower oil (SO; 2%v/v) and peanut oil (PO; 2%v/v). Cultures were performed at 135 rpm and 25 ºC for 4 days. Bacterial growth was measured by cell dry-weight method, and biosurfactant production was estimated by direct measurement of the surface tension (ST). Results showed that bacteria was able to grow on all the carbon sources tested, reaching concentrations of 1.24g/l in HC, 7.69g/l in Gly, 3.98g/l in SO and 9.29g/l in PO. Culture supernatants showed a decrease in ST values when the strain grew on SO and PO (22.5% and 25.5%). No decrease in ST values was observed when HC and Gly were used as carbon source. As results shown, this bacterial strain can produce biosurfactants under certain culture conditions. This potential advantage could be applied in bioremediation strategy of hydrocarbon contaminated sites.Fil: Conde Molina, Débora. Universidad Tecnológica Nacional. Facultad Regional Delta. Grupo de Procesos Biotecnológicos; Argentina.Fil: Liporace, Franco. Universidad Tecnológica Nacional. Facultad Regional Delta. Grupo de Procesos Biotecnológicos; Argentina.Fil: Vázquez, Susana. NANOBIOTEC. Instituto de Nanobiotecnología; Argentina.Fil: Merini, Luciano. EEA - INTA; Argentina.Fil: Quevedo, Carla. Universidad Tecnológica Nacional. Facultad Regional Delta. Grupo de Procesos Biotecnológicos; Argentina.Argentine Society for Biochemistry and Molecular Biology2022-04-22T18:48:42Z2022-04-22T18:48:42Z2016-11info:eu-repo/semantics/conferenceObjectinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciapdfapplication/pdfConde Molina D., Liporace F., Vázquez S., Merini L., Giuletti A., Quevedo C. Bioremediation strategies based on a native strain isolated from sites contaminated with hydrocarbons. 52th Annual Meeting Argentine Society for Biochemistry and Molecular Biology. Córdoba, Argentina. Biocell 40 (Suppl. 1), 78.http://hdl.handle.net/20.500.12272/6168spainfo:eu-repo/semantics/openAccess2022-04-22T18:48:42Zhttp://creativecommons.org/licenses/by-nc-sa/4.0/Atribución-NoComercial-CompartirIgual 4.0 InternacionalLos autoresAtribución – No Comercial – Sin Obra Derivada (by-nc-nd)reponame:Repositorio Institucional Abierto (UTN)instname:Universidad Tecnológica Nacional2026-09-24T12:47:49Zoai:ria.utn.edu.ar:20.500.12272/6168instacron:UTNInstitucionalhttp://ria.utn.edu.ar/Universidad públicaNo correspondehttp://ria.utn.edu.ar/oaigestionria@rec.utn.edu.ar; fsuarez@rec.utn.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2026-09-24 12:47:51.315Repositorio Institucional Abierto (UTN) - Universidad Tecnológica Nacionalfalse
dc.title.none.fl_str_mv Bioremediation strategies based on a native strain isolated from sites contaminated with hydrocarbons.
title Bioremediation strategies based on a native strain isolated from sites contaminated with hydrocarbons.
spellingShingle Bioremediation strategies based on a native strain isolated from sites contaminated with hydrocarbons.
Conde Molina, Débora
UTN
Facultad Regional Delta
Bioremediation
Native strain
Carbon sources
title_short Bioremediation strategies based on a native strain isolated from sites contaminated with hydrocarbons.
title_full Bioremediation strategies based on a native strain isolated from sites contaminated with hydrocarbons.
title_fullStr Bioremediation strategies based on a native strain isolated from sites contaminated with hydrocarbons.
title_full_unstemmed Bioremediation strategies based on a native strain isolated from sites contaminated with hydrocarbons.
title_sort Bioremediation strategies based on a native strain isolated from sites contaminated with hydrocarbons.
dc.creator.none.fl_str_mv Conde Molina, Débora
Liporace, Franco
Vázquez, Susana
Merini, Luciano
Quevedo, Carla
author Conde Molina, Débora
author_facet Conde Molina, Débora
Liporace, Franco
Vázquez, Susana
Merini, Luciano
Quevedo, Carla
author_role author
author2 Liporace, Franco
Vázquez, Susana
Merini, Luciano
Quevedo, Carla
author2_role author
author
author
author
dc.subject.none.fl_str_mv UTN
Facultad Regional Delta
Bioremediation
Native strain
Carbon sources
topic UTN
Facultad Regional Delta
Bioremediation
Native strain
Carbon sources
dc.description.none.fl_txt_mv The bacterial strain studied in this work is a member of a bacterial consortium isolated from chronically hydrocarbon-contaminated site in Campana (Bs.As.). This native strain was identified as Pseudomonas sp. according to its 16S rRNA gene partial sequence. The ability of this strain to produce biosurfactants was evaluated in Erlenmeyer flasks containing a minimal saline medium (MSM) supplemented with different carbon sources: a mixture of hydrocarbons (HC; 4.5%v/v), glycerol (Gly; 2%v/v), sunflower oil (SO; 2%v/v) and peanut oil (PO; 2%v/v). Cultures were performed at 135 rpm and 25 ºC for 4 days. Bacterial growth was measured by cell dry-weight method, and biosurfactant production was estimated by direct measurement of the surface tension (ST). Results showed that bacteria was able to grow on all the carbon sources tested, reaching concentrations of 1.24g/l in HC, 7.69g/l in Gly, 3.98g/l in SO and 9.29g/l in PO. Culture supernatants showed a decrease in ST values when the strain grew on SO and PO (22.5% and 25.5%). No decrease in ST values was observed when HC and Gly were used as carbon source. As results shown, this bacterial strain can produce biosurfactants under certain culture conditions. This potential advantage could be applied in bioremediation strategy of hydrocarbon contaminated sites.
Fil: Conde Molina, Débora. Universidad Tecnológica Nacional. Facultad Regional Delta. Grupo de Procesos Biotecnológicos; Argentina.
Fil: Liporace, Franco. Universidad Tecnológica Nacional. Facultad Regional Delta. Grupo de Procesos Biotecnológicos; Argentina.
Fil: Vázquez, Susana. NANOBIOTEC. Instituto de Nanobiotecnología; Argentina.
Fil: Merini, Luciano. EEA - INTA; Argentina.
Fil: Quevedo, Carla. Universidad Tecnológica Nacional. Facultad Regional Delta. Grupo de Procesos Biotecnológicos; Argentina.
description The bacterial strain studied in this work is a member of a bacterial consortium isolated from chronically hydrocarbon-contaminated site in Campana (Bs.As.). This native strain was identified as Pseudomonas sp. according to its 16S rRNA gene partial sequence. The ability of this strain to produce biosurfactants was evaluated in Erlenmeyer flasks containing a minimal saline medium (MSM) supplemented with different carbon sources: a mixture of hydrocarbons (HC; 4.5%v/v), glycerol (Gly; 2%v/v), sunflower oil (SO; 2%v/v) and peanut oil (PO; 2%v/v). Cultures were performed at 135 rpm and 25 ºC for 4 days. Bacterial growth was measured by cell dry-weight method, and biosurfactant production was estimated by direct measurement of the surface tension (ST). Results showed that bacteria was able to grow on all the carbon sources tested, reaching concentrations of 1.24g/l in HC, 7.69g/l in Gly, 3.98g/l in SO and 9.29g/l in PO. Culture supernatants showed a decrease in ST values when the strain grew on SO and PO (22.5% and 25.5%). No decrease in ST values was observed when HC and Gly were used as carbon source. As results shown, this bacterial strain can produce biosurfactants under certain culture conditions. This potential advantage could be applied in bioremediation strategy of hydrocarbon contaminated sites.
publishDate 2016
dc.date.none.fl_str_mv 2016-11
2022-04-22T18:48:42Z
2022-04-22T18:48:42Z
dc.type.none.fl_str_mv info:eu-repo/semantics/conferenceObject
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
format conferenceObject
status_str acceptedVersion
dc.identifier.none.fl_str_mv Conde Molina D., Liporace F., Vázquez S., Merini L., Giuletti A., Quevedo C. Bioremediation strategies based on a native strain isolated from sites contaminated with hydrocarbons. 52th Annual Meeting Argentine Society for Biochemistry and Molecular Biology. Córdoba, Argentina. Biocell 40 (Suppl. 1), 78.
http://hdl.handle.net/20.500.12272/6168
identifier_str_mv Conde Molina D., Liporace F., Vázquez S., Merini L., Giuletti A., Quevedo C. Bioremediation strategies based on a native strain isolated from sites contaminated with hydrocarbons. 52th Annual Meeting Argentine Society for Biochemistry and Molecular Biology. Córdoba, Argentina. Biocell 40 (Suppl. 1), 78.
url http://hdl.handle.net/20.500.12272/6168
dc.language.none.fl_str_mv spa
language spa
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
2022-04-22T18:48:42Z
http://creativecommons.org/licenses/by-nc-sa/4.0/
Atribución-NoComercial-CompartirIgual 4.0 Internacional
Los autores
Atribución – No Comercial – Sin Obra Derivada (by-nc-nd)
eu_rights_str_mv openAccess
rights_invalid_str_mv 2022-04-22T18:48:42Z
http://creativecommons.org/licenses/by-nc-sa/4.0/
Atribución-NoComercial-CompartirIgual 4.0 Internacional
Los autores
Atribución – No Comercial – Sin Obra Derivada (by-nc-nd)
dc.format.none.fl_str_mv pdf
application/pdf
dc.publisher.none.fl_str_mv Argentine Society for Biochemistry and Molecular Biology
publisher.none.fl_str_mv Argentine Society for Biochemistry and Molecular Biology
dc.source.none.fl_str_mv reponame:Repositorio Institucional Abierto (UTN)
instname:Universidad Tecnológica Nacional
reponame_str Repositorio Institucional Abierto (UTN)
collection Repositorio Institucional Abierto (UTN)
instname_str Universidad Tecnológica Nacional
repository.name.fl_str_mv Repositorio Institucional Abierto (UTN) - Universidad Tecnológica Nacional
repository.mail.fl_str_mv gestionria@rec.utn.edu.ar; fsuarez@rec.utn.edu.ar
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score 13.265058

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